Analogs of the Cathelicidin-Derived Antimicrobial Peptide PMAP-23 Exhibit Improved Stability and Antibacterial Activity

Liu, Yongqing; Shen, Tengfei; Chen, Liangliang; Zhou, Jiangfei; Wang, Chen

doi:10.1007/s12602-020-09686-z

Cited by 13 publications

(9 citation statements)

References 43 publications

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“…In addition, the cathelicidin-derived antimicrobial peptide PMAP-23 was further modified by amino acid substitutions to obtain PMAP-23RI (for, Leu5-Arg and Thr19-Ile). The variant exhibited a higher stability and improved antibacterial activity when compared with the original PMAP-23, with MICs reduced by half in some cases from a simple modification to the original AMP [132]. Analogs of mastoparan-C were also designed by amino acid substitution and peptide truncation, which reduced cell toxicity and improved the bioactivity of the original AMP [127,133].…”

Section: Synthetic Ampsmentioning

confidence: 99%

Antimicrobial Peptides: A Potent Alternative to Antibiotics

et al. 2021

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Antimicrobial peptides constitute one of the most promising alternatives to antibiotics since they could be used to treat bacterial infections, especially those caused by multidrug-resistant pathogens. Many antimicrobial peptides, with various activity spectra and mechanisms of actions, have been described. This review focuses on their use against ESKAPE bacteria, especially in biofilm treatments, their synergistic activity, and their application as prophylactic agents. Limitations and challenges restricting therapeutic applications are highlighted, and solutions for each challenge are evaluated to analyze whether antimicrobial peptides could replace antibiotics in the near future.

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Section: Synthetic Ampsmentioning

confidence: 99%

Antimicrobial Peptides: A Potent Alternative to Antibiotics

et al. 2021

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“…PMAP-23 is a porcine antimicrobial peptide discovered in 1994 ( Zanetti et al., 1994 ), which is rich in arginine and contains 2 α-helical regions. The C-terminal is lipophilic, can be inserted into cells to break cells, and has anti-Gram-positive and Gram-negative bacteria ( Liu et al., 2020 ).…”

Section: Mucosal Immune System Regulates Intestinal Microbiotamentioning

confidence: 99%

Gut health: The results of microbial and mucosal immune interactions in pigs

2021

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There are a large number of microorganisms in the porcine intestinal tract. These microorganisms and their metabolites contribute to intestinal mucosal immunity, which is of great importance to the health of the host. The host immune system can regulate the distribution and composition of intestinal microorganisms and regulate the homeostasis of intestinal flora by secreting a variety of immune effector factors, such as mucin, secretory immunoglobulin A (sIgA), regenerating islet-derived III (RegIII)γ, and defensin. Conversely, intestinal microorganisms can also promote the differentiation of immune cells including regulatory T cells (T reg ) and Th17 cells through their specific components or metabolites. Studies have shown that imbalances in the intestinal flora can lead to bacterial translocation and compromised intestinal barrier function, affecting the health of the body. This review focuses on the composition of the pig intestinal flora and the characteristics of intestinal mucosal immunity, discusses the interaction mechanism between the flora and intestinal mucosal immunity, as well as the regulation through fecal microbiota transplantation (FMT), dietary nutritional composition, probiotics and prebiotics of pig intestinal microecology. Finally, this review provides insights into the relationship between intestinal microorganisms and the mucosal immune system.

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“…In recent years, our group has performed research on the structural modification and antimicrobial activity of PMAPs. According to the structure and mechanism of PMAPs, many scholars have designed multiple novel modified peptides with increased stability and antimicrobial activity (43)(44)(45)(46)(47)(48)(49)(50). This review mainly summarizes the research progress on the structural characteristics and biological activities of PMAPs in recent years.…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

“…The fifth amino acid (leucine) of the PMAP-23-derived peptides, PMAP-23R (Leu5-Arg), PMAP-23I (Thr19-Ile), and PMAP-23RI (Leu5-Arg and Thr19-Ile), is substituted with an arginine to increase the net charge, and the nineteenth amino acid (threonine) of PMAP-23 is substituted with an isoleucine to increase the hydrophobicity (43). Both the antimicrobial spectrum and activity of PMAP-23 are significantly increased, especially against Gram-negative bacteria.…”

Section: Antibacterial Activity Of Pmap-23mentioning

confidence: 99%

Porcine Myeloid Antimicrobial Peptides: A Review of the Activity and Latest Advances

et al. 2021

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Traditional antibiotics have made great contributions to human health and animal husbandry since the discovery of penicillin in 1928, but bacterial resistance and drug residues are growing threats to global public health due to the long-term uncontrolled application of antibiotics. There is a critical need to develop new antimicrobial drugs to replace antibiotics. Antimicrobial peptides (AMPs) are distributed in all kingdoms of life, presenting activity against pathogens as well as anticancer, anti-inflammatory, and immunomodulatory activities; consequently, they have prospects as new potential alternatives to antibiotics. Porcine myeloid antimicrobial peptides (PMAPs), the porcine cathelicidin family of AMPs, have been reported in the literature in recent years. PMAPs have become an important research topic due to their strong antibacterial activity. This review focuses on the universal trends in the biochemical parameters, structural characteristics and biological activities of PMAPs.

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Analogs of the Cathelicidin-Derived Antimicrobial Peptide PMAP-23 Exhibit Improved Stability and Antibacterial Activity

Cited by 13 publications

References 43 publications

Antimicrobial Peptides: A Potent Alternative to Antibiotics

Antimicrobial Peptides: A Potent Alternative to Antibiotics

Gut health: The results of microbial and mucosal immune interactions in pigs

Porcine Myeloid Antimicrobial Peptides: A Review of the Activity and Latest Advances

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