Analysis of 14C modern in coral Porites lobata using Liquid Scintillation Counting Method

Abstract

Analysis of 14 C modern in coral aims to determine the activity of 14 C modern of marine organism. Samples of living coral Porites lobata was taken from inner zone and middle inner zone, Spermonde Archipelago, Indonesia. This research used Liquid Scintillation Counting method. This method used three steps for sample preparation. The first step are physical and chemical washing. In this step, 9 % mass of sample has been removed from impurity. Second steps, CO2 Absorption. The sample entered in modificated absorption equipment and 14 CO2 captured with KOH solution. The last steps, 8 mL sample solution mixed with 12 mL scintillator and measured used LSC Hidex 300SL at counting times 5-240 minutes. The activity of 14 C modern in coral Porites lobata shown the differences activity between marine organism and land organism.

Optimization of Cocktail AquaLight LLT Volume for Estimation of 14C Activity in Aqueous Sample using LSC Hidex 300 SL

“…14 C analysis by mixing 8 mL sample and 12 mL aquaLight LLT in vial 20 mL [23]. Analysis of 14 C sample from coral Porites Lobata with the ratio between sample and scintillator is 8:12 [24]. Analysis 14 C used 8-12 mL of Carbo-Sorp E and mixture with Permaflour E+ scintillation cocktail and then analysis with LSC [8].…”