Analysis of aquaporin 9 expression in human epidermis and cultured keratinocytes

Sugiyama, Yoshinori; Yamazaki, Kohei; Kusaka-Kikushima, Ayumi; Nakahigashi, Kyoko; Hagiwara, Hiromi

doi:10.1016/j.fob.2014.06.004

Cited by 14 publications

(16 citation statements)

References 34 publications

(51 reference statements)

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“…As regard AQP9, it was found in most part of keratinocytes granular layer where AQP3 expression was diminished [ 54 ]. Like AQP3, AQP9 is an aquaglyceroporin that plays a central role in glycerol metabolism, in cell motility and it could be an additional player in wound healing, but its function in skin has not yet been studied extensively.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Blue Light Induces Down-Regulation of Aquaporin 1, 3, and 9 in Human Keratinocytes

Avola

Graziano

Pannuzzo

et al. 2018

Cells

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The development in digital screen technology has exponentially increased in the last decades, and many of today’s electronic devices use light-emitting diode (LED) technology producing very strong blue light (BL) waves. Long-term exposure at LED-BL seems to have an implication in the dehydration of the epidermis, in the alterations of shape and number of the keratinocytes, and in the aging of the skin. Aquaporins (AQPs) are water membrane channels that permeate both water and glycerol and play an important role in the hydration of epidermis, as well as in proliferation and differentiation of keratinocytes. Thus, we have hypothesized that AQPs could be involved in the aging of the skin exposed to LED-BL. Therefore, we have examined the expression of AQPs in human keratinocytes exposed to LED-BL at dose of 45 J/cm2, used as an in vitro model to produce the general features of photo aging of the skin. The aim was to verify if LED-BL induces changes of the basal levels of AQPs. The keratinocytes exposure to LED-BL produced an increase of reactive oxygen species (ROS), an activation of 8-hydroxy-2’-deoxyguanosine (8-OHdG), an alteration of proliferating cell nuclear antigen (PCNA), and a down-regulation of AQP1, 3 and 9. These findings are preliminary evidences that may be used as starting points for further investigations about the mechanistic involvement of AQP1, 3, and 9 in LED-BL-induced skin aging.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: Blue Light Induces Down-Regulation of Aquaporin 1, 3, and 9 in Human Keratinocytes

Avola

Graziano

Pannuzzo

et al. 2018

Cells

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“…To date, 13 AQPs (AQP 0-12) have been cloned from humans and are categorized into distinct subgroups in terms of their amino acid sequence and molecular functions. (3) Aquaporin-3 (AQP3) as a water and glycerol channel is the key factor in the skin hydration mechanism as well as in keratinocyte proliferation and differentiation. (4,5) However, AQP3 expression in normal human epidermal keratinocytes (NHEKs) decreases along with the aging process.…”

Section: Original Article Introductionmentioning

confidence: 99%

Asiaticoside increases aquaporin-3 protein expression in the cytoplasm of normal human epidermal keratinocytes

Wijayadi

Darmawan

2017

UnivMed

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Introduction Skin hydration decreases with aging. Aquaporin-3 (AQP3) is a major protein that plays a role in skin hydration, therefore it is a novel target for skin moisturizing treatment. Retinoic acid (RA) as a well-known active agent in antiaging treatment increases AQP3 expression, but frequently causes harmful side effects. Asiaticoside, a saponin compound isolated from Centella asiatica (CA) is also known as an antiaging cosmetic and plays a role in wound healing. The aim of this study was to evaluate and compare the effect of asiaticoside isolated from CA and the effect of RA on the AQP3 expression in normal human epidermal keratinocytes (NHEKs).Methods An experimental laboratory study was performed using primary NHEKs that were derived from the foreskin of a boy. AQP3 expression in NHEKs was examined in vitro after the cells were incubated for 24 hours with asiaticoside or with RA at several concentrations. The AQP3 expression was evaluated by immunocytochemistry and quantitatively analyzed by Image-J software. Independent t-test and one-way ANOVA were used to analyze the data, followed by post-hoc Tukey test.Results There was an increasing trend of AQP3 expression upon exposure to asiaticoside at all concentrations compared to the control group. However, RA exposure seemed to induce a higher level of AQP3 expression. Asiaticoside effected a lower increase in AQP3 expression in NHEKs than did RA (p=0.042). Optimal results were achieved at 1 mg/mL concentration of asiaticoside.Conclusions Asiaticoside isolated from CA can enhance the AQP3 expression in NHEKs. Therefore it can be used as an active ingredient in cosmetic moisturizer formulation for dry skin treatment.

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“…In these original reports, AQP9 mRNA expression was found in a few tissues, most notably liver and leukocytes in humans (Ishibashi et al 1998;Tsukaguchi et al 1999), as well as liver and immature sperm in mice (Tsukaguchi et al 1998). Since then, AQP9 immunoreactivity has been found in many tissues, including rat and human epididymis (Elkjaer et al 2000;Pastor-Soler et al 2001), various cell types of rodent and primate brain (Elkjaer et al 2000;Badaut et al 2001;Badaut et al 2004;Amiry-Moghaddam et al 2005;Arcienega et al 2010), mouse spinal cord (Oshio et al 2004), human chorioamnion and placenta (Damiano et al 2001;Wang et al 2004), mouse and human inner ear (Huang et al 2002;Degerman et al 2011), mouse and human small intestine (Okada et al 2003), rat and human prostate (Wang et al 2008;Hwang et al 2012), human skeletal muscle (Inoue et al 2009), urothelium (Rubenwolf et al 2009), rat and porcine oviduct (Skowronski et al 2009), human adipose tissue (Rodriguez et al 2011), human retina (Tran et al 2013;Yang et al 2013), and mouse and human skin (Sugiyama et al 2014). …”

Section: Introductionmentioning

confidence: 99%

A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues

Lindskog

Asplund

Catrina

et al. 2016

J Histochem Cytochem.

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SummaryAQP9 is known to facilitate hepatocyte glycerol uptake. Murine AQP9 protein expression has been verified in liver, skin, epididymis, epidermis and neuronal cells using knockout mice. Further expression sites have been reported in humans. We aimed to verify AQP9 expression in a large set of human normal organs, different cancer types, rheumatoid arthritis synovial biopsies as well as in cell lines and primary cells. Combining standardized immunohistochemistry with high-throughput mRNA sequencing, we found that AQP9 expression in normal tissues was limited, with high membranous expression only in hepatocytes. In cancer tissues, AQP9 expression was mainly found in hepatocellular carcinomas, suggesting no general contribution of AQP9 to carcinogenesis. AQP9 expression in a subset of rheumatoid arthritis synovial tissue samples was affected by Humira, thereby supporting a suggested role of TNFα in AQP9 regulation in this disease. Among cell lines and primary cells, LP-1 myeloma cells expressed high levels of AQP9, whereas low expression was observed in a few other lymphoid cell lines. AQP9 mRNA and protein expression was absent in HepG2 hepatocellular carcinoma cells. Overall, AQP9 expression in human tissues appears to be more selective than in mice. (J Histochem Cytochem 64:287-300, 2016)

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Analysis of aquaporin 9 expression in human epidermis and cultured keratinocytes

Abstract: HighlightsAQP9 is expressed in the upper layer of the stratum granulosum of human epidermis.AQP9 expressed in keratinocytes facilitates glycerol and urea transportation.Retinoic acid down-regulates AQP9 mRNA expression in differentiated keratinocytes.

Cited by 14 publications

References 34 publications

RETRACTED: Blue Light Induces Down-Regulation of Aquaporin 1, 3, and 9 in Human Keratinocytes

RETRACTED: Blue Light Induces Down-Regulation of Aquaporin 1, 3, and 9 in Human Keratinocytes

Asiaticoside increases aquaporin-3 protein expression in the cytoplasm of normal human epidermal keratinocytes

A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues

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