Analysis by High-Performance Liquid Chromatography Diode Array Detection Mass Spectrometry of Phenolic Compounds in Fruit of Eucalyptus globulus Cultivated in Algeria

Abstract

A method based on high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) following fractionation by chromatography on a Sephadex LH-20 column has been developed to determine the phenolic composition of fruit of Eucalyptus globulus growing in Algeria. The presence of 18 gallotannins, 26 ellagitannins, and 2 flavonols was established. Tentative identification is provided for these compounds on the basis of UV-visible spectra and mass spectrometry data. Most compounds described in this study have not previously detected in fruit of E. globulus. Moreover, this is the first report of methyl digalloyl diglucose, 3,3'-O-dimethylellagic acid 4-O-β-glucopyranoside, ellagic acid hexose, methyl ellagic acid pentose, methyltetragalloylglucose, and valoneic acid isomers (sanguisorbic, flavogallic acid dilactone) in the genus Eucalyptus. Quantitatively, ellagic acid and its derivatives, including ellagitannins, are largely predominant.

Antioxidant capacity and phenolics of Pouteria macrophylla, an under-utilized fruit from Brazilian Amazon

“…Analysis of chromatographic proWle did not showed signals at 520 nm, suggesting the absence of anthocyanins in the extract [23]. It was also ruled out the presence of ellagic acid by the absence of maximum absorbance in 250 and 365 nm (24). The chromatogram (Fig.…”

“…1) showed two major peaks (A and B) close to the retention times of 10 and 20 min, respectively, which can be assumed as belonging to the group of phenolic acids and their derivatives. The peaks A and B showed maximal absorbance in range from 265 to 280 nm which matches with that of gallic acid ( max » 275 nm) [24], as well as the UV spectra of peak A and gallic acid standard were superimposed, conWrming its identiWcation. HPLC-DAD showed that the peak B is a gallic acid derivative.…”

Investigation of the antioxidant capacity and phenolic constituents of U.S. pecans

“…Retention time (tR) from RP-HPLC analysis. b Tentative identifications were achieved using RT mapping and fragmentation-pattern comparison to those of commercial standards as well as compounds previously reported in plant material byBoulekbache-Makhlouf et al (2010) and pecans by de la Rosa et al…”

Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus

“…Eucalyptus is one of the world's important and most widely planted genera [2] . It is a tall, evergreen tree, native to Australia and Tasmania, successfully introduced worldwide, now extensively planted in many other countries [3] .It was introduced in Algeria in 1854 by Ramel [4] . Eucalyptus species are well known as medicinal plants because of their biological and pharmacological properties.…”