Here, we present a brief introduction to sixteen full-text research articles of the Special Issue "Progress in Slovak Botany: a successful path to modern research on flora and vegetation" which are more-less a reflection of the current state of the study of botany in Slovakia. The papers bring results of classification and syntaxonomical revision of shrubs and forest vegetation, followed by research into vegetation diversity, ecology, and taxonomy. Special attention is paid to molecular methods in the research of plant taxa; one of the contributions evaluates the use of these methods in the study of population genetics of forest trees in Slovakia in the past 40 years. The other three papers use the knowledge of flow cytometry for a taxonomic study of selected representatives of the genera Tanacetum, Cotoneaster, Solanum and Nicotiana. A special issue also includes a new version of the checklist and a red list of hornworts and liverworts which updates data published 20 years ago. The checklist is followed by a paper on new localities and phylogenetic relationships of Leucoglossum leucosporum, a geoglossoid fungus whose occurrence is poorly known. The last two contributions are devoted to the impact of the environmental burdens on biota.