Analysis of aspartate aminotransferase in gingival crevicular fluid: A study with initial therapy

Sheth, Tejal; Verma, Sharmila J

doi:10.4103/0972-124x.85666

Cited by 11 publications

(13 citation statements)

References 15 publications

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“…Our results also coincide with Mehta et al (23) who found similar results as Shimada et al concerning a positive relation between AST and periodontal diseases. We also have shown that there is a statistically significant correlation between mean periodontal probing depth (PPD) and the AST levels in Saliva at baseline (0-weeks) suggesting that during the diseased state, as the mean PPD increases, the mean AST levels in saliva also increase.…”

Section: Discussionsupporting

confidence: 93%

Evaluation of some salivary enzymes as a diagnostic biomarker for progression of periodontal disease.

Kader

Elhabbak

Assadawy

2021

Al-Azhar Journal of Dental Science

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Objectives: Saliva has been discussed lately as an important biological material to the purpose of diagnosis. The objective of our present study, was to study candidate biochemical markers, enzymes, in salivary samples for the screening of periodontal disease. These enzymes were ALT, AST, GGT, LDH and CK. Subjects and Methods: A total of sixty participants males and females and ranging in age from 30-45 years old were selected; 40 patients with periodontal disease and 20 healthy individuals were included in this study. Group I was comprised of 20 patients having periodontitis; forming the test group. Group II was comprised of 20 patients with gingivitis, forming the positive control group. Group III was comprised of 20 healthy adult volunteers who represented the negative control group. All individuals were assessed by: Gingival index (Loe and Sillness), probing depth and clinical attachment loss. Patients within periodontitis group underwent conventional periodontal treatment consisting of oral hygiene instructions, full mouth thorough scaling and root planning (SRP). The salivary samples were collected at baseline from all groups (before treatment) and three weeks post treatment of periodontitis group. Results: The activities of ALT, AST, GGT, LDH and CK enzymes were significantly increased in the saliva of patients with periodontal disease in relation to those of healthy condition, thus they can be used as a biological biomarker for progression of periodontal disease. This is probably a consequence of pathological processes in periodontal tissues where from these intracellular enzymes are increasingly released into the secretion which surrounds them -saliva. There was also an established correlation between AST and the value of the gingival index and probing depth. After periodontal treatment the activity of examined salivary enzymes was decreased, which is probably a result of periodontal tissues repair. Conclusion: Screening of periodontal disease by measuring salivary levels of ALT, AST, GGT, LDH and CK may be a feasible, simple and convenient approach that does not require expert examiners especially in mass screening events.

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Section: Discussionsupporting

confidence: 93%

Evaluation of some salivary enzymes as a diagnostic biomarker for progression of periodontal disease.

Kader

Elhabbak

Assadawy

2021

Al-Azhar Journal of Dental Science

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“…In patients with chronic periodontitis, three months [11] or one month [12], after the basic therapy, there was a decrease in the AST levels in the saliva. Similar results were obtained in the gingival fluid [14]. Results of this study indicate that the mean value of the AST activity in the saliva of patients with AP was reduced two months after the basic and surgical therapy.…”

Section: Discussionsupporting

confidence: 85%

“…et al [9] published the first study that indicated an increase in the AST levels in gingival fluid in dogs during the experimental periodontitis.Since that time, many researchers have found that the AST activity in the saliva is proportionate to the degree of tissue damage during the chronic periodontitis [10,11] and gingivitis [12]. Due to the pathological process in periodontal tissues, the integrity of the cells is impaired, the permeability of their membranes is disturbed, and thus the AST is increasingly released from the cytoplasm into the saliva.Also, the AST values in the saliva [13] and gingival fluid [14] were in correlation with the values of the CPITN index (Community Periodontal Index of Treatment Needs). Our study has not proved that there is a correlation between the values of clinical parameters and the AST activity in the saliva.…”

Section: Discussionmentioning

confidence: 99%

Analysis of biochemical markers in the saliva and correlation with clinical parameters in patients with aggressive periodontitis, before and after the therapy

et al. 2021

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Introduction/Objective. Aggressive periodontitis (AP) is a progressive disease that leads damage to periodontal tissues. The aim of the study was the analysis of intracellular enzymes: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), acid phosphatase (ACP) and electrolytes in the saliva of patients with AP and their correlation with clinical parameters before and after the therapy. Methods. The study included 30 patients with AP (experimental group) and 35 patients with healthy parodontium (control group). Intracellular enzymes and electrolytes were analyzed in an unstimulated saliva of subjects with AP, before and after the therapy and in saliva of the control group. The analysis of biochemical markers was carried out using kinetic methods with commercial reagents. Results. Concentrations of the biochemical markers AST (28.18 ? 25.16), ALT (5.48 ? 5.14), ALP (31.13 ? 37.79), ACP (17.53 ? 14.77), calcium (2.80 ? 1.97), phosphate (4.43 ? 1.92) in the saliva of subjects of the experimental group were statistically significantly higher in relation to the control group (p = 0.000; p = 0.001). Significant correlation was found between AST values, debris index (? = -0.444; p = 0.026) and calculus index (? = -0.513; p = 0.009), and between the plaque index and ALP level in the saliva after therapy (p = 0.020). Conclusion. The investigation will contribute to a better understanding and standardization of biomarkers in the saliva that mayhelp in diagnosing the AP and evaluation of the applied therapy.

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“…However, certain limitations could not be overcome, such as the choice of collection device, length of collection time, number of sample repetitions. [26][27][28] It is a known fact that the amount of GCF collected varies from site to site, and hence the volume collected differs. In this study, we addressed this issue by standardizing the time of collection to 30 seconds, yet the volume of fluid collected differed.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Systemic Doxycycline and Mechanical Therapy on GCF β-Glucuronidase Leves in Chronic Periodontitis Patients

et al. 2017

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The goal of periodontal therapy includes management of bacterial pathogens to control the destruction of the periodontal tissue. A number of different nonsurgical and surgical therapies have been used in achieving this goal. Objective: To evaluate the effectiveness of doxycycline compared with nonsurgical therapy alone or in combination with nonsurgical therapy on gingival crevicular fluid β-Glucuronidase levels (ßG) and clinical parameters over a 20-week period in patients with chronic periodontitis. Methods: An interventional study comprising 60 patients with chronic periodontitis. They were divided into three groups of different treatment approach. The plaque index, gingival index, probing depth, and clinical attachment levels were recorded, and gingival crevicular fluid (GCF) samples were collected at 2, 6, 10, and 20 weeks, after treatment. Results: The levels of GCF βG and clinical parameters were higher when the treatment was initiated, and the levels decreased after treatment. No significant difference was found in the βG level when nonsurgical therapy and doxycycline were used alone. In comparison, when doxycyline and nonsurgical therapy were combined, the level of βG decreased significantly. Conclusion: The present study indicated the usefulness of doxycycline as an adjunct to nonsurgical periodontal therapy in lowering GFC βG levels and clinical parameters.

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Analysis of aspartate aminotransferase in gingival crevicular fluid: A study with initial therapy

Cited by 11 publications

References 15 publications

Evaluation of some salivary enzymes as a diagnostic biomarker for progression of periodontal disease.

Evaluation of some salivary enzymes as a diagnostic biomarker for progression of periodontal disease.

Analysis of biochemical markers in the saliva and correlation with clinical parameters in patients with aggressive periodontitis, before and after the therapy

The Effect of Systemic Doxycycline and Mechanical Therapy on GCF β-Glucuronidase Leves in Chronic Periodontitis Patients

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