Analysis of Adipose-Derived Stem Cells from Different Donor Areas and Their Influence on Fibroblasts In Vitro

Zampar, Antonio Gustavo; Farina, Jayme Adriano; Orellana, Maristela Delgado; Caruso, Sâmia Rigotto; Fernandes, Taisa Risque; Gomes, Rogério; Aragon, Davi Casale; Santis, Gil Cunha De; Covas, Dimas Tadeu

doi:10.1007/s00266-019-01586-0

Cited by 8 publications

(4 citation statements)

References 14 publications

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“…Even though ADMSCs are well-known as multipotent stem cells, different factors such as donor age, cell culture conditions, and anatomical position of extracted adipose tissue may affect the rate of proliferation and phenotype of the ADSCs and their multipotent differentiation potential. − Therefore, to evaluate the multipotency potential of extracted MSCs for generation of different linages such as osteogenic, adipogenic, and chondrogenic lines, cells were harvested at passage 3 with trypsin–EDTA (0.25%) and cultured on a 12-well plate.…”

Section: Methodsmentioning

confidence: 99%

Mineralized Human Amniotic Membrane as a Biomimetic Scaffold for Hard Tissue Engineering Applications

Sabouri

Farzin

Kabiri

et al. 2020

ACS Biomater. Sci. Eng.

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The human amniotic membrane (HAM) has been viewed as a potential regenerative material for a wide variety of injured tissues because of its collagen-rich content. High degradability of HAM limits its wide practical application in bone tissue engineering. In this study, the natural matrix of the decellularized amniotic membrane was developed by the double diffusion method. The results confirmed a reduction of the amniotic membrane’s degradability because of the deposition of calcium and phosphate ions during the double diffusion process. Real-time PCR results showed a high expression of osteogenesis-related genes from adipose-derived mesenchymal stem cells (ADMSCs) cultured on the surface of the developed mineralized amniotic membrane (MAM). Further in vivo experiments were conducted using an MAM preseeded with ADMSCs and a critical-size rat calvarial defect model. Histopathological results confirmed that the MAM + cell sample has excellent potential in bone regeneration.

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Section: Methodsmentioning

confidence: 99%

Mineralized Human Amniotic Membrane as a Biomimetic Scaffold for Hard Tissue Engineering Applications

Sabouri

Farzin

Kabiri

et al. 2020

ACS Biomater. Sci. Eng.

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“…Zampar et al analyzed AD-MSCs from different donor areas: upper abdomen, lower abdomen, tights, dorsum, and flanks [ 24 ]. They investigated differences in the AD-MSCs by analyzing the action of the supernatant produced by AD-MSCs from different body areas on fibroblast migration, and differences in the secretome present in the supernatant produced by these cells.…”

Section: Differentiating Potentialmentioning

confidence: 99%

“…The biological material was collected by liposuction. Their work showed that the dorsum provided the highest concentration of AD-MSCs than the other areas of the body [ 24 ]. Analysis of the secretomes showed a significant difference in VEGF (vascular endothelial growth factor) concentrations, which were lower in the control group.…”

Section: Differentiating Potentialmentioning

confidence: 99%

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Adipose-Derived Mesenchymal Stromal Cells in Basic Research and Clinical Applications

Czerwiec

Zawrzykraj

Deptuła

et al. 2023

IJMS

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Adipose-derived mesenchymal stromal cells (AD-MSCs) have been extensively studied in recent years. Their attractiveness is due to the ease of obtaining clinical material (fat tissue, lipoaspirate) and the relatively large number of AD-MSCs present in adipose tissue. In addition, AD-MSCs possess a high regenerative potential and immunomodulatory activities. Therefore, AD-MSCs have great potential in stem cell-based therapies in wound healing as well as in orthopedic, cardiovascular, or autoimmune diseases. There are many ongoing clinical trials on AD-MSC and in many cases their effectiveness has been proven. In this article, we present current knowledge about AD-MSCs based on our experience and other authors. We also demonstrate the application of AD-MSCs in selected pre-clinical models and clinical studies. Adipose-derived stromal cells can also be the pillar of the next generation of stem cells that will be chemically or genetically modified. Despite much research on these cells, there are still important and interesting areas to explore.

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Effects of Harvest Sites on Cryopreserved Adipose-Derived Stem Cells and ASC-Enriched Fat Grafts

Wang

et al. 2020

Aesth Plast Surg

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Analysis of Adipose-Derived Stem Cells from Different Donor Areas and Their Influence on Fibroblasts In Vitro

Cited by 8 publications

References 14 publications

Mineralized Human Amniotic Membrane as a Biomimetic Scaffold for Hard Tissue Engineering Applications

Mineralized Human Amniotic Membrane as a Biomimetic Scaffold for Hard Tissue Engineering Applications

Adipose-Derived Mesenchymal Stromal Cells in Basic Research and Clinical Applications

Effects of Harvest Sites on Cryopreserved Adipose-Derived Stem Cells and ASC-Enriched Fat Grafts

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