Comparative proteomic analysis of four biotechnological strains <i>Lactococcus lactis</i> through label‐free quantitative proteomics

Silva, Wanderson M.; Sousa, Cassiana Severiano de; Oliveira, Lívia Costa de; Soares, Siomar C.; Souza, Gustavo H. M. F.; Tavares, Guilherme Campos; Resende, Cristiana P.; Folador, Edson Luiz; Pereira, Felipe L.; Figueiredo, Henrique César Pereira; Azevedo, Vasco

doi:10.1111/1751-7915.13305

Cited by 13 publications

(11 citation statements)

References 64 publications

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“…These analyses may even contribute to the development of strategies to increase the biotechnological potential of this species. In a recent study, the L. lactis core proteome was characterized, using a two-dimensional re-versed phase nano ultra-performance LC-MS approach, in four L. lactis strains including MG1363 (42). Overall, 1108 proteins were detected in at least two of the three biological replicates for the four strains.…”

Section: Discussionmentioning

confidence: 99%

Investigating Lactococcus lactis MG1363 Response to Phage p2 Infection at the Proteome Level

Lemay

Otto

Maaß

et al. 2019

Molecular & Cellular Proteomics

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The cellular response of Lactococcus lactis MG1363 to infection by the virulent phage p2 was characterized through labelfree quantitative proteomics. Combined with a targeted approach, we identified the highest proteome coverage for phage p2, a model for the most predominant group of lactococcal phages in the dairy industry worldwide. Four bacterial gene candidates possibly involved in phage infection were disrupted through specific knockouts to investigate their importance. Deletion of gene llmg_0219 (unknown function) resulted in a resistance to phage p2. Graphical Abstract Highlights• The proteomes of L. lactis MG1363 and phage p2 at different stages of infection were characterized.• 16% (226/1412) of the bacterial proteins detected were unique to infected cultures.• A targeted approach using synthetic peptides improved the coverage of phage p2 proteome.• By means of proteogenomics, we uncovered a conserved phage protein coded by a previously unannotated gene.• Deletion of the bacterial gene llmg_0219 (unknown function) impedes phage p2 infection.

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Section: Discussionmentioning

confidence: 99%

Investigating Lactococcus lactis MG1363 Response to Phage p2 Infection at the Proteome Level

Lemay

Otto

Maaß

et al. 2019

Molecular & Cellular Proteomics

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“…Protein identification was performed according to a reported method. 31 P ≤ .05 and absolute value of ratio ≥ 1.2 were set as thresholds to determine significant differentially expressed proteins (DEPs). Pathway analyses were used to identify significant pathways of DEPs according to Kyoto Encyclopedia of Genes and Genomes (KEGG) database (http://www.genome.ad.jp/kegg/).…”

Section: Proteomics Analysesmentioning

confidence: 99%

Targeted overexpression of PPARγ in skeletal muscle by random insertion and CRISPR/Cas9 transgenic pig cloning enhances oxidative fiber formation and intramuscular fat deposition

Zhou

Yang

et al. 2021

FASEB j.

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Peroxisome proliferator-activated receptor gamma (PPARγ) is a master regulator of adipogenesis and lipogenesis. To understand its roles in fiber formation and fat deposition in skeletal muscle, we successfully generated muscle-specific overexpression of PPARγ in two pig models by random insertion and CRISPR/Cas9 transgenic cloning procedures. The content of intramuscular fat was significantly increased in PPARγ pigs while had no changes on lean meat ratio. PPARγ could promote adipocyte differentiation by activating adipocyte differentiating regulators such as FABP4 and CCAAT/enhancer-binding protein (C/EBP), along with enhanced expression of LPL, FABP4, and PLIN1 to proceed fat deposition. Proteomics analyses demonstrated that oxidative metabolism of fatty acids and respiratory chain were activated in PPARγ pigs, thus, gathered more Ca 2+ in PPARγ pigs. Raising of Ca 2+ could result in increased phosphorylation of CAMKII and p38 MAPK in PPARγ pigs, which can stimulate MEF2 and PGC1α to affect fiber type and oxidative capacity. These results support that skeletal muscle-specific overexpression of PPARγ can promote oxidative fiber formation and intramuscular fat deposition in pigs.

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“…lactis. These findings provide important knowledge about the core proteome of these bacteria, very important from the biotechnological point of view to improve the understanding of cell functions and thus optimize their use in bioprocesses, mainly as starter cultures in fermentation stages [87].…”

Section: Study Of the Behavior Of Microorganisms During The Fermentation And Maturation Stagesmentioning

confidence: 91%

Foodomic-Based Approach for the Control and Quality Improvement of Dairy Products

et al. 2021

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The food quality assurance before selling is a needed requirement intended for protecting consumer interests. In the same way, it is also indispensable to promote continuous improvement of sensory and nutritional properties. In this regard, food research has recently contributed with studies focused on the use of ‘foodomics’. This review focuses on the use of this technology, represented by transcriptomics, proteomics, and metabolomics, for the control and quality improvement of dairy products. The complex matrix of these foods requires sophisticated technology able to extract large amounts of information with which to influence their aptitude for consumption. Thus, throughout the article, different applications of the aforementioned technologies are described and discussed in essential matters related to food quality, such as the detection of fraud and/or adulterations, microbiological safety, and the assessment and improvement of transformation industrial processes (e.g., fermentation and ripening). The magnitude of the reported results may open the door to an in-depth transformation of the most conventional analytical processes, with the introduction of new techniques that allow a greater understanding of the biochemical phenomena occurred in this type of food.

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Comparative proteomic analysis of four biotechnological strains Lactococcus lactis through label‐free quantitative proteomics

Cited by 13 publications

References 64 publications

Investigating Lactococcus lactis MG1363 Response to Phage p2 Infection at the Proteome Level

Investigating Lactococcus lactis MG1363 Response to Phage p2 Infection at the Proteome Level

Targeted overexpression of PPARγ in skeletal muscle by random insertion and CRISPR/Cas9 transgenic pig cloning enhances oxidative fiber formation and intramuscular fat deposition

Foodomic-Based Approach for the Control and Quality Improvement of Dairy Products

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