Analysis and occurrence of residues of the hormones estriol, 17α- ethinylestradiol and 17β-estradiol in urban water supply by HPLC-DAD

Torres, Nádia Hortense; Ferreira, Luiz Fernando Romanholo; Andrade, Graziela Cristina Rossi de Moura; Tornisielo, Valdemar Luiz

doi:10.9790/3021-0205984989

Cited by 10 publications

(7 citation statements)

References 25 publications

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“…The PolarScreen assay was used to test the ligand binding affinity of wt-ER α LBD and its mutants towards different classes of estrogen disrupting compounds. The tested chemical compounds (shown in Figure 4 ) were: the natural ligand 17β-estradiol; its close analog 17α-ethynilestradiol (chosen since it is an emerging pollutant [42] ); tamoxifen, a commercial drug antagonist often detected in surface waters [43] ; and three other compounds with known estrogen disrupting activity and significant presence in the environment, bisphenol-A, 4-nonylphenol and 4-tert-octylphenol [44] . The latter three have micromolar binding affinity whereas the first three have nanomolar affinity for wild type ER [45] .…”

Section: Resultsmentioning

confidence: 99%

Rational Modification of Estrogen Receptor by Combination of Computational and Experimental Analysis

Ferrero

Pedotti²,

Chiadò³

et al. 2014

PLoS ONE

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In this manuscript, we modulate the binding properties of estrogen receptor protein by rationally modifying the amino acid composition of its ligand binding domain. By combining sequence alignment and structural analysis of known estrogen receptor-ligand complexes with computational analysis, we were able to predict estrogen receptor mutants with altered binding properties. These predictions were experimentally confirmed by producing single point variants with up to an order of magnitude increased binding affinity towards some estrogen disrupting chemicals and reaching an half maximal inhibitory concentration (IC50) value of 2 nM for the 17α-ethinylestradiol ligand. Due to increased affinity and stability, utilizing such mutated estrogen receptor instead of the wild type as bio-recognition element would be beneficial in an assay or biosensor.

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Section: Resultsmentioning

confidence: 99%

Rational Modification of Estrogen Receptor by Combination of Computational and Experimental Analysis

Ferrero

Pedotti²,

Chiadò³

et al. 2014

PLoS ONE

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“…The average annual rainfall varies from 1300 to 1800 mm. Monthly averages indicate that the least rainy months are July and August and the highest rainfall occurring in December and January (Torres et al 2012).…”

Section: Characterization Of the Study Areamentioning

confidence: 99%

“…These first reports resulted in the detection of certain drugs in wastewater from STPs in the USA. Several studies have also revealed the presence of PhCs in bodies of water in many other countries (Sirés and Brillas 2012;Torres et al 2012). Hormones have been detected in wastewater, surface water, and groundwater in various regions around the world in concentrations from nanograms to micrograms per liter (Farré et al 2008;López-Serna et al 2010;Alda and Barceló 2001).…”

Section: Introductionmentioning

confidence: 95%

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Detection of hormones in surface and drinking water in Brazil by LC-ESI-MS/MS and ecotoxicological assessment with Daphnia magna

Torres

Aguiar

Ferreira

et al. 2015

Environ Monit Assess

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The growing use of pharmaceutical drug is mainly due to several diseases in human and in animal husbandry. As these drugs are discharged into waterways via wastewater, they cause a major impact on the environment. Many of these drugs are hormones; in which even at low concentrations can alter metabolic and physiological functions in many organisms. Hormones were found in surface water, groundwater, soil, and sediment at concentrations from nanograms to milligrams per liter of volume--quantities known to cause changes in the endocrine system of aquatic organisms. This study aimed to develop a methodology for hormone detection (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) on surface and treated water samples. Sample toxicity was assessed by ecotoxicology tests using Daphnia magna. A liquid chromatograph coupled to a mass spectrometer with an electrospray ionization source (LC-ESI-MS/MS) was used for the analysis. The results showed that samples were contaminated by the hormones estriol, estrone, progesterone, 17β-estradiol, and 17α-ethinylestradiol during the sampling period, and the highest concentrations measured were 90, 28, 26, 137, and 194 ng · L(-1), respectively. This indicates the inflow of sewage containing these hormones at some points in the Piracicaba River in the State of Sao Paulo-Brazil. Results indicated little toxicity of the hormone estriol in D. magna, indicating that chronic studies with this microcrustacean are necessary.

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“…A presença de hormônios femininos em águas superfi ciais, águas subterrâneas, águas resíduárias e até mesmo em águas para consumo humano têm sido relatada em diversos países como Inglaterra, Brasil, Alemanha, Canadá, Itália, Holanda, Estados Unidos e Suécia (DESBROW et al, 1998;LARSSON et al, 1999;TERNES et al, 1999;JONHSON, BELFROID e CORCIA, 2000;KOLPIN et al, 2002;TORRES et al, 2012).…”

Section: Ocorrência De Desreguladores Endócrinos No Ambienteunclassified

Desreguladores Endócrinos No Ambiente E Seus Efeitos Na Biota E Saúde Humana

Américo

Messias

Torres

et al. 2012

PES

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O presente artigo teve como objetivo efetuar revisão de literatura sobre os desreguladores endócrinos. Os tópicos abordados incluíram a caracterização e a classificação dos desreguladores endócrinos, sua ocorrência no ambiente, possíveis efeitos na biota e na saúde humana. De acordo com a pesquisa bibliográfica, ainda existem poucos estudos no Brasil relatando a ocorrência de desreguladores endócrinos no ambiente e seus possíveis efeitos nos seres vivos. Tais trabalhos se concentram em países desenvolvidos, localizados na maioria das vezes em regiões temperadas. Essas pesquisas indicam que mesmo em baixas concentrações, os desreguladores endócrinos afetam toda a biota e causam prejuízos à saúde humana, interferindo principalmente no sistema reprodutor dos organismos. O estudo dos desreguladores endócrinos torna-se importante para definição de medidas que minimizem sua liberação no ambiente e para o desenvolvimento de metodologias que reduzam ou removam essas substâncias das matrizes ambientais.

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Analysis and occurrence of residues of the hormones estriol, 17α- ethinylestradiol and 17β-estradiol in urban water supply by HPLC-DAD

Cited by 10 publications

References 25 publications

Rational Modification of Estrogen Receptor by Combination of Computational and Experimental Analysis

Rational Modification of Estrogen Receptor by Combination of Computational and Experimental Analysis

Detection of hormones in surface and drinking water in Brazil by LC-ESI-MS/MS and ecotoxicological assessment with Daphnia magna

Desreguladores Endócrinos No Ambiente E Seus Efeitos Na Biota E Saúde Humana

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