Analyses of the modulatory effects of antibacterial silver doped calcium phosphate-based ceramic nano-powder on proliferation, survival, and angiogenic capacity of different mammalian cells in vitro

Abstract

In this study, the antibacterial, cytotoxic, and angiogenic activities of silver doped calcium phosphate-based inorganic powder (ABT or PAG) were systematically investigated. ABT powders containing varying silver content were fabricated using a wet chemical manufacturing method. Antibacterial efficiencies of the ABT powders were investigated using a standard test with indicator bacteria and yeast. The cytotoxic effects of ABT on three different fibroblast cells and human umbilical vein endothelial cells (HUVECs) were assessed using MTT assay. ABT powder exhibits concentration-related cytotoxicity characteristics. Apoptotic activity, attachment capability, and wound healing effects were examined on fibroblasts. The angiogenic activity of ABT was investigated by tube formation assay in HUVECs; 10 μg ml(-1) and 100 μg ml(-1) concentrations of the highest metal ion content of ABT did not disrupt the tube formation of HUVECs. All these tests showed that ABT does not compromise the survival of the cells and might impose regeneration ability to various cell types. These results indicate that silver doped calcium phosphate-based inorganic powder with an optimal silver content has good potential for developing new biomaterials for implant applications.

Atomic Layer Deposition of a Silver Nanolayer on Advanced Titanium Orthopedic Implants Inhibits Bacterial Colonization and Supports Vascularized de Novo Bone Ingrowth

“…However, in vitro responses can be cell type specific as high concentrations of labile Ag‐containing surface coatings are known to be toxic to human fetal osteoblasts . We also found that both attachment and growth of HDMEC is well tolerated on Ag‐coated surfaces, similar to that described for human umbilical vein endothelial cells grown on silver‐doped ceramic nanopowder . In a mouse model where higher concentrations of Ag‐nanoparticles are released into the microcirculation, significant toxicity on endothelium has also been reported .…”

Silver ion‐doped calcium phosphate‐based bone‐graft substitute eliminates chronic osteomyelitis: An experimental study in animals

“…Both pure and silver ion doped calcium phosphate‐based micro and macro porous ceramic beads were fabricated using wet chemically synthesized calcium phosphate powders. Synthesis procedure of the silver ion doped calcium phosphates and the results of the silver release and toxicity studies has been reported in the previous studies 19,20,27,28 . In general, silver nitrate was dispersed and mixed with calcium hydroxide in pure water.…”

“…Our group has previously reported the development of a new silver ion doped nano‐size calcium phosphate‐based ceramic powder 19,20,27 . It was demonstrated that the coating prevented infection and did not disturb angiogenesis which plays a key role in osseous tissue growth, and at used concentration it was not cytotoxic 28 . After successful use of this material as a coating to prevent implant‐associated infections, it was thought that it would also be effective in the treatment of bone infection as a bone graft substitute.…”

Chitosan/Clay Bionanocomposites: Structural, Antibacterial, Thermal and Swelling Properties

“…This may be caused by the fact that in the agar diffusion test of chitosan, the agar did not diffuse through the medium, so that only the organism in direct contact with the active site of chitosan was inhibited. 1,13,15,18 Another reason can be that S. aureus and E. coli bacteria that were used may have gained resistance to chitosan. Bacteria can increase their resilience over time to survive.…”