Analysis of a Brazilian green propolis from Baccharis dracunculifolia by HPLC-APCI-MS and GC-MS

Many biological properties have been attributed to various types of propolis, including anti-inflammatory, antimicrobial, antioxidant, antitumor, wound healing, and immunomodulatory activities. This article reviewed studies published that investigated the anti-inflammatory activity of propolis of different origins and/ or its isolated components, focusing on the mechanisms of action underlying this activity and also addressing some aspects of immunomodulatory effects. The search was performed of the following databases: PubMed, Science Direct, HighWire Press, Scielo, Google Academics, Research Gate and ISI Web of Knowledgement. The anti-inflammatory activity was associated with propolis or compounds such as polyphenols (flavonoids, phenolic acids and their esters), terpenoids, steroids and amino acids. CAPE is the most studied compounds. The main mechanisms underlying the anti-inflammatory activity of propolis included the inhibition of cyclooxygenase and consequent inhibition of prostaglandin biosynthesis, free radical scavenging, inhibition of nitric oxide synthesis, reduction in the concentration of inflammatory cytokines and immunosuppressive activity. Propolis was found to exert an antiinflammatory activity in vivo and in vitro models of acute and chronic inflammation and others studies, indicating its promising potential as anti-inflammatory agent of natural origin and as a source of chemical compounds for the development of new drugs.

Section: Introductionmentioning

confidence: 99%

Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propolis: a brief review

Araújo

Libério

Guerra

et al. 2012

“…A própolis é considerada uma das misturas mais heterogêneas encontradas em fontes naturais, sendo que mais de 300 constituintes já foram identificados e/ou caracterizados em diferentes amostras (Burdock, 1998;Chang et al, 2008;Lustosa et al, 2008;Sousa et al, 2008). As abelhas (Apis mellifera) usam esta substância para protegê-las contra insetos e microorganismos, empregando-a em finas camadas nas paredes internas das colméias para vedar buracos e rachaduras, reparar e fortalecer os favos de mel, proteger a entrada da colméia, no preparo de locais assépticos para a postura da abelha rainha e na mumificação de insetos invasores .…”

Section: Introductionunclassified

Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

Nunes¹,

Galindo²,

Deus³

et al. 2009

RESUMO:A própolis é uma substância resinosa coletada pelas abelhas de diversas partes das plantas. Sua composição depende da época, vegetação e local de coleta. Apresenta diversas atividades biológicas como antimicrobiana, antioxidante, antitumoral, dentre outras. Foi realizado estudo da variabilidade sazonal, nos meses de fevereiro, junho e outubro de 2006, dos constituintes voláteis da própolis vermelha de Pernambuco através da extração por headspace dinâmico e identificação por cromatografia gasosa acoplada com espectrometria de massas (CG-EM). Foram identificados 34 constituintes voláteis, sendo monoterpenos e monoterpenóides, sesquiterpenos e sesquiterpenóides, fenilpropanóides, aldeídos, cetonas e η-alcanos. Os constituintes majoritários foram o trans-anetol, α-copaeno e o metil cis-isoeugenol. Também foi realizado o perfil fitoquímico por cromatografia em camada delgada (CCD), através da qual os constituintes fenólicos foram identificados como majoritários. Com o extrato bruto metanólico da própolis, realizou-se o ensaio de letalidade em Artemia salina, que demonstrou DL 50 de 18,9 µg/mL, sugerindo uma possível atividade antitumoral. Unitermos: Própolis vermelha, constituintes voláteis, Artemia salina.ABSTRACT: "Seasonal variability of red propolis constituents and brine shrimp bioassay". Propolis is a resinous hive product collected by honeybees from plant sources. The composition of the propolis depends on the season, the vegetation, and the area of collection. It presents a diverse biological activities such as antimicrobial, antioxidant and antitumoral. The seasonal variability of the red propolis constituents from Pernambuco State were accomplished in the months of February, June and October of 2006. The volatile red propolis constituients were captured by the dynamic headspace technique and analyzed by GC-MS. In the analysis of the samples, 34 constituents were identified as the mono and sesquiterpens, phenylpropanoids, aldehydes, cetons and n-alcanes, presenting as majority constituents the trans-anethol, α-copaene and methyl cis-isoeugenol. The phytochemistry profile was performed by thin layer chromatography (TLC). Through this technique the majority constituents were identified as phenols. The Brine shrimp bioassay was used to evaluate of the red propolis methanolic extract, which demonstrated a DL 50 of 18.9 µg/mL and an activity antitumoral was possibly suggested.

“…Furthermore, for herbal preparations, some tests include quantification A validated capillary gas chromatography method for guaco (Mikania glomerata S.) quality control and traceability: of the active compounds and uniformity of dosage, disintegration, dissolution among others (Ong, 2004). Concerning the quantification assay and considering the high complexity of this type of matrix, special attention has been given to chromatographic techniques, mainly gas chromatography (GC) and high-performance liquid chromatography (HPLC), due to their advantages such as good separation, efficiency and speed properties (WHO, 1998;Ribani et al, 2004;César et al, 2007;Sousa et al, 2007;Chang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A validated capillary gas chromatography method for guaco (Mikania glomerata S.) quality control and rastreability: from plant biomass to phytomedicines

Bueno¹,

Bastos²

2009

A validated capillary gas chromatography method for guaco (Mikania glomerata S.) quality control and rastreability: from plant biomass to phytomedicines ABSTRACT: This work describes a full validation of a capillary gas chromatography analytical methodology using internal standardization for the quantification of coumarin (1,2-benzopyrone) in guaco (Mikania glomerata Spreng -Asteraceae) products: syrup, plant and its extract, including the stability study of the phytomedicine. For the analysis, it was used an HP-5 capillary column (30 m x 0.32 mm x 0.25 µm), hydrogen at a flow rate of 1.8 mL/min and the increasing temperature gradient was: 100 °C to 250 °C, 15 ºC/min. The temperature of injector (split 1:20) and detector were kept at 250 °C and 270 °C, respectively. The retention times of the standards for the above conditions were 2.86 minutes for 1, 2, 3, 4-tetramethylbenzene, 4.45 min for piperonal (internal standards), and 5.36 minutes for coumarin. After extraction procedure, the recovery of coumarin determined for plant raw material was 101.6 %, while for syrup it was 100.8 %. Detection and quantification limits were 0.5 µg/mL and 1.5 µg/mL, respectively. Precision was determined for all samples and the results were lower than 2.5 %. The total amount of coumarin in plant raw material, its extract and syrup were 0.38 % w/w, 1.33 mg/mL and 0.143 mg/mL, respectively.