Análise imuno-histoquímica de cães naturalmente infectados pelo parvovírus canino

Oliveira, Eduardo Conceição de; Pescador, Caroline Argenta; Sonne, Luciana; Pavarini, Saulo Petinatti; Santos, Adriana da Silva; Corbellini, Luís Gustavo; Driemeier, David

doi:10.1590/s0100-736x2009000200008

Cited by 11 publications

(10 citation statements)

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“…A Diocrophyme renale specimen was also found in the abdominal cavity of this animal. The clinical signs of parvovirus infection and lesions were similar to those found in domestic canids affected by this disease (Oliveira et al 2009). The otter analyzed in this study was rescued in the urban area of the municipality of Pelotas, which has a population of approximately 66,700 canids, and approximately 80% of them are semi-domiciled or stray (Pelotas 2012).…”

Section: Discussionsupporting

confidence: 57%

Lontra longicaudis infected with canine parvovirus and parasitized by Dioctophyma renale

et al. 2018

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This study describes a case of parvovirus infection in a river otter (Lontra longicaudis) assisted at the Wildlife Rehabilitation Center and Wildlife Screening Center, Federal University of Pelotas (UFPel), Rio Grande do Sul state, Brazil. Clinical signs included apathy, dark and fetid diarrhea, and crusted lesions on the palmar pads of the fore and hind limbs. The animal died after undergoing support treatment with antibiotics, anti-inflammatory, and fluid therapy. At necropsy, the intestines were reddened and edematous and the right kidney was diminished by one third of its normal size and covered with whitish, spongy material. A female Dioctophyma renale was found free in the abdominal cavity. Histologically, dilatation of the intestinal crypts and fusion and blunting of the intestinal villi were observed. In addition, moderate, multifocal lymphocytic enteritis with lymphoid depletion in Peyer’s patches and mesenteric lymph nodes were present. Immunohistochemistry with anti-canine parvovirus monoclonal antibody (anti-CPV) was strongly positive in the bone marrow cells and enterocytes of the intestinal crypts, confirming the diagnosis of parvovirus infection. The peritoneum on the right kidney was expanded with a cuboidal cell border, forming multiple papillary projections associated with eggs of D. renale and severe inflammatory infiltrate (giant cells, macrophages, lymphocytes, eosinophils, and plasma cells). Areas of necrosis and mineralization were also observed. Due to fragmentation and degradation of its natural habitat, the otter approached the urban area and was contaminated with the virus, which is hosted and disseminated by domestic animals. Infection with D. renale can be associated with the large population of parasitized domestic animals, which eliminate the helminth eggs through urine, contaminating the environment where the parasite intermediate and paratenic hosts co-inhabit. The diseases of these animals can be a decline factor of wild populations that inhabit the region and are an alert to spillover risk.

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Section: Discussionsupporting

confidence: 57%

Lontra longicaudis infected with canine parvovirus and parasitized by Dioctophyma renale

et al. 2018

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“…Fatores como tempo de fixação/armazenamento na solução de formol maior que dois dias (Werner et al 2000); o fato da IHQ ser um teste altamente específico, porém de menor sensibilidade (Van Maanen et al 2004) e rápida redução da carga viral tecidual (Carman & Povey 1985) são possibilidades para a ocorrência de resultados negativos na IHQ. A escassez de trabalhos com uso de IHQ em casos de panleucopenia felina permitiu comparar os resultados apenas com a utilização do exame no diagnóstico da parvovirose canina, no qual foi também demonstrado que o intestino delgado é o órgão que apresenta melhor imunomarcação, com 77,1% de marcações positivas (Oliveira et al 2009). Em trabalhos experimentais sobre parvovirose canina descreve-se que o vírus é detectado primeiro em tonsilas, linfonodos retrofaríngeos e mesentéricos, no primeiro e no segundo dia após inoculação (Meunier et al 1985), e não pode ser encontrado no intestino delgado antes de quatro ou cinco dias após inoculação (Carman & Povey 1985, Meunier et al 1985) e com possibilidade de detecção por IHQ no epitélio intestinal até oito dias após a inoculação viral (Carman & Povey 1985).…”

Section: Discussionunclassified

“…Esse resultado pode ser explicado pela distribuição e pela concentração viral em determinado período; após o sexto dia de inoculação a quantidade de vírus circulante diminui consideravelmente, com menor marcação em tecidos como timo, linfonodos mesentéricos e baço (Meunier et al 1985). Resultado semelhante foi encontrado na utilização dessa técnica em cães com parvovirose, em que órgãos linfoides apresentavam marcação menos intensa e com menor número de cortes positivos (Oliveira et al 2009).…”

Section: Discussionunclassified

“…O uso da língua na IHQ de parvovírus de cães e gatos foi utilizada em outros estudos pela melhor conservação e pela possibilidade de elevada taxa de replicação viral nas células do epitélio escamoso estratificado (McKnight et al 2007, Oliveira et al 2009). Entretanto pelo baixo número casos testados, a eficácia da utilização da língua no diagnóstico imuno-histoquímico da panleucopenia deve ser confirmada em novas pesquisas.…”

Section: Discussionunclassified

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Achados patológicos e imuno-histoquímicos em felinos domésticos com panleucopenia felina

et al. 2014

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Feline panleukopenia is an important infectocontagious disease of domestic feline, especially in animals under 1 year. This paper describes the clinical-pathological findings and the immunohistochemical diagnosis of 33 cases of feline panleukopenia. The most important clinical signs were vomiting, diarrhea, and anorexia. The main gross findings observed were reddening of intestinal mucosa (16/33), evidentiation of Peyer patches (14/33), and liquefied intestinal content (7/33). The most consistent histological findings were necrosis (33/33) and lymphohistiocytic inflammatory infiltrate in the intestinal mucosa (31/33), villus fusion (27/33) and villus atrophy (26/33). In the hematopoietic tissues, the findings were characterized mainly by necrosis and tissue depletion. Parvovirus positive immunohistochemichal results were obtained in 84.85% of the cases analyzed. The best organ for viral detection was the intestine, with 84.85% of labeling in the immunohistochemichal technique. The spleen showed the best result among lymphoid organs, with 47.37% of the sections positive. This study presents most important lesions in the small intestine and in lymphoid organs and the immunohistochemistry proved good results in the detection of parvovirus.

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“…The sections were counterstained with hematoxylin and analyzed under an optical microscope. Positive controls from previously tested positive cases (OLIVEIRA et al, 2009) were also used.…”

Section: Immunohistochemical Assaysmentioning

confidence: 99%

Immunohistochemical and serological aspects of Toxoplasma gondii infection in Neotropical primates

Grumann

Silva

Filho

et al. 2017

SCA

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Toxoplasmosis, a disease caused by the intracellular coccidian Toxoplasma gondii that infects most warm-blooded vertebrates, is widely distributed and fatal for primates, which are peculiarly susceptible for unknown reason(s). Owing to the increasing number of Neotropical mammal deaths where in T. gondii were detected in analyzed tissues, the present immunohistochemical study analyzed the distribution patterns of immunostainings related to this parasite on primates necropsied at the Laboratório de Patologia Animal of Universidade de Passo Fundo (UPF), between the years of 2000 and 2014. Furthermore, a serological survey for the disease was conducted for 21 primates from the UPF Zoo, Rio Grande do Sul, Brazil, belonging to genera Sapajus and Alouatta. In a immunohistochemical test performed using streptavidin-biotin-peroxidase, 26.53% positivity was detected in 98 primates. Immunostainings revealed that infection differed among the lung (76.92%), liver (58.33%), heart (50%), brain (42.30%), and kidney (23.07%) tissues. Serology performed through indirect hemagglutination showed reactivity in 85.7% of the animals, all belonging to Sapajus sp., while the three primates that did not show reactivity (14.3%) belonged to Alouatta sp.

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Análise imuno-histoquímica de cães naturalmente infectados pelo parvovírus canino

Cited by 11 publications

References 12 publications

Lontra longicaudis infected with canine parvovirus and parasitized by Dioctophyma renale

Lontra longicaudis infected with canine parvovirus and parasitized by Dioctophyma renale

Achados patológicos e imuno-histoquímicos em felinos domésticos com panleucopenia felina

Immunohistochemical and serological aspects of Toxoplasma gondii infection in Neotropical primates

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