Analysis of a polymorphism in the DGAT1 gene in 14 cattle breeds through PCR-SSCP methods

Ripoli, María Verónica; Corva, P. M.; Giovambattista, Guillermo

doi:10.1016/j.rvsc.2005.07.006

Cited by 23 publications

(19 citation statements)

References 18 publications

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“…Since this band was not seen in the BB genotype and since sequencing of the PCR products of the AA genotype revealed no discrepancy between the sequences of different samples or the forward and reverse sequences, we concluded that the additional band was an alternative form of the allele A DNA sequence. This conclusion agrees with the demonstration that strands with the same sequence may yield different stable conformations known as isoconformers (Ripoli et al , 2006; Chessa et al , 2010). …”

supporting

confidence: 91%

Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP

Raut

Kumar²,

Kala

et al. 2012

Genet. Mol. Biol.

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Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may influence DGAT1 expression and function. The results described here could be useful in examining the association between the DGAT1 gene and milk traits in buffalo.

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supporting

confidence: 91%

Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP

Raut

Kumar²,

Kala

et al. 2012

Genet. Mol. Biol.

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“…Las frecuencias halladas en la reserva son muy similares a las reportadas previamente para las razas españolas Toro de Lidia y Asturiana de la Montaña (Kaupe et al, 2004). Otros autores encuentran frecuencias más equilibradas para ambos alelos en poblaciones de bovinos Criollos de Argentina y Bolivia, siendo una de las posibles causas la introgresión de Bos indicus (Lirón et al, 2002;Ripoli et al, 2006). Si bien puede existir un bajo nivel de introgresión de razas cebuinas en las poblaciones cruza analizadas aquí, su influencia en los resultados obtenidos no es evidente.…”

Section: Discussionunclassified

Marcadores moleculares asociados al veteado de la carne en bovinos Criollos uruguayos

et al. 2011

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“…Literature may also report that the "K" allele is responsible from the saturated fat acids in the milk [3] . The individual properties of the related loci of the cattle races can affect the DGAT1 K232A polymorphism and this may produce different effects on milk fat [4,5] . The utility of the cattles for milk and meat production, their adaptation to natural conditions and tolerance to diseases makes them economically advantageous [6,7] .…”

Section: Comparision Of Dgat1 K232a Polymorphism and Its Effects On Smentioning

confidence: 99%

“…In order to pick up the K232A polymorphism, PCR -SSCP (Polymerase Chain Reaction -Single Strand Conformation Polymorphism) procedure has been applied according to Ripoli's [4] method. PCR amplification has been applied in 26 µl total volume.…”

Section: Determination Of Dgat1 Genementioning

confidence: 99%

Comparision of DGAT1 K232A Polymorphism and its Effects on some Milk Quality Parameters in Holstein and Native Black Race Cattles

Cerit¹,

Dümen²,

Sezgin³

2014

Kafkas Univ Vet Fak Derg

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SummaryIn this study, effects of DGAT1 K232 polymorphism and the allele frequency differences of "K" (alanine variant) and "L" (lysine variant) on some cattle milk qualities and some microbiological parameters which pose a risk on consumer health were investigated. For this purpose, 2 years old 50 cattles were used as material (25 Holstein race and 25 Native Black race). Blood samples were collected for determining the allele frequencies of DGAT1 gene. Then, during the experimental period (1 month) the milk of the cattles were collected once a week and the milk samples has been explored for some important foodborne pathogens (Total Aerobic Bacteria, Coliforms, Escherichia coli, Listeria monocytogenes and Staphylococcus aureus) and milk quality parameters (pH, fat, density and acidity). According to the findings, it has been identified that DGAT1 gene frequency differences were significantly effective on some the parameters of milk qualities and the growth of coliforms, Esherichia coli and Staphylococcus aureus. Keywords: DGAT1 gene, Milk quality, Polymorphism, Cattle, Foodborne pathogens Holstein ve Yerli Kara Sığır Irklarında DGAT1 K232a Poliformizminin ve Bunların Bazı Süt Kalite Parametrelerine Etkisinin Araştırılması ÖzetBu çalışmada, farklı ırk sığırlarda DGAT1 K232 polimorfizmi araştırılmış ve DGAT1 geninde bulunan "K" (alanin varyantı) ve "L" (lizin vasryantı) allel frekans farklılıklarının sığırların bazı süt kalite (süt verimi, sütte yağ oranı, pH vb.) parametrelerine ve tüketici sağlığı açısından risk teşkil eden önemli bazı gıda kaynaklı patojenlere etkisi incelenmiştir. Bu amaçla 2 yaşlı, 50 adet sığır (25 adet Holstein ırkı, 25 adet ise Yerlikara ırkı) materyal olarak kullanılmıştır. Sığırlardan kan örnekleri alınarak DGAT1 geni allel frekansları belirlenmiş ve daha sonraki dönemde ise haftada 1 kez olmak üzere 1 ay boyunca aynı sığırlardan süt örnekleri toplanmış ve toplanan süt örnekleri bazı önemli gıda kaynaklı mikrobiyolojik parametreler (toplam mezofilik erobik bakteri, koliformlar, Escherichia coli, Listeria monocytogenes ve Staphylococcus aureus olmak üzere) ve süt kalite parametreleri (pH, süt yağı, sütün yoğunluğu ve sütün asiditesi olmak üzere) açısından incelenmiştir. Elde edilen bulgulara göre DGAT1 geni allel frekans farklılıklarının, tüm süt kalite parametreleri ve mikrobiyolojik parametrelerden koliformlar, Esherichia coli ve Staphylococcus aureus üremelerinin üzerine istatistik açıdan belirgin derecede etkili olduğu tespit edilmiştir.

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Analysis of a polymorphism in the DGAT1 gene in 14 cattle breeds through PCR-SSCP methods

Cited by 23 publications

References 18 publications

Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP

Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP

Marcadores moleculares asociados al veteado de la carne en bovinos Criollos uruguayos

Comparision of DGAT1 K232A Polymorphism and its Effects on some Milk Quality Parameters in Holstein and Native Black Race Cattles

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