Analogue-specific action in vitro of atrial natriuretic factor on human red blood cell Ca2+-ATPase activity.

Abstract

SUMMARY Specific atrial natriuretic factor (ANF) analogues have been found to have inhibitory activity in vitro in a calmodolin-dependent, human red blood cell membrane Ca 2+ -adeoosine triphosphatase (ATPase) model. Studied at 10"' to 10"' M concentrations, atriopeptin I (residues 127-147 of rat prepro-ANF sequence) and atriopeptin i n (residues 127-150) progressively inhibited Ca A TRIAL natriuretic factor (ANF) has natri-Z A uretic' and blood pre ssure-lowering 2 actions J.Ain intact animals and has also been shown to inhibit the release of renin 3 and aldosterone. 4 The diverse effects of ANF also include stimulation of arginine vasopressin secretion 5 and of renal gluconeogenesis. 6 The mechanisms of ANF actions are incompletely understood, but they are thought to involve enhanced guanylate cyclase activity (e.g., in the kidney) 7 and, perhaps, lowering of intracellular Ca 2+ concentrations. 8 On the other hand, the renal tubular gluconeogenic action of ANF is Ca 2+ -dependent, 6 and gluconeogenesis in the kid-