Analysis by coagglutination of the distribution of a 24,000-dalton surface protein in Yersinia isolates

Toyos, Juan R. de los; Diaz, Roger; E, Urra; Moriyón, Ignacio

doi:10.1128/jcm.23.4.804-805.1986

Cited by 8 publications

(1 citation statement)

References 4 publications

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“…Presence or absence of pYV was confirmed by detection of protein YadA by an autoagglutination test (38). The mucoid Yersinia factor (Myf) was detected by coagglutination (41).…”

Section: Methodsmentioning

confidence: 99%

Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides

1996

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Sensitivities to polycationic peptides and EDTA were compared in Yersinia enterocolitica pathogenic and environmental biogroups. As shown by changes in permeability to the fluorescent hydrophobic probe Nphenylnaphthylamine (NPN), the outer membranes (OMs) of pathogenic and environmental strains grown at 26؇C in standard broth were more resistant to poly-L-lysine, poly-L-ornithine, melittin, cecropin P1, polymyxin B, and EDTA than Escherichia coli OMs. At 37؇C, OMs of pathogenic biogroups were resistant to EDTA and polycations and OMs of environmental strains were resistant to EDTA whereas E. coli OMs were sensitive to both EDTA and polycations. Similar results were found when testing deoxycholate sensitivity after polycation exposure or when isogenic pairs with or without virulence plasmid pYV were compared. With bacteria grown without Ca ؉؉ available, OM permeability to NPN was drastically increased in pathogenic but not in environmental strains or E. coli. Under these conditions, OMs of pYV ؉ and pYV ؊ cells showed small differences in NPN permeability but differences in polycation sensitivity could not be detected by fluorimetry. O:1,6 (environmental type) lipopolysaccharide (LPS), but not O:3 or O:8 LPS, was markedly rough at 37؇C, and this could explain the differences in polycation sensitivity. LPSs from serotypes O:3 and O:8 grown at 37؇C were more permeable to NPN than O:1,6 LPS, and O:8 LPS was resistant to polycation-induced permeabilization. These data suggest that LPSs relate to some but not all the OM differences described. It is hypothesized that the different OM properties of environmental and pathogenic biogroups reflect the adaptation of the latter biogroups to pathogenicity.

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“…Presence or absence of pYV was confirmed by detection of protein YadA by an autoagglutination test (38). The mucoid Yersinia factor (Myf) was detected by coagglutination (41).…”

Section: Methodsmentioning

confidence: 99%