Analgesic effects of astilbin partially via calcium channels through regulation on CaMKII

Abstract

This study aimed to evaluate the analgesic efficacy of astilbin and its underlying mechanisms. Astilbin suppressed the symptoms of acetic acid-induced writhing, lengthened the latency period on the hot plate, and reduced the licking and biting response of formalin-injected mice, suggesting its analgesic efficacy on the central nervous system. Astilbin suppressed neuronal nitric oxide synthase and enhanced serotonin and norepinephrine in serum and brains of mice after 30 s of thermal stimulation, but it failed to influence their levels before thermal stimulation. Among six chosen antagonists, only nimodipine, a calcium channel blocker, strongly enhanced the abirritation of astilbin, as indicated by the lengthened latency period of mice treated with nimodipine plus astilbin in the hot plate test. The expression levels of c-Fos and phosphorylated calmodulin-dependent protein kinase II and c-Jun N-terminal kinase in the brains were reduced in the astilbintreated mice. Astilbin-mediated analgesia is partially related to Ca 2+ channels. ARTICLE HISTORY

Bisbenzylisoquinoline alkaloids of lotus ( Nelumbo nucifera Gaertn.) seed embryo inhibit lipopolysaccharide-induced macrophage activation via suppression of Ca 2+ -CaM/CaMKII pathway

“…CaM is the critical effector of Ca 2+ . Furthermore, CaMKII, one of the main downstream targets of Ca 2+ and CaM, is activated by Ca 2+ /CaM (Bi et al, 2019;Chin & Means, 2000;Ohmori & Hamilton, 1992). To further elucidate the role of CaM and CaMKII in the inhibition of macrophage activation by three alkaloids, the expression of CaM, phosphorylated-CaMKII and total CaMKII was detected by western blot assay.…”

Microwave-assisted extraction and content determination of astilbin in Lysiphyllum strychnifolium stems

“…Astilbin (Figure 1), a flavonoid glycoside, is one compound recently isolated from L. strychnifolium (Sampaopan et al, 2021), specifically found in extracts (Bi et al, 2019;Lu et al, 2015). The astilbin is separated from the extracts by column chromatography (Merck silica gel 60, 70-230 mesh) eluted with ethyl acetate and methanol (95: 5, %v/v), and reversed-phase column chromatography (Merck LiChroprep RP-18, 40-63 µm) eluted with methanol and water (50: 50, % v/v).…”