On the interpretation of bioaerosol exposure measurements and impacts on health

Mbareche, Hamza; Morawska, Lidia; Duchaine, Caroline

doi:10.1080/10962247.2019.1587552

Cited by 52 publications

(38 citation statements)

References 142 publications

(137 reference statements)

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“…The presence of dynamic and highly diversified microbial communities in the air of urban and rural environments assumes a major impact on Public Health due to their dispersion and health consequences [32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Contamination in Health Care Centers: Differences between Urban and Rural Settings

et al. 2021

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This study aims to assess the airborne bioburden of rural and urban Portuguese Primary Health Care Centers (PHCC) using active and passive sampling methods and identify the potential differences in airborne microbiota between both environments. The highest total aerobic mesophilic bacterial load in indoor air were found in the Vaccination Room (448 CFU.m−3) in the Rural PHCC and in the Waiting Room (420 CFU.m−3) for Urban PHCC. The total coliforms contamination level in indoor air was detected only in the Cleaning Supplies Room (4 CFU.m−3) in the Urban PHCC. The most frequent bacteria genera identified was Micrococcus (21% Rural PHCC; 31% Urban PHCC). The surface samples showed a highest total aerobic mesophilic bacterial contamination in the Treatment Room (86 × 103 CFU.m−2) from the Rural PHCC and in the Front Office (200 × 103 CFU.m−2) from the Urban PHCC. The electrostatic dust cloth (EDC) samples showed a highest bacterial load in the Urban PHCC. Total aerobic mesophilic bacterial load in settled dust and in the Heating, Ventilating and Air Conditioning (HVAC) filter samples in the Urban PHCC (8 CFU.g−1 and 6 × 103 CFU.m−2) presented higher values compared with the Rural PHCC (1 CFU.g−1 and 2.5 × 103 CFU.m−2). Urban PHCC presented higher bacterial airborne contamination compared with the Rural PHCC for the majority of sampling sites, and when compared with the indoor air quality (IAQ) Portuguese legislation it was the Rural PHCC in two sampling places who did not comply with the established criteria.

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Section: Introductionmentioning

confidence: 99%

Bacterial Contamination in Health Care Centers: Differences between Urban and Rural Settings

et al. 2021

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“…This is a novel method, on the other hand the extraction procedure from filters probably have to be optimized. The application of biomolecular methods allows a better characterization of the bioaerosol in bioenergy plants showing that in such environment the contamination level, suggested as occupation limits in several country from 10 3 to 10 4 UFC/m 3 , are often reached [15]. This supports the necessity of an accurate biological risk assessment for each worker profile.…”

Section: Resultsmentioning

confidence: 99%

“…A risk assessment’s critical point process is the characterization step where the bioaerosol quality and quantity are crucial data to correlate to human health damage [2,13,14]. There is still a lack of evidence on the human dose/response relationship and on standard protocols to adopt for bioaerosol sampling and analysis [15]. The increase and heterogeneity of the biomasses involved in biofuel production make the biological risk assessment a priority in the plants.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of Air Microbial Contaminations in Different Bioenergy Facilities Using Cultural and Biomolecular Methods

Anedda¹,

Carletto²,

Gilli³

et al. 2019

IJERPH

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Bioaerosol exposure linked to the bioenergy production from waste and its effects on human health in occupational and residential environments has rising interest nowadays. The health risk associated with the exposure includes mainly infective diseases, allergies, chronic bronchitis, and obstructive pulmonary disease. A risk assessment’s critical point is the bioaerosol quality and quantity characterization. The aim of this study is to evaluate the application of different methods for the analysis of bioaerosol sampled in bioenergy plants. This study involved six Italian plants for the treatment of biomasses and energy production. Bioaerosol cultural evaluation was performed, by Surface Air System (SAS) sampler, and DNA was extracted from PM0.49 samples and Low Melting Agar plates. qRT-PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE) and band sequencings were performed. The cultural method is able to detect less than 15% of what is evaluable with bio-molecular methods. A low sample alfa-diversity and a high beta-biodiversity in relation to feedstock and process were observed. Sequencing showed microorganisms with a hygienic-sanitary relevance such as Arcobacter, Pseudomonas, Enterobacter, Klebsiella, Enterococcus and Bacillus. Integrated cultural and biomolecular methods can be more exhaustive to evaluate bioaerosol’s exposure in the occupational environment.

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“…It supports the idea of creating a worldwide database, that lists potential markers specific to certain environments, and to the nasopharynxes of the people working in them. This database could represent an important asset for associating bioaerosol exposure with health problems [91].…”

Section: Discussionmentioning

confidence: 99%

Bioaerosols Play a Major Role in the Nasopharyngeal Microbiota Content in Agricultural Environment

Mbareche

Veillette

Pilote

et al. 2019

IJERPH

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Background: Bioaerosols are a major concern for public health and sampling for exposure assessment purposes is challenging. The nasopharyngeal region could be a potent carrier of long-term bioaerosol exposure agents. This study aimed to evaluate the correlation between nasopharyngeal bacterial flora of swine workers and the swine barns bioaerosol biodiversity. Methods: Air samples from eight swine barns as well as nasopharyngeal swabs from pig workers (n = 25) and from a non-exposed control group (n = 29) were sequenced using 16S rRNA gene high-throughput sequencing. Wastewater treatment plants were used as the industrial, low-dust, non-agricultural environment control to validate the microbial link between the bioaerosol content (air) and the nasopharynxes of workers. Results: A multivariate analysis showed air samples and nasopharyngeal flora of pig workers cluster together, compared to the non-exposed control group. The significance was confirmed with the PERMANOVA statistical test (p-value of 0.0001). Unlike the farm environment, nasopharynx samples from wastewater workers did not cluster with air samples from wastewater treatment plants. The difference in the microbial community of nasopharynx of swine workers and a control group suggest that swine workers are carriers of germs found in bioaerosols. Conclusion: Nasopharynx sampling and microbiota could be used as a proxy of air sampling for exposure assessment studies or for the determination of exposure markers in highly contaminated agricultural environments.

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On the interpretation of bioaerosol exposure measurements and impacts on health

Cited by 52 publications

References 142 publications

Bacterial Contamination in Health Care Centers: Differences between Urban and Rural Settings

Bacterial Contamination in Health Care Centers: Differences between Urban and Rural Settings

Monitoring of Air Microbial Contaminations in Different Bioenergy Facilities Using Cultural and Biomolecular Methods

Bioaerosols Play a Major Role in the Nasopharyngeal Microbiota Content in Agricultural Environment

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