Analysis of Airborne Biomarkers for Point-of-Care Diagnostics

Fung, Andrew O.; Mykhaylova, Natalia

doi:10.1177/2211068213517119

Cited by 16 publications

(14 citation statements)

References 200 publications

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“…The application of these e-nose solutions to large populations is much easier than a comprehensive study using MS detection and certainly the data obtained would be very relevant. Nevertheless, there are a number of issues that have to be solved before e-noses can reach a level to be used in medical diagnosis (reviewed in [ 7 , 152 , 153 ]).…”

Section: Exhaled Breath (Eb) Analysismentioning

confidence: 99%

Breath Analysis as a Potential and Non-Invasive Frontier in Disease Diagnosis: An Overview

et al. 2015

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Currently, a small number of diseases, particularly cardiovascular (CVDs), oncologic (ODs), neurodegenerative (NDDs), chronic respiratory diseases, as well as diabetes, form a severe burden to most of the countries worldwide. Hence, there is an urgent need for development of efficient diagnostic tools, particularly those enabling reliable detection of diseases, at their early stages, preferably using non-invasive approaches. Breath analysis is a non-invasive approach relying only on the characterisation of volatile composition of the exhaled breath (EB) that in turn reflects the volatile composition of the bloodstream and airways and therefore the status and condition of the whole organism metabolism. Advanced sampling procedures (solid-phase and needle traps microextraction) coupled with modern analytical technologies (proton transfer reaction mass spectrometry, selected ion flow tube mass spectrometry, ion mobility spectrometry, e-noses, etc.) allow the characterisation of EB composition to an unprecedented level. However, a key challenge in EB analysis is the proper statistical analysis and interpretation of the large and heterogeneous datasets obtained from EB research. There is no standard statistical framework/protocol yet available in literature that can be used for EB data analysis towards discovery of biomarkers for use in a typical clinical setup. Nevertheless, EB analysis has immense potential towards development of biomarkers for the early disease diagnosis of diseases.

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Section: Exhaled Breath (Eb) Analysismentioning

confidence: 99%

Breath Analysis as a Potential and Non-Invasive Frontier in Disease Diagnosis: An Overview

et al. 2015

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“…In particular, there is an outstanding need to monitor pathogens in air in critical infrastructure such as government buildings, hospitals, mass transit, and airports, during manufacturing in clean rooms and ‘ready-to-eat’ food preparation, to name but a few. There is also a growing interest in detection of infectious bioaerosols for point-of-care diagnostics ( Fung and Mykhaylova, 2014 ). Most air samplers operate by filtration, electrostatic precipitation, or inertial/gravitational collection ( Fronczek and Yoon, 2015 ; Sharma Ghimire et al , 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling

Sousa

Shen

Silcott³

et al. 2020

Annals of Work Exposures and Health

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Detecting infectious aerosols is central for gauging and countering airborne threats. In this regard, the Coriolis® µ cyclonic air sampler is a practical, commercial collector that can be used with various analysis methods to monitor pathogens in air. However, information on how to operate this unit under optimal sampling and biosafety conditions is limited. We investigated Coriolis performance in aerosol dispersal experiments with polystyrene microspheres and Bacillus globigii spores. We report inconsistent sample recovery from the collector cone due to loss of material when sampling continuously for more than 30 min. Introducing a new collector cone every 10 min improved this shortcoming. Moreover, we found that several surfaces on the device become contaminated during sampling. Adapting a high efficiency particulate air-filter system to the Coriolis prevented contamination without altering collection efficiency or tactical deployment. A Coriolis modified with these operative and technical improvements was used to collect aerosols carrying microspheres released inside a Biosafety Level-3 laboratory during simulations of microbiological spills and aerosol dispersals. In summary, we provide operative and technical solutions to the Coriolis that optimize microbiological air sampling and improve biosafety.

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“…To overcome challenges associated with bioaerosol exposure studies, the aerosol science community has identified the need to explore new alternatives for assessing bioaerosol exposure. For example, the identification of markers associated with exposure could help ascertain which environments pose the highest risks (Fung and Mikhaylova 2014;Garcia-Alcega et al 2017). Recent studies have identified a strong correlation between the bacterial community of the upper respiratory tract (nasal cavity and nasopharynx) of exposed subjects and the bioaerosols collected from the environment they were exposed to, using an HTS approach (Mbareche et al, manuscript in preparation;Kraemer et al 2018).…”

Section: Environmental Exposure Markersmentioning

confidence: 99%

On the interpretation of bioaerosol exposure measurements and impacts on health

Mbareche

Morawska

Duchaine

2019

Journal of the Air & Waste Management Association

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Bioaerosols are recognized as one of the main transmission routes for infectious diseases and are responsible for other various types of health effects through inhalation and potential ingestion. Associating exposure with bioaerosol and health problems is challenging, and adequate exposure monitoring is a top priority for aerosol scientists. The multiple factors affecting bioaerosol content, the variability in the focus of each bioaerosol exposure study, and the variations in experimental design and the standardization of methods make bioaerosol exposure studies very difficult. Therefore, the health impacts of bioaerosol exposure are still poorly understood. This paper presents a brief description of a state-of-the-art development in bioaerosol exposure studies supported by studies on several related subjects. The main objective of this paper is to propose new considerations for bioaerosol exposure guidelines and the development of tools and study designs to better interpret bioaerosol data. The principal observations and findings are the discrepancy of the applicable methods in bioaerosol studies that makes result comparison impossible. Furthermore, the silo mentality helps in creating a bigger gap in the knowledge accumulated about bioaerosol exposure. Innovative and original ideas are presented for aerosol scientists and health scientists to consider and discuss. Although many examples cited herein are from occupational exposure, the discussion has relevance to any human environment. This work gives concrete suggestions for how to design a full bioaerosol study that includes all of the key elements necessary to help understand the real impacts of bioaerosol exposure in the short term. The creation of the proposed bioaerosol public database could give crucial information to control the public health.Implications: How can we move toward a bioaerosol exposure guidelines? The creation of the bioaerosol public database will help accumulate information for long-term association studies and help determine specific exposure biomarkers to bioaerosols. The implementation of such work will lead to a deeper understanding and more efficient utilization of bioaerosol studies to prevent public health hazards. PAPER HISTORY

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Analysis of Airborne Biomarkers for Point-of-Care Diagnostics

Cited by 16 publications

References 200 publications

Breath Analysis as a Potential and Non-Invasive Frontier in Disease Diagnosis: An Overview

Breath Analysis as a Potential and Non-Invasive Frontier in Disease Diagnosis: An Overview

Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling

On the interpretation of bioaerosol exposure measurements and impacts on health

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