Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns

Rim, Yeonggil; Huang, Lijun; Chu, Hyosub; Han, Xiao; Cho, Won Kyong; Jeon, Che Ok; Kim, Hye Jin; Hong, Jong-Chan; Lucas, William J.; Kim, Jae‐Yean

doi:10.1007/s10059-011-0135-2

Cited by 51 publications

(34 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This extends findings described in previous reports that CPC-Dendra2 (a photoconvertible fluorescent protein) was able to move isodiametrically within the root epidermis (Wu et al, 2011) and that fluorescence signal was detected in all epidermal cells in EGL3p:CPC-GFP plants (Kurata et al, 2005a). Furthermore, we showed that CPC/CPC-GFP expressed in the root ground tissue or in the stele led to CPC-GFP accumulation in the root epidermis and alterations in epidermal cell fate specification, consistent with a report that fluorescence signal was detected in the epidermis and the stele when CPC-mCherry was expressed in root ground tissue (Rim et al, 2011). From this, we conclude that the CPC protein is able to move out from inner tissue layers of the root to the epidermis to affect cell fate specification.…”

Section: Movement Of Cpc Protein Within and Between Tissues In The Desupporting

confidence: 74%

Nuclear Trapping Controls the Position-Dependent Localization of CAPRICE in the Root Epidermis of Arabidopsis

et al. 2013

View full text Add to dashboard Cite

Cell fate determination and differentiation are central processes in the development of multicellular organisms, and the Arabidopsis (Arabidopsis thaliana) root epidermis provides a model system to study the molecular basis of these processes. A lateral inhibition mechanism mediated by an R3 single-repeat MYB protein, CAPRICE (CPC), has been proposed to explain the specification of the two types of root epidermal cells (hair cells and nonhair cells). However, it is not clear how CPC acts preferentially in the H-position cells, rather than the N-position cells, where its gene is expressed. To explore this issue, we examined the effect of misexpressed CPC on cell fate specification and CPC localization in the root epidermis. We show that CPC is able to move readily within the root epidermis when its expression level is high and that CPC can induce the hair cell fate in a cell-autonomous manner. We provide evidence that CPC is capable of moving from the stele tissue in the center of the root to the outermost epidermal layer, where it can induce the hair cell fate. In addition, we show that CPC protein accumulates primarily in the nuclei of H-position cells in the early meristematic region, and this localization requires the H-cell-expressed ENHANCER OF GLABRA3 (EGL3) basic helix-loop-helix transcription factor. These results suggest that cell-cell movement of CPC occurs readily within the meristematic region of the root and that EGL3 preferentially traps the CPC protein in the H-position cells of the epidermis.

show abstract

Section: Movement Of Cpc Protein Within and Between Tissues In The Desupporting

confidence: 74%

Nuclear Trapping Controls the Position-Dependent Localization of CAPRICE in the Root Epidermis of Arabidopsis

et al. 2013

View full text Add to dashboard Cite

show abstract

“…This contrasts with results for GFP-CPC in Arabidopsis, where movement between cells of the fusion protein was observed (Kurata et al, 2005;Digiuni et al, 2008). However, in that case, a specific amino acid motif was required for movement to occur, and a gated transport mechanism through plasmodesmata was suggested (Kurata et al, 2005;Wang et al, 2008;Rim et al, 2011). Notably, however, CPC variants lacking the motif were capable of intercellular movement in roots, suggesting passive diffusion as a secondary transport mechanism (Rim et al, 2011).…”

Section: Mybx Is a Repressormentioning

confidence: 71%

“…However, in that case, a specific amino acid motif was required for movement to occur, and a gated transport mechanism through plasmodesmata was suggested (Kurata et al, 2005;Wang et al, 2008;Rim et al, 2011). Notably, however, CPC variants lacking the motif were capable of intercellular movement in roots, suggesting passive diffusion as a secondary transport mechanism (Rim et al, 2011). An alternative interpretation of the biolistic data is that MYBx prevented AN11 from moving; however, this is unlikely given that the MYBs are not effective binders of WDR proteins (Figure 1; Payne et al, 2000;Baudry et al, 2004Baudry et al, , 2006Chiu et al, 2010;An et al, 2012).…”

Section: Mybx Is a Repressormentioning

confidence: 98%

A Conserved Network of Transcriptional Activators and Repressors Regulates Anthocyanin Pigmentation in Eudicots

et al. 2014

View full text Add to dashboard Cite

Plants require sophisticated regulatory mechanisms to ensure the degree of anthocyanin pigmentation is appropriate to myriad developmental and environmental signals. Central to this process are the activity of MYB-bHLH-WD repeat (MBW) complexes that regulate the transcription of anthocyanin genes. In this study, the gene regulatory network that regulates anthocyanin synthesis in petunia (Petunia hybrida) has been characterized. Genetic and molecular evidence show that the R2R3-MYB, MYB27, is an anthocyanin repressor that functions as part of the MBW complex and represses transcription through its C-terminal EAR motif. MYB27 targets both the anthocyanin pathway genes and basic-helix-loop-helix (bHLH) ANTHOCYANIN1 (AN1), itself an essential component of the MBW activation complex for pigmentation. Other features of the regulatory network identified include inhibition of AN1 activity by the competitive R3-MYB repressor MYBx and the activation of AN1, MYB27, and MYBx by the MBW activation complex, providing for both reinforcement and feedback regulation. We also demonstrate the intercellular movement of the WDR protein (AN11) and R3-repressor (MYBx), which may facilitate anthocyanin pigment pattern formation. The fundamental features of this regulatory network in the Asterid model of petunia are similar to those in the Rosid model of Arabidopsis thaliana and are thus likely to be widespread in the Eudicots.

show abstract

“…Given that the size exclusion limit of PD in the root meristem is suggested to be around 60 kD (Crawford and Zambryski, 2001;Rim et al, 2011), AHL4-GFP (72 kD) might move between cells in a targeted manner. To gain a better understanding of AHL4 movement, the coding region of AHL4 was fused with tandem yellow fluorescent proteins (YFPs; 3x or 4xYFP) driven by the CRE1 promoter and introduced into an ahl4-1 mutant.…”

Section: Movement Of Ahl4 Is Critical For the Boundary Between The Xymentioning

confidence: 99%

Cell-to-Cell Movement of Two Interacting AT-Hook Factors inArabidopsisRoot Vascular Tissue Patterning

et al. 2013

View full text Add to dashboard Cite

The xylem and phloem, major conducting and supporting tissues in vascular plants, are established by cell division and celltype specification in the procambium/cambium. The organization of the xylem, phloem, and procambium/cambium is tightly controlled. However, the underlying regulatory mechanisms remain largely unknown. In this study, we report the discovery of two transcription factors, AT-HOOK MOTIF NUCLEAR LOCALIZED PROTEIN 3 (AHL3) and AHL4, which regulate vascular tissue boundaries in Arabidopsis thaliana roots. In either of the knockout mutants of AHL3 and AHL4, encoding closely related AT-hook transcription factors, a misspecification of tissue boundaries between the xylem and procambium occurred and ectopic xylem developed in the procambium domain. In plants, specific types of transcription factors can serve as direct intercellular signals by moving from one cell to another, playing crucial roles in tissue patterning. Adding to this paradigm, AHL4 moves actively from the procambium to xylem in the root meristem to regulate the tissue boundaries. When the intercellular movement of AHL4 was impaired, AHL4 could not complement the xylem phenotype in the ahl4. Furthermore, AHL4 revealed unique characteristics in that it interacts with AHL3 in vivo and that this interaction facilitates their intercellular trafficking. Taken together, this study uncovered a novel mechanism in vascular tissue patterning that requires the intercellular trafficking of two interacting transcription factors.

show abstract

Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns

Cited by 51 publications

References 47 publications

Nuclear Trapping Controls the Position-Dependent Localization of CAPRICE in the Root Epidermis of Arabidopsis

Nuclear Trapping Controls the Position-Dependent Localization of CAPRICE in the Root Epidermis of Arabidopsis

A Conserved Network of Transcriptional Activators and Repressors Regulates Anthocyanin Pigmentation in Eudicots

Cell-to-Cell Movement of Two Interacting AT-Hook Factors inArabidopsisRoot Vascular Tissue Patterning

Contact Info

Product

Resources

About