Analysis of adipose tissues and stromal vascular cells in a murine arthritis model

Hamaguchi, Kanae; Itabashi, Ayako; Kuroe, Yuka; Nakano, Mana; Fujimoto, Eka; Kato, Tomomi; Satoi, Keiko; Utsuyama, Masanori; Sato, Kazuto

doi:10.1016/j.metabol.2012.05.018

Cited by 13 publications

(8 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a study related to high‐fat diet in CIA, the authors have shown increased lipolysis related to CIA development, higher IL‐17 production, and no difference in animal weight compared with CIA fed with normal diet . Furthermore, in adjuvant‐induced arthritis animal model, several authors have found decreased fat mass compared with healthy controls . Martín et al .…”

Section: Discussionmentioning

confidence: 99%

“…34 Furthermore, in adjuvant-induced arthritis animal model, several authors have found decreased fat mass compared with healthy controls. [35][36][37] Martín et al 35 suggests that the mechanism is due to a decrease in white adipose mass is secondary to a reduced adipose lipogenesis. Lopez-Menduina et al 37 have found decreased body weight gain in adjuvant-induced arthritis rats, suggesting an inhibitory effect of inflammation on body weight, in which diseased animals has lower relative fat mass compared with pair-fed healthy rats.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Collagen‐induced arthritis as an animal model of rheumatoid cachexia

Alabarse

Lora

Silva

et al. 2018

J cachexia sarcopenia muscle

View full text Add to dashboard Cite

BackgroundRheumatoid arthritis is characterized by chronic polyarticular synovitis and presents systemic changes that impact quality of life, such as impaired muscle function, seen in up to 66% of the patients. This can progress to severely debilitating state known as rheumatoid cachexia—without loss of fat mass and body weight—for which there is little consensus in terms of diagnosis or treatment. This study aims to evaluate whether the collagen‐induced arthritis (CIA) animal model also develops clinical and functional features characteristic of rheumatoid cachexia.MethodsMale DBA1/J mice were randomly divided into 2 groups: healthy animals (CO, n = 11) and CIA animals (n = 13). The clinical score and edema size, animal weight and food intake, free exploratory locomotion, grip strength, and endurance exercise performance were tested 0, 18, 35, 45, 55, and 65 days after disease induction. After euthanasia, several organs, visceral and brown fat, and muscles were dissected and weighed. Muscles were used to assess myofiber diameter. Ankle joint was used to assess arthritis severity by histological score. Statistical analysis were performed using one‐way and two‐way analyses of variance followed by Tukey's and Bonferroni's test or t‐test of Pearson and statistical difference were assumed for a P value under 0.05.ResultsThe CIA had significantly higher arthritis scores and larger hind paw edema volumes than CO. The CIA had decreased endurance exercise performance total time (fatigue; 23, 22, 24, and 21% at 35, 45, 55, and 65 days, respectively), grip strength (27, 55, 63, 60, and 66% at 25, 35, 45, 55, and 65 days, respectively), free locomotion (43, 57, 59, and 66% at 35, 45, 55, and 65 days, respectively), and tibialis anterior and gastrocnemius muscle weight (25 and 24%, respectively) compared with CO. Sarcoplasmic ratios were also reduced in CIA (TA: 23 and GA: 22% less sarcoplasmic ratio), confirming the atrophy of skeletal muscle mass in these animals than in CO. Myofiber diameter was also reduced 45% in TA and 41% in GA in CIA when compared with the CO. Visceral and brown fat were lighter in CIA (54 and 39%, respectively) than CO group.ConclusionsThe CIA model is a valid experimental model for rheumatoid cachexia given that the clinical changes observed were similar to those described in patients with rheumatoid arthritis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Collagen‐induced arthritis as an animal model of rheumatoid cachexia

Alabarse

Lora

Silva

et al. 2018

J cachexia sarcopenia muscle

View full text Add to dashboard Cite

show abstract

“…A link between the neuroendocrine and immune systems has been shown to contribute to the pathogenesis of several immune mediated inflammatory disorders (Cassano et al, 2014;Procaccini et al, 2014). In this context adipo-cytokines, such as leptin and adiponectin, hormones secreted mainly by the adipose tissue, have been shown to play a role in RA pathogenesis (Hamaguchi et al, 2012;Ruscitti et al, 2018). For instance, it has been shown that ob/ob mice develop resistance to experimental antigen-induced arthritis compared to wild-type mice (Busso et al, 2002).…”

Section: Adipo-cytokinesmentioning

confidence: 99%

Metabolic Checkpoints in Rheumatoid Arthritis

et al. 2020

View full text Add to dashboard Cite

Several studies have highlighted the interplay between metabolism, immunity and inflammation. Both tissue resident and infiltrating immune cells play a major role in the inflammatory process of rheumatoid arthritis (RA) via the production of cytokines, adipocytokines and metabolic intermediates. These functions are metabolically demanding and require the most efficient use of bioenergetic pathways. The synovial membrane is the primary site of inflammation in RA and exhibits distinctive histological patterns characterized by different metabolism, prognosis and response to treatment. In the RA synovium, the high energy demand by stromal and infiltrating immune cells, causes the accumulation of metabolites, and adipo-cytokines, which carry out signaling functions, as well as activating transcription factors which act as metabolic sensors. These events drive immune and joint-resident cells to acquire pro-inflammatory effector functions which in turn perpetuate chronic inflammation. Whether metabolic changes are a consequence of the disease or one of the causes of RA pathogenesis is still under investigation. This review covers our current knowledge of cell metabolism in RA. Understanding the intricate interactions between metabolic pathways and the inflammatory and immune responses will provide more awareness of the mechanisms underlying RA pathogenesis and will identify novel therapeutic options to treat this disease.

show abstract

“…Leptin comes mainly from adipose cells. Most studies have found significantly elevated serum levels of leptin in RA patients [7,18,22,[26][27][28]30,36,40,92,93], while others have found decreased levels [19,21,32,34]. Although the relationship is complex, leptin has been shown to be the major factor linkage of food intake with bone metabolism [94].…”

Section: Leptin and Rheumatoid Arthritismentioning

confidence: 99%

Emerging role of leptin in rheumatoid arthritis

Tian

Liang

Wang

et al. 2014

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryNumerous studies have suggested the importance of leptin against autoimmune diseases such as systemic lupus erythematosus (SLE), multiple sclerosis (MS) and psoriasis. To summarize our current understanding of the role of leptin in inflammatory responses and rheumatoid arthritis (RA), a systematic review was conducted to assess the discrepancy of leptin in RA and its effect on immunity according to different studies. Recently, emerging data have indicated that leptin is involved in the pathological function of RA, which is common in autoimmune disorders. This review discusses the possible consequences of leptin levels in RA. Blocking the key signal pathways of leptin and inhibiting the leptin activity-like leptin antagonist may be a promising way for potential therapeutic treatment of RA at risk of detrimental effects. However, leptin was increased in patients with RA and may also regulate joint damage. Thus, more understanding of the mechanism of leptin in RA would be advantageous in the future.

show abstract

Analysis of adipose tissues and stromal vascular cells in a murine arthritis model

Cited by 13 publications

References 39 publications

Collagen‐induced arthritis as an animal model of rheumatoid cachexia

Collagen‐induced arthritis as an animal model of rheumatoid cachexia

Metabolic Checkpoints in Rheumatoid Arthritis

Emerging role of leptin in rheumatoid arthritis

Contact Info

Product

Resources

About