Analysis and quantification of vitamin D metabolites in serum by ultra‐performance liquid chromatography coupled to tandem mass spectrometry and high‐resolution mass spectrometry – a method comparison and validation

Bruce, Stephen J.; Rochat, Bertrand; Béguin, Alexandre; Pesse, Benoît; Guessous, Idris; Boulat, Olivier; Henry, Hugues

doi:10.1002/rcm.6439

Cited by 74 publications

(48 citation statements)

References 38 publications

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“…Nevertheless, Bland-Altman analysis did not show any concentration-dependent or systematic differences between HRMS and DiaSorin (Figure 3d). These results are mostly in agreement with findings from other studies using Orbitraps for HRMS, where generally strong correlations between Orbitrap and QqQ [15], and Orbitrap and IDS-iSYS 25-hydroxyvitamin D immunoassay [18] were shown.…”

Section: Clinical Samples From Patient Studysupporting

confidence: 92%

“…Although narrowing the window size clearly reduced the abundances of isobars in the TOF traces, the problem of isobaric cross-talk persisted, with resulting unsatisfactory signal-to-noise ratios and insufficient precision. Clearly, the higher resolving power obtained from an Orbitrap would be beneficial in this situation [15,18]. There was little influence on peak areas in the CID-MS/MS traces when the extraction size was reduced, however, as isobaric noise generally was very low in the CID spectra (Figure 1d).…”

Section: Hrms: Tof-ms Versus Tof-ms/msmentioning

confidence: 99%

“…Mass analyzers of medium/high mass resolving power such as time-of-flight (TOF), Orbitrap, and Fourier transform ion cyclotron resonance (FTICR) have not yet routinely entered clinical practice, even though their potential has been clearly recognized [13,14]. Furthermore, a few recent studies have successfully demonstrated the potential of HRMS in the quantification of vitamin D compounds [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Triple Quadrupole Versus High Resolution Quadrupole-Time-of-Flight Mass Spectrometry for Quantitative LC-MS/MS Analysis of 25-Hydroxyvitamin D in Human Serum

Geib

Sleno

Hall

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. We describe a systematic comparison of high and low resolution LC-MS/ MS assays for quantification of 25-hydroxyvitamin D 3 in human serum. Identical sample preparation, chromatography separations, electrospray ionization sources, precursor ion selection, and ion activation were used; the two assays differed only in the implemented final mass analyzer stage; viz. high resolution quadrupolequadrupole-time-of-flight (QqTOF) versus low resolution triple quadrupole instruments. The results were assessed against measured concentration levels from a routine clinical chemiluminescence immunoassay. Isobaric interferences prevented the simple use of TOF-MS spectra for extraction of accurate masses and necessitated the application of collision-induced dissociation on the QqTOF platform. The two mass spectrometry assays provided very similar analytical figures of merit, reflecting the lack of relevant isobaric interferences in the MS/MS domain, and were successfully applied to determine the levels of 25-hydroxyvitamin D for patients with chronic liver disease.

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Section: Clinical Samples From Patient Studysupporting

confidence: 92%

Section: Hrms: Tof-ms Versus Tof-ms/msmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Triple Quadrupole Versus High Resolution Quadrupole-Time-of-Flight Mass Spectrometry for Quantitative LC-MS/MS Analysis of 25-Hydroxyvitamin D in Human Serum

Geib

Sleno

Hall

et al. 2016

J. Am. Soc. Mass Spectrom.

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“…A fast, accurate, and reliable method to quantify the vitamin D metabolites-25-hydroxyvitamin D 2 , 25-hydroxyvitamin D 3 , and 3-epi-25hydroxyvitamin D 3 -in human serum samples was developed and validated for this project (21). An ultrahigh-pressure liquid chromatography-tandem mass spectrometry system was developed.…”

Section: (Oh)dmentioning

confidence: 99%

Serum 25-Hydroxyvitamin D Level and Kidney Function Decline in a Swiss General Adult Population

Guessous

McClellan

Kleinbaum

et al. 2015

Clinical Journal of the American Society of Nephrology

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Background and objectives Molecular evidence suggests that levels of vitamin D are associated with kidney function loss. Still, population-based studies are limited and few have considered the potential confounding effect of baseline kidney function. This study evaluated the association of serum 25-hydroxyvitamin D with change in eGFR, rapid eGFR decline, and incidence of CKD and albuminuria.Design, setting, participants, & measurements Baseline (2003Baseline ( -2006 and 5.5-year follow-up data from a Swiss adult general population were used to evaluate the association of serum 25-hydroxyvitamin D with change in eGFR, rapid eGFR decline (annual loss .3 ml/min per 1.73 m 2 ), and incidence of CKD and albuminuria. Serum 25-hydroxyvitamin D was measured at baseline using liquid chromatography-tandem mass spectrometry. eGFR and albuminuria were collected at baseline and follow-up. Multivariate linear and logistic regression models were used considering potential confounding factors.Results Among the 4280 people included in the analysis, the mean6SD annual eGFR change was 20.5761.78 ml/min per 1.73 m 2 , and 287 (6.7%) participants presented rapid eGFR decline. Before adjustment for baseline eGFR, baseline 25-hydroxyvitamin D level was associated with both mean annual eGFR change and risk of rapid eGFR decline, independently of baseline albuminuria. Once adjusted for baseline eGFR, associations were no longer significant. For every 10 ng/ml higher baseline 25-hydroxyvitamin D, the adjusted mean annual eGFR change was 20.005 ml/min per 1.73 m 2 (95% confidence interval, 20.063 to 0.053; P=0.87) and the risk of rapid eGFR decline was null (odds ratio, 0.93; 95% confidence interval, 0.79 to 1.08; P=0.33). Baseline 25-hydroxyvitamin D level was not associated with incidence of CKD or albuminuria.Conclusions The association of 25-hydroxyvitamin D with eGFR decline is confounded by baseline eGFR. Sufficient 25-hydroxyvitamin D levels do not seem to protect from eGFR decline independently from baseline eGFR.

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“…Previously, the quantitative aspects of low resolution MS (LR-MS) operated in SRM mode has been compared with several different modes in HR-MS [21][22][23][24][25][26][27][28]. Comparisons of selectivity were less often subject of investigation.…”

Section: Introductionmentioning

confidence: 99%

The Assessment of Selectivity in Different Quadrupole-Orbitrap Mass Spectrometry Acquisition Modes

Berendsen

Wegh

Meijer

et al. 2014

J. Am. Soc. Mass Spectrom.

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Abstract. Selectivity of the confirmation of identity in liquid chromatography (tandem) mass spectrometry using Q-Orbitrap instrumentation was assessed using different acquisition modes based on a representative experimental data set constructed from 108 samples, including six different matrix extracts and containing over 100 analytes each. Single stage full scan, all ion fragmentation, and product ion scanning were applied. By generating reconstructed ion chromatograms using unit mass window in targeted MS 2 , selected reaction monitoring (SRM), regularly applied using triplequadrupole instruments, was mimicked. This facilitated the comparison of single stage full scan, all ion fragmentation, (mimicked) SRM, and product ion scanning applying a mass window down to 1 ppm. Single factor Analysis of Variance was carried out on the variance (s 2 ) of the mass error to determine which factors and interactions are significant parameters with respect to selectivity. We conclude that selectivity is related to the target compound (mainly the mass defect), the matrix, sample clean-up, concentration, and mass resolution. Selectivity of the different instrumental configurations was quantified by counting the number of interfering peaks observed in the chromatograms. We conclude that precursor ion selection significantly contributes to selectivity: monitoring of a single product ion at high mass accuracy with a 1 Da precursor ion window proved to be equally selective or better to monitoring two transition products in mimicked SRM. In contrast, monitoring a single fragment in all ion fragmentation mode results in significantly lower selectivity versus mimicked SRM. After a thorough interlaboratory evaluation study, the results of this study can be used for a critical reassessment of the current identification points system and contribute to the next generation of evidence-based and robust performance criteria in residue analysis and sports doping.

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Analysis and quantification of vitamin D metabolites in serum by ultra‐performance liquid chromatography coupled to tandem mass spectrometry and high‐resolution mass spectrometry – a method comparison and validation

Cited by 74 publications

References 38 publications

Triple Quadrupole Versus High Resolution Quadrupole-Time-of-Flight Mass Spectrometry for Quantitative LC-MS/MS Analysis of 25-Hydroxyvitamin D in Human Serum

Triple Quadrupole Versus High Resolution Quadrupole-Time-of-Flight Mass Spectrometry for Quantitative LC-MS/MS Analysis of 25-Hydroxyvitamin D in Human Serum

Serum 25-Hydroxyvitamin D Level and Kidney Function Decline in a Swiss General Adult Population

The Assessment of Selectivity in Different Quadrupole-Orbitrap Mass Spectrometry Acquisition Modes

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