Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging

Imaging Strategies for Tissue Engineering Applications

“…The evaluation of bone ingrowth into various scaffolds has been demonstrated. [16][17][18] In one study, the mineralization stages at different distances from the scaffold interface were monitored using X-ray and provided insight into the complicated mineral deposition steps at the organic-mineral interface. 17 Also, micro-CT has been employed to evaluate the optimal culture conditions for bone mineralization of various scaffolds in vitro.…”

“…The functional capability of cells is commonly assessed by the ability of cells to uptake fluorodeoxyglucose ( 18 F-FDG), which is a glucose analog incorporating the positron-emitting radioactive isotope fluorine-18. Uptake of 18 F-FDG is correlated with tissue metabolism. The functional capability of an infarct region following delivery of progenitor cells has been assessed using FDG-PET imaging.…”

Treatment of osteochondral defects of the talus

“…151,258 MicroCT provides a fast and nondestructive technique to characterize and measure the 3-dimensional geometric and density properties of a bone specimen. 202 Gielkens et al compared the intraobserver reliability of μCT and histomorphometry. 151 Th ey concluded that both analyses are reliable but are used preferably in combination.…”

Advancements in three-dimensional titanium alloy mesh scaffolds fabricated by electron beam melting for biomedical devices: mechanical and biological aspects

“…The aspects that are of significance in the design and processing of optimal architecture for bone tissue engineering applications include: (a) high porosity-high strength combination with elastic modulus similar to bone, (b) interconnected porous structure with clinically relevant geometry that allows oxygen and exchange of nutrients, and facilitates efficient cell seeding and production of extracellular matrix (ECM), and (c) appropriate chemistry and topography for cell attachment, proliferation, and differentiation [33,34].…”