ANALISIS RESIDU ANTIBIOTIK TETRASIKLIN PADA DAGING AYAM BROIlER DAN DAGING SAPI

Abstract

Tetracycline are antibiotics with broad-spectrum that used routinely in veterinary medicine for animal treatment and prevention from infectious diseases. But, consuming antibiotics for long time and exceeding standards can lead to accumulation of antibiotic residues in the body. Based on SNI 01-6366-2000, the maximum residual limit (BMR) of tetracycline antibiotic is 0.1 mg/kg in meat.The purpose of this study was to determine the concentration of tetracycline residues in broiler chicken meat and beef that obtained in East Surabaya namely Manyar market, Sinar Baru market, Ampengan Batu market, Tenggilis Mejoyo market, Krempyeng market, Soponyono market, and Gunung Anyar market. This study used Thin Layer Chromatography (TLC) as qualitative test and Spectrophotometric method as a quantitative test to determine the residual levels of tetracycline antibiotics. This study was conducted on March 15 onto May 2 2019. Based on the study, about 32 broiler chicken and 13 beef samples detected by the TLC method showed that had negative results, there was no containing tetracycline residues in samples. Awhile the results of the spectrophotometric method showed that as many as 31 samples of broiler chicken and 13 beef samples showed that had positive results containing residues of tetracycline antibiotics that exceed of the standard. Based on the results obtained that average concentration of tetracycline in samples of broiler chicken meat about 1,7 mg/kg and 0,8 mg/kg in beef samples. Descriptively,tetracycline residues in broiler chicken meat were higher than beef.

Deteksi Residu Antibiotik pada Daging Ayam Broiler dan Itik Serta Tingkat Kesadaran Peternak di Wilayah Selemadeg Timur Tabanan Bali

“…Hal ini sesuai dengan penelitian Nadzifah (2019) yang menyatakan bahwa tidak ditemukannya keberadaan residu tetrasiklin pada daging broiler, karena peternak broiler telah mengerti penggunaan antibiotik sesuai dengan masa henti obat (withdrawal time) dan dosis yang tepat (Nadzifah et al, 2019). Penelitian lain dari Aniza et al (2019) juga menjelaskan bahwa 32 sampel daging ayam dan 13 sampel daging sapi negatif residu antibiotik tetrasiklin menggunakan metode Thin Layer Chromatography (TLC) (Aniza et al, pISSN: 2085-2495eISSN: 2477eISSN: -2712 Desember 2022 Online pada: http://ojs.unud.ac.id/index.php/buletinvet DOI: 10.24843/bulvet.2022.v14.i06.p17 2019). Kemungkinan pertama ini telah didukung dengan dengan hasil kuesioner peternak pada tabel 5.2.1 dimana tingkat pengetahuan peternak terhadap penggunaan antibiotik meningkat setelah diterbitkannya UU.…”

“…Menurut Aniza et al (2019), pakan yang mengandung antibiotik jika diberikan pada unggas akan berinteraksi dengan jaringan (organ). Jika pakan yang mengandung antibiotik dalam jumlah yang kecil, pengaruh yang ditimbulkan tidak akan secara langsung namun akan berefek kronis dan tetap berada dalam tubuh unggas.…”

Lactobacillus Substitution as a Chicken Antibiotic Growth Promotor: A Systematic Review

“…The most visible antibiotic residues were in chickens because antibiotics were used as a treatment method, infection in chickens without controlling the dose and use of antibiotics, and antibiotics as feed additives in feed. Research showed excessive doses of antibiotic residues in chicken farms and markets were easily found [8,11,12]. The common reasons for tetracycline usage were easy to use and cheap.…”

“…However, the rest of these compounds will still be stored in the organs, called residues. When the feed is mixed with antibiotics for a long time, the antibiotic residues in the organs will accumulate in the organs with varying concentrations [12].…”

Kajian Residu Tetrasiklin HCl dalam Daging dan Hati Ayam Broiler pada Beberapa Peternakan di Kabupaten Lamongan Menggunakan Metode Spektrofotometri Ultraviolet

“…Terlebih jika residu masih menyisakan aktivitas antibakteri maka dapat menyebabkan resistensi antibiotik [5] . Berdasarkan penelitian yang telah dilakukan, residu antibiotik banyak ditemukan pada organ hati, ginjal, dan daging ayam broiler [6] .…”