Porcine Myeloid Antimicrobial Peptides: A Review of the Activity and Latest Advances

Shi, Shuaibing; Shen, Tengfei; Liu, Yongqing; Chen, Liangliang; Wang, Chen; Liao, Chengshui

doi:10.3389/fvets.2021.664139

Cited by 11 publications

(8 citation statements)

References 97 publications

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“…The research confirmed that broiler chickens have got an increase in body weight gain and feed performance in continuous heat stress conditions. 71 …”

Section: Antimicrobial Peptides: Multidrug-resistant Microbial Therap...mentioning

confidence: 99%

“…The research confirmed that broiler chickens have got an increase in body weight gain and feed performance in continuous heat stress conditions. 71 The Frog caerin, European sea bass dicentracin and NK-lysine peptides (NKLPs) have good inhibitory outcomes on Nodavirus, Septicaemic haemorrhagic virus, Infectious pancreatic necrosis virus and Spring viremia carp virus, which can be devastating to fish farming. The AMP in soybean meal fermented via Bacillus subtilis E20 successfully inhibits Bovine Neutrophil β-Defensins (BNBD) S. aureus [25] Swine SIAMP IBV in chickens [26] Protegrins (PG-1) P. aeruginosa, Chlamydia trachomatis, Neisseria gonorrhoeae [25] Proline-Arginine-39 (PR-39) Enterococcus faecalis, E. coli, and Bacillus subtilis [24] Sheep SBD-1 Variety of G. positive and G. negative bacteria [27] Equine Equine α and β Defensin Candida albicans, several G. positive and G. negative bacteria [25] Fish Hepcidins (LEAP-2)…”

Section: Antimicrobial Peptides Clinical Applications In Veterinary P...mentioning

confidence: 99%

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Multidrug-Resistant Microbial Therapy Using Antimicrobial Peptides and the CRISPR/Cas9 System

Getahun¹,

Ali²,

Taye³

et al. 2022

VMRR

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The emergence and spread of multidrug-resistant microbes become a serious threat to animal and human health globally because of their less responsiveness to conventional antimicrobial therapy. Multidrug-resistant microbial infection poses higher morbidity and mortality rate with significant economic losses. Currently, antimicrobial peptides and the CRISPR/Cas9 system are explored as alternative therapy to circumvent the challenges of multidrug-resistant organisms. Antimicrobial peptides are small molecular weight, cationic peptides extracted from all living organisms. It is a promising drug candidate for the treatment of multidrug-resistant microbes by direct microbial killing or indirectly modulating the innate immune system. The CRISPR/Cas9 system is another novel antimicrobial alternative used to manage multidrug-resistant microbial infection. It is a versatile gene-editing tool that uses engineered single guide RNA for targeted gene recognition and the Cas9 enzyme for the destruction of target nucleic acids. Both the CRISPR/Cas9 system and antimicrobial peptides were used to successfully treat nosocomial infections caused by ESKAPE pathogens, which developed resistance to various antimicrobials. Despite, their valuable roles in multidrug-resistant microbial treatments, both the antimicrobial peptides and the CRISPR/Cas systems have various limitations like toxicity, instability, and incurring high manufacturing costs. Thus, this review paper gives detailed explanations of the roles of the CRISPR/Cas9 system and antimicrobial peptides in circumventing the challenges of multidrug-resistant microbial infections, its limitation and prospects in clinical applications.

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“…The research confirmed that broiler chickens have got an increase in body weight gain and feed performance in continuous heat stress conditions. 71 …”

Section: Antimicrobial Peptides: Multidrug-resistant Microbial Therap...mentioning

confidence: 99%

Section: Antimicrobial Peptides Clinical Applications In Veterinary P...mentioning

confidence: 99%

Multidrug-Resistant Microbial Therapy Using Antimicrobial Peptides and the CRISPR/Cas9 System

Getahun¹,

Ali²,

Taye³

et al. 2022

VMRR

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show abstract

“…Antimicrobial peptides (AMPs) are a potential alternative to antibiotics due to their broad-spectrum antimicrobial activities and low drug resistance ( Zhang et al, 2019 ), and they exhibit broad biomedical application prospects on account of their anticancer, antiviral, antiparasitic, and immunomodulatory effects ( Shi et al, 2021 ). According to the site of action, AMPs are divided into two categories: membrane and non-membrane disruption AMPs.…”

Section: Introductionmentioning

confidence: 99%

Prediction and bioactivity of small-molecule antimicrobial peptides from Protaetia brevitarsis Lewis larvae

Cao

Sun

et al. 2023

Front. Microbiol.

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Antimicrobial peptides (AMPs) are widely recognized as promising natural antimicrobial agents. Insects, as the group of animals with the largest population, have great potential as a source of AMPs. Thus, it is worthwhile to investigate potential novel AMPs from Protaetia brevitarsis Lewis larvae, which is a saprophagous pest prevalent in China. In this study, comparing the whole-genome sequence of Protaetia brevitarsis Lewis larvae with the Antimicrobial Peptide Database (APD3) led to the identification of nine peptide templates that were potentially AMPs. Next, based on the peptide templates, 16 truncated sequences were predicted to the AMPs by bioinformatics software and then underwent structural and physicochemical property analysis. Thereafter, candidate small-molecule AMPs were artificially synthesized and their minimal inhibitory concentration (MIC) values were assessed. A candidate peptide, designated FD10, exhibited strong antimicrobial activity against both bacteria and fungi comprising Escherichia coli (MIC: 8 μg/mL), Pseudomonas aeruginosa (MIC: 8 μg/mL), Bacillus thuringiensis (MIC: 8 μg/mL), Staphylococcus aureus (MIC: 16 μg/mL), and Candida albicans (MIC: 16 μg/mL). Additionally, two other candidate peptides, designated FD12 and FD15, exhibited antimicrobial activity against both E. coli (MIC: both 32 μg/mL) and S. aureus (MIC: both 16 μg/mL). Moreover, FD10, FD12, and FD15 killed almost all E. coli and S. aureus cells within 1 h, and the hemolytic effect of FD10 (0.31%) and FD12 (0.40%) was lower than that of ampicillin (0.52%). These findings indicate that FD12, FD15, and especially FD10 are promising AMPs for therapeutic application. This study promoted the development of antibacterial drugs and provided a theoretical basis for promoting the practical application of antimicrobial peptides in the Protaetia brevitarsis Lewis larvae.

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“…Porcine myeloid antimicrobial peptides (PMAPs) are the cathelicidin family of AMPs derived from porcine bone marrow cells with stable α-helix structures and have been reported to possess high antibacterial, antifungal, antitumor, and antiparasitic activity [ 8 ]. PMAPs hold enormous promise to develop stable, safe, and effective antimicrobial drugs.…”

Section: Introductionmentioning

confidence: 99%

Sustained release of alginate hydrogel containing antimicrobial peptide Chol-37(F34-R) in vitro and its effect on wound healing in murine model of Pseudomonas aeruginosa infection

Shi,

Dong,

Chen

et al. 2023

J Vet Sci

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Background Antibiotic resistance is a significant public health concern around the globe. Antimicrobial peptides exhibit broad-spectrum and efficient antibacterial activity with an added advantage of low drug resistance. The higher water content and 3D network structure of the hydrogels are beneficial for maintaining antimicrobial peptide activity and help to prevent degradation. The antimicrobial peptide released from hydrogels also hasten the local wound healing by promoting epithelial tissue regeneration and granulation tissue formation. Objective This study aimed at developing sodium alginate based hydrogel loaded with a novel antimicrobial peptide Chol-37(F34-R) and to investigate the characteristics in vitro and in vivo as an alternative antibacterial wound dressing to treat infectious wounds. Methods Hydrogels were developed and optimized by varying the concentrations of crosslinkers and subjected to various characterization tests like cross-sectional morphology, swelling index, percent water contents, water retention ratio, drug release and antibacterial activity in vitro , and Pseudomonas aeruginosa infected wound mice model in vivo . Results The results indicated that the hydrogel C proved superior in terms of cross-sectional morphology having uniformly sized interconnected pores, a good swelling index, with the capacity to retain a higher quantity of water. Furthermore, the optimized hydrogel has been found to exert a significant antimicrobial activity against bacteria and was also found to prevent bacterial infiltration into the wound site due to forming an impermeable barrier between the wound bed and external environment. The optimized hydrogel was found to significantly hasten skin regeneration in animal models when compared to other treatments in addition to strong inhibitory effect on the release of pro-inflammatory cytokines (interleukin-1β and tumor necrosis factor-α). Conclusions Our results suggest that sodium alginate -based hydrogels loaded with Chol-37(F34-R) hold the potential to be used as an alternative to conventional antibiotics in treating infectious skin wounds.

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Porcine Myeloid Antimicrobial Peptides: A Review of the Activity and Latest Advances

Cited by 11 publications

References 97 publications

Multidrug-Resistant Microbial Therapy Using Antimicrobial Peptides and the CRISPR/Cas9 System

Multidrug-Resistant Microbial Therapy Using Antimicrobial Peptides and the CRISPR/Cas9 System

Prediction and bioactivity of small-molecule antimicrobial peptides from Protaetia brevitarsis Lewis larvae

Sustained release of alginate hydrogel containing antimicrobial peptide Chol-37(F34-R) in vitro and its effect on wound healing in murine model of Pseudomonas aeruginosa infection

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