Liquid chromatography of natural pigments

Cserháti, Tibor; Forgács, Esther; Morais, Maria Helena; Mota, Teresa

doi:10.1002/1099-0801(200008)14:5<281::aid-bmc3>3.0.co;2-o

Cited by 23 publications

(22 citation statements)

References 39 publications

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“…The identification of reported compounds was based on their retention time, fragmentation pattern and high resolution mass data. All identified polyphenol compounds have been discovered in nature already [1, 15–26]. Out of the identified compounds, 46 were already reported to be found in Rhododendron species [1, 16–18].…”

Section: Resultsmentioning

confidence: 99%

Comparison of the polyphenolic profile and antibacterial activity of the leaves, fruits and flowers of Rhododendron ambiguum and Rhododendron cinnabarinum

et al. 2017

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Background Rhododendron species have been traditionally used in countries like China, Nepal, Russia and North America for treating human diseases. These species are known to be a good source of polyphenolic plant secondary plant metabolites. They are known to have beneficial health properties for humans and have been used to treat diseases like asthma, skin diseases. In this contribution we investigate the phenolic profile and antibacterial activity of extracts from several plant organs including for the first time from leaves of different development stages.MethodsIn this study, the polyphenolic profile of fruits, flowers and leaves of different ages of Rhododendron ambiguum and Rhododendron cinnabarinum were studied by using HPLC–MS and compounds identified based on high resolution masses and identity of tandem mass spectra, UV/VIS spectra and retention times if compared to standards.ResultsFifty-nine different polyphenols including isomers were identified in these species by their fragmentation pattern and high resolution data. Also, the antibacterial activity of these parts (leaves, fruits and flowers) against gram-positive bacteria was studied.ConclusionThe leaves and fruits contained more polyphenols than the flowers. With the exception of flowers, the fruits and leaves of both species were also determined to have a significant antibacterial effect against four gram-positive bacteria.Electronic supplementary materialThe online version of this article (doi:10.1186/s13104-017-2601-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Comparison of the polyphenolic profile and antibacterial activity of the leaves, fruits and flowers of Rhododendron ambiguum and Rhododendron cinnabarinum

et al. 2017

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“…In the food industry, pigments are used as additives, colour intensifiers, antioxidants, etc. Pigments represent extensive variety of colours, some of which are water-soluble (Cserháti 2006). Recently, colouring of food with pigments produced from natural sources is gaining worldwide interest and importance.…”

Section: Introductionmentioning

confidence: 99%

Prodigiosin and its potential applications

2015

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Since a decade, there has been a strong consumer demand for more natural products. This has augmented inclination towards substitution of synthetic colorants with natural pigments. Natural pigments not only have the capacity to increase the marketability of products, they also demonstrate valuable biological activities as antioxidants and anticancer agents. There is a long history of exploitation of natural products produced by bacteria as sources of pharmaceutically important, bioactive compounds. Among natural pigments, pigments from microbial sources are potentially suitable alternatives to synthetic pigments. The red pigment prodigiosin (PG) has unusual properties, which have long been documented. The red-pigmented prodiginines are bioactive secondary metabolites produced by both Gram-negative and Gram-positive bacteria. Prodigiosins are characterized by a common pyrrolyl pyrromethene skeleton, and the biological role of these pigments in the producer organisms remains unclear. Bacterial prodigiosins and their synthetic derivatives are effective proapoptotic agents against various cancer cell lines, with multiple cellular targets including multi-drug resistant cells with little or no toxicity towards normal cell lines. However, research into the biology of pigment production will stimulate interest in the bioengineering of strains to synthesize useful prodiginine derivatives. This review article highlights the characteristics and potential applications of prodigiosin pigment from Serratia as prodigiosins are real potential therapeutic drugs.

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“…The separations were done in the isocratic mode. The mobile phase and flow rate used for analysis of methyl orange, reactive blue 172 and congo red (10 mM ammonium acetate and acetonitrile (76:24), 0.8 mL/min; water and methanol (70:30), 1 mL/min; acetonitrile and water (60:40), 0.5 mL/min) . The retention time of reaction products from the redox dye were compared with the retention time of authentic standards under the same HPLC operating conditions.…”

Section: Methodsmentioning

confidence: 99%

The role of electrode material in capturing power generated in Pseudomonas catalysed fuel cells

Jayapriya

Ramamurthy

2013

Can J Chem Eng

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High efficiency electrodes (e.g. metal coated, carbon‐polymer composites and noble‐metal catalyst, etc.) used in conventional fuel cells are commercially available; however they are not being used in microbial fuel cells (MFCs). These electrodes are not biocompatible and more susceptible to catalyst poisoning when industrial effluents/aquatic sediments are used as a fuel. There is a need for unique electrode materials for MFCs that should be highly conductive material with enhanced active surface area, cheap, easy to process, biocompatible and scalable. In this study, the performance of mediator‐less microbial fuel cells (MFC) using Pseudomonas aeruginosa as a biocatalyst using different electrodes such as graphite blocks, carbon cloth, carbon fibre reinforced plate and graphite sheet was evaluated. Different metal salt doped graphite epoxy composites (MS‐GECE) and chemically modified carbon electrodes (CMCE) were also tested. Among the different combinations of the electrodes tested, Fe3+ graphite cathode produced significantly higher power density (1679.9 ± 98.04 µW/m2). These studies indicate the feasibility of the improvement in electrode design on the performance of MFC for wider applications.

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Liquid chromatography of natural pigments

Cited by 23 publications

References 39 publications

Comparison of the polyphenolic profile and antibacterial activity of the leaves, fruits and flowers of Rhododendron ambiguum and Rhododendron cinnabarinum

Comparison of the polyphenolic profile and antibacterial activity of the leaves, fruits and flowers of Rhododendron ambiguum and Rhododendron cinnabarinum

Prodigiosin and its potential applications

The role of electrode material in capturing power generated in Pseudomonas catalysed fuel cells

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