Analyses of the probiotic property and stress resistance-related genes of Lactococcus lactis subsp. lactis NCDO 2118 through comparative genomics and in vitro assays

Abstract

Lactococcus lactis subsp. lactis NCDO 2118 was recently reported to alleviate colitis symptoms via its anti-inflammatory and immunomodulatory activities, which are exerted by exported proteins that are not produced by L. lactis subsp. lactis IL1403. Here, we used in vitro and in silico approaches to characterize the genomic structure, the safety aspects, and the immunomodulatory activity of this strain. Through comparative genomics, we identified genomic islands, phage regions, bile salt and acid stress resistance genes, bacteriocins, adhesion-related and antibiotic resistance genes, and genes encoding proteins that are putatively secreted, expressed in vitro and absent from IL1403. The high degree of similarity between all Lactococcus suggests that the Symbiotic Islands commonly shared by both NCDO 2118 and KF147 may be responsible for their close relationship and their adaptation to plants. The predicted bacteriocins may play an important role against the invasion of competing strains. The genes related to the acid and bile salt stresses may play important roles in gastrointestinal tract survival, whereas the adhesion proteins are important for persistence in the gut, culminating in the competitive exclusion of other bacteria. Finally, the five secreted and expressed proteins may be important targets for studies of new anti-inflammatory and immunomodulatory proteins. Altogether, the analyses performed here highlight the potential use of this strain as a target for the future development of probiotic foods.

A Controlled Fermented Samjunghwan Herbal Formula Ameliorates Non-alcoholic Hepatosteatosis in HepG2 Cells and OLETF Rats

“…Both L. brevis and L. lactis , the two most abundant bacterial species found in our FSJH preparation, are lactic acid bacteria, which are known to include many species with probiotic properties ( Ljungh and Wadstrom, 2006 ). Additionally, several lines of evidence indicate the strain-specific probiotic activities of L. lactis ( Yadav et al, 2009 ; Han et al, 2015 ; Lee et al, 2015 ; Armas et al, 2017 ; Oliveira et al, 2017 ) and L. brevis ( Rönkä et al, 2003 ; Linsalata et al, 2004 ; Fukao et al, 2013 ; Waki et al, 2014 ). Moreover, for the past few years, a large number of studies have revealed the probiotic health effects of L. plantarum ( Seddik et al, 2017 ).…”

Comparative proteomic analysis of four biotechnological strains Lactococcus lactis through label‐free quantitative proteomics

“…The adhesion cellular process enhances the probiotic effect this interaction between the microbe-host stimulates the immunomodulation process, as well as protects the intestinal barrier against pathogens (Bron et al, 2011). We have identified proteins as basic membrane protein A, Chitinase, Exodeoxyribonuclease, Pyruvate carboxylase fibronectin-binding protein, which contribute to adhesion cellular of L. lactis (Radziwill-Bienkowska et al, 2016;Oliveira et al, 2017). In addition, the L. lactis core proteome is composed by components important of the cell wall this bacterium related to peptidoglycan, capsular polysaccharide (CPS), lipoproteins and teichoic acids, which contain specific structures that are recognized by Toll-like receptor present in the host intestinal mucosa (Bron et al, 2011).…”

Characterization of a potential probiotic bacterium Lactococcus raffinolactis WiKim0068 isolated from fermented vegetable using genomic and in vitro analyses

“…Strain WiKim0068 had only vanW gene among the vancomycin resistance gene cluster, and the function of this gene is still unknown. The safety of L. lactis strains has not yet been assured through the comparison of antibiotic susceptibility profiles and the presence of the genes putatively encoding antibiotic resistance-related proteins [29,30]. The analysis of L. raffinolactis WiKim0068 based on ResFinder 3.0 did not detect antimicrobial resistance genes against aminoglycoside, beta-lactam, colistin, fluoroquinolone, fosfomycin, fusidic acid, glycopeptide, macrolide-lincosamidestreptogramin B, nitroimidazole, oxazolidinone, phenicol, rifampicin, sulphonamide, tetracycline, or trimethoprim.…”