Electrochemical Determination of Oxidative Damaged DNA with High Sensitivity and Stability Using a Nanocarbon Film

ONO‐2506 inhibits spike–wave discharges in a genetic animal model without affecting traditional convulsive tests via gliotransmission regulation

“…The concentration of kynurenic acid was determined by HPLC (ECD-300, Eicom) equipped with sputter deposited nanocarbon film electrode detection (NCE-HPLC) (Kato et al, 2011), set at +1600 mV (vs. an Ag/AgCl reference electrode). The analytical column (Triat C18 particle 2.0 mm, 150 ¥ 3.0 mm, YMC, Kyoto, Japan) was maintained at 25°C, and the flow rate of the mobile phase was set at 500 mL·min -1 .…”

“…Furthermore, the present study also determined the effects of ONO-2506 on the release of L-glutamate, D-serine, GABA and kynurenic acid, in the medial prefrontal cortex (mPFC) of freely moving rats and in primary cultured astrocytes by extreme liquid chromatography (xLC) (Yamamura et al, 2009b;Tanahashi et al, 2012) and HPLC equipped with nanocarbon film electrode detection (NCE-HPLC) (Kato et al, 2011).…”

Improved Direct Electrochemistry for Proteins Adsorbed on a UV/Ozone-Treated Carbon Nanofiber Electrode

“…They were used as base film electrodes, since they have been proved to provide good electron transfer that can successfully detect many redox species or biomolecules. 31,32 A low-pressure Hg lamp (EUV110GS-36 L, SEN Lights Corp., Japan) was used for the UV/ozone treatment. The CNFs were crushed to form a powder consisting of short rods.…”

Carbon-based Electrode Materials for DNA Electroanalysis

“…42,44,90,92,93 These characteristics allow the detection of biomolecules with slower electron transfer rates, such as NADPH and pyrimidine bases. 44,94 As a result, this film electrode can measure all DNA bases (including DNA base derivatives e.g., 5′-methylcytosine 46,69,70 and 8′-hydroxy 2′-deoxyguanosine 95 ) quantitatively. Moreover, due to their good electrochemical stability, these sp 3 -containing carbon electrodes are suitable for long-term analysis including as the electrode of an HPLC detector for detecting various biological compounds.…”

“…Moreover, due to their good electrochemical stability, these sp 3 -containing carbon electrodes are suitable for long-term analysis including as the electrode of an HPLC detector for detecting various biological compounds. 95,96 …”