Analysis of 8-hydroxy-2′-deoxyguanosine in urine using high-performance liquid chromatography–electrospray tandem mass spectrometry

Electrochemical Determination of Oxidative Damaged DNA with High Sensitivity and Stability Using a Nanocarbon Film

“…5 Urinary 8-OHdG is known to be an important biomarker for risk assessment in certain fields including in relation to cancers, degenerative diseases, smoke and environmental health. 5 Urinary 8-OHdG has been analyzed using several methods, including high-performance liquid chromatography-electrochemical detection (HPLC-ECD), [7][8][9][10][11][12][13] GC-MS, 14 HPLC-tandem mass spectroscopy (LC-MS-MS) [15][16][17] and enzyme linked immunosorbent assay (ELISA) [18][19][20] for early diagnosis. The ELISA method enables us to detect a target with very high selectivity and sensitivity by amplifying the signal with an antibody and an enzyme.…”

Analysis of urinary 8-oxo-7,8-dihydro-purine-2’-deoxyribonucleosides by LC-MS/MS and improved ELISA

“…Broadly speaking the approaches to urinary 8-oxodG determination are either chromatographic, such as liquid chromatography with tandem mass spectrometry [6][7][8][9][10][11][12], and high performance liquid chromatography with electrochemical detection [13-Analysis of urinary 8-oxopurines 4 17]; or immunoassay, such as the commercial ELISA kit available from the Japanese Institute for the Control of Aging (JaICA) [18,19]. Immunoassay is clearly the most amenable approach, for most laboratories, not requiring expensive equipment, isotopically-labelled standards or specialist expertise.…”

Development of Point-of-Care Testing Sensors for Biomarker Detection

“…The traditional methods for 8-OHdG quantitative analysis are high-performance liquid chromatography (HPLC) with electrochemical detection (ECD), 91,92 gas chromatographymass spectrometry (GC-MS), 93 HPLC tandem mass spectrometry, 94,95 and Enzyme-linked Immunosorbent Assay (ELISA). This POCT device would provide results in minutes rather than days or weeks and eliminate the concerns involved with the transport and storage of biological samples.…”