Analysis of 5-hydroxy-7-methoxyflavones by normal-phase high-performance liquid chromatography

Herrera, Julio; Romero, Anthony; Crescente, Oscar; Acosta, Mercedes; Pékerar, Sara

doi:10.1016/0021-9673(96)00108-2

Cited by 15 publications

(5 citation statements)

References 18 publications

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“…In the NMR data of 13 C from compound 1 in the aliphatic zone (55.6 ppm), there appears a signal that belongs to a methoxy group. The signal in (43.1 ppm) is assigned to C-3 [26] and the signal in 103.5 ppm is assigned to C-10 of hydroxylated flavones, which are included in the structure of our compound and confirmed in the 1 H NMR spectrum. The spectral data of compound 2 show two signals in (55.6-55.7 ppm) belonging to two methoxy groups present in this structure, and these two groups are also observed in the 1 H NMR spectrum.…”

Section: Discussionsupporting

confidence: 61%

“…Natural flavonoids are often present in at least three phenolic hydroxyls [25]. The NMR 1 H data from compounds 1 and 2 show a substitution pattern "para" in the ring B [26]; substitutions in these flavones are found in the C-4 󸀠 position of the B ring; in the case of compound 1, the substituent corresponds to a hydroxyl group (OH), and for compound 2, the substituent corresponds to a methoxy group (OCH 3 ).…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical Study and Evaluation of the Cytotoxic Properties of Methanolic Extract from Baccharis obtusifolia

Romero-Benavides

Ortega-Torres

Villacis

et al. 2018

International Journal of Medicinal Chemistry

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Some species of the Baccharis genus have been shown to possess important biomedical properties, including cytotoxic activity. In this study, we examined the cytotoxic effect of methanol extract from Baccharis obtusifolia (Asteraceae) in cancer cell lines of prostate (PC-3), colon (RKO), astrocytoma (D-384), and breast (MCF-7). The methanolic extract displayed the largest substantial cytotoxic effect in lines of colon cancer (RKO) and cerebral astrocytoma (D-384). Chromatographic purification of the B. obtusifolia methanolic extract led to the isolation and identification of 5,4′-dihydroxy-7-methoxyflavone (1) and 5-hydroxy-7,4′-dimethoxyflavone (2) compounds of the flavonoid type.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Phytochemical Study and Evaluation of the Cytotoxic Properties of Methanolic Extract from Baccharis obtusifolia

Romero-Benavides

Ortega-Torres

Villacis

et al. 2018

International Journal of Medicinal Chemistry

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“…The 1 H NMR data of 4 are identical with published data (Herrera et al 1996). The EI-MS and 1 H NMR of 5 are identical with those of Seth et al (1982).…”

Section: Compounds Isolatedsupporting

confidence: 84%

Spasmolytic effects of Baccharis conferta and some of its constituents

Weimann

Göransson

Pongprayoon-Claeson

et al. 2002

Journal of Pharmacy and Pharmacology

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The Nahua of the Mexican state of Veracruz use Baccharis conferta in the treatment of a variety of gastrointestinal illnesses, especially diarrhoea associated with gastrointestinal cramps. The aerial parts of B. conferta were investigated phytochemically and pharmacologically using the guinea pig ileum assay as a model (histamine, KCI and electric stimulation). The crude ethanolic extract showed a dose-dependent antispasmodic effect that was particularly strong in flavonoid-rich fractions (e.g. IC50 value for fraction E.3.1 from the ethyl acetate fraction, in histamine-induced contraction, 10 microg mL(-1)). Several flavonoids (apigenin-4',7-dimethylether, naringenin-4',7-dimethylether, pectolinarigenin and cirsimaritin) were isolated, while others were identified in complex fractions by GC-MS. The flavonoids play an important role in the antispasmodic activity of this indigenous drug. Additionally, oleanolic acid and its methyl ester as well as erythrodiol were isolated. Oleanolic acid methyl ester shows weak antibacterial activity against M. luteusand E. coli (20 microg/spot in a TLC assay). The phytochemical as well as the pharmacological data provide some in-vitro evidence forthe use of B. conferta in thetreatment of gastrointestinal cramps.

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“…Baccharis trinervis leaves were extracted with chloroform, the extract was dried in vacuum, redissolved in 95% ethanol and macromolecules were precipitated by lead acetate (Herrera et al, 1996). Chromatographic analysis was performed on an amino-bonded column using hexane:-chloroform:acetonitrile gradient elution.…”

Section: High-performance Liquid Chromatography Of Avonoidsmentioning

confidence: 99%

Liquid chromatography of natural pigments

Cserháti

Forgács

Morais

et al. 2000

Biomed. Chromatogr.

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The newest results in the application of various liquid chromatographic techniques for the analysis of natural pigments in pure state and in complicated matrices are compiled. The methods employed for the separation and quantitative determination of the different pigment classes (flavonoids, coumarins, chlorophylls, etc.) are described and critically evaluated. The future trends are briefly discussed.

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Analysis of 5-hydroxy-7-methoxyflavones by normal-phase high-performance liquid chromatography

Cited by 15 publications

References 18 publications

Phytochemical Study and Evaluation of the Cytotoxic Properties of Methanolic Extract from Baccharis obtusifolia

Phytochemical Study and Evaluation of the Cytotoxic Properties of Methanolic Extract from Baccharis obtusifolia

Spasmolytic effects of Baccharis conferta and some of its constituents

Liquid chromatography of natural pigments

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