Analgesic and antiinflammatory activities of the ethyl acetate fraction of Bidens pilosa (Asteraceae)

Fotso, Aurélien Fotso; Longo, Flavia; Djomeni, Paul Désiré Dzeufiet; Kouam, Siméon F.; Spiteller, Michael; Dongmo, Alain Bertrand; Savineau, Jean‐Pierre

doi:10.1007/s10787-013-0196-2

Cited by 34 publications

(20 citation statements)

References 27 publications

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“…Fresh leaves of Bidens pilosa L (Asteraceae) were collected from Yaounde’s suburb (Eleveur) on September 2011 and authenticated at the National Herbarium of Cameroon by comparison to the voucher specimen (No 65112/HNC) deposited in 2005. The extraction was done as previously described [ 15 ]. Briefly, the ethyl acetate extract was prepared by macerating 1000 g of air dried leaves for two days in 5 L of methylene chloride/methanol (1:1).…”

Section: Methodsmentioning

confidence: 99%

“…Bidens pilosa is now knowm to contain several chemical and useful compounds, including at least 60 flavonoids [ 7 ]. In the present plant extract, we identified two bioactive flavonoids namely Quercetin 3,3 ‘-dimethyl ether 7–0-β-D-glucopyranoside and Iso-Okanin 7-O-β-D-(2 “, 4″, 6 “-triacetyl)-glucopyranoside [ 15 ]. Quercetin is known to improve vascular function in an endothelium-dependant and -independent manner [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Bidens pilosa Ethylene acetate extract can protect against L-NAME-induced hypertension on rats

Bilanda

Dzeufiet

Kouakep

et al. 2017

BMC Complement Altern Med

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BackgroundEssential hypertension is mainly caused by endothelial dysfunction which results from nitric oxide (NO) deficiency. The present study was design to evaluate the protective effect of Bidens pilosa ethylene acetate extract (Bp) on L-NAME induced hypertension and oxidative stress in rats.MethodsMale Wistar rats were used to induce hypertension by the administration of L-NAME (a non-pecific nitric oxide inhibitor) (50 mg/kg/day). The others groups were receiving concomitantly L-NAME plus Bp extract (75 and 150 mg/kg/day) or losartan (25 mg/kg/day). All the treatments were given orally for 4 weeks. At the end of the treatment, the hemodynamic parameters were recorded using the direct cannulation method. The effects of the extract on lipid profile, kidney and liver functions as well as oxidative stress markers were evaluated by colorimetric method. Results were expressed as the mean ± SEM. The difference between the groups was compared using one-way analysis of variance (ANOVA) followed by the Duncan’s post hoc test.ResultsAnimals receiving L-NAME presented high blood pressure, normal heart rate and lipid profile as well as NO depletion, liver and kidney injuries and oxidative stress. The concomitant treatment with L-NAME and Bp or losartan succeeded to prevent the raised of blood pressure and all the other injuries without affecting the heart rate.ConclusionThese results confirm the antihypertensive effects of Bidens pilosa and highlight its protective properties in L-NAME model of hypertension in rat, probably due to the presence of Quercetin 3,3 ‘-dimethyl ether 7–0-β-D-glucopyranoside.Electronic supplementary materialThe online version of this article (10.1186/s12906-017-1972-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bidens pilosa Ethylene acetate extract can protect against L-NAME-induced hypertension on rats

Bilanda

Dzeufiet

Kouakep

et al. 2017

BMC Complement Altern Med

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“…These results strongly suggest that isoquercitrin significantly inhibits the progression of bladder cancer in vivo. efficacies (18)(19)(20). Ma et al (21) and Zhong et al (22) utilized macroporous adsorptive resins and high-performance liquid chromatography (HPLC) to separate the main components of total flavones in Bidens and proved that these components mainly consist of isoquercitrin and hyperoside.…”

Section: Isoquercitrin-mediated Cell Cycle Arrest Of Bladder Cancer Cmentioning

confidence: 99%

Isoquercitrin inhibits bladder cancer progression in vivo and in vitro by regulating the PI3K/Akt and PKC signaling pathways

Chen

Yang

et al. 2016

Oncology Reports

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Bladder cancer is the most common malignancy of the urinary system and is also one of the 10 most common cancers of the human body. Currently, clinical treatment of bladder cancer mainly utilizes partial or total cystectomy, supplemented by conventional chemotherapy. However, such treatment has not fully improved the prognosis of patients and is associated with various side effects. Studies have found that flavonoids extracted from plants can be used in radiotherapy and chemotherapy for the prevention of postoperative recurrence and metastasis but also alone for the treatment of advanced tumors. Both applications can ameliorate clinical symptoms, improve the quality of life, and prolong the survival of patients. Based on the above information, the present study investigated the effect of isoquercitrin, a type of flavonoid found in Bidens pilosa L. extracts, on bladder cancer progression, with the goal of understanding the biological characteristics of isoquercitrin by which it participates in bladder cancer progression. Using in vitro experiments, we found that therapeutic doses of isoquercitrin significantly inhibited cell proliferation and induced apoptosis in human bladder cancer cells and that the cell cycle was arrested in the G1 phase. Isoquercitrin inhibited phosphatidylinositol 3-kinase (PI3K) and Akt phosphorylation expression levels, thus inhibiting proliferation and inducing apoptosis in the cancer cells. In addition, we found that isoquercitrin reduced protein kinase C (PKC) protein expression levels in the human bladder cancer cell lines. We also showed via in vivo experiments that isoquercitrin inhibited xenograft tumor growth in nude mice. In conclusion, our study confirmed that isoquercitrin inhibits bladder cancer progression in vivo and demonstrated that the molecular mechanism of this inhibition may be closely associated with the PI3K/Akt and PKC signaling pathways.

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“…The bioactive ingredients in the genus of Bidens are flavonoids and polyacetylenes (glycosylated or not). The pharmacological activity of Bidens pilosa L. is mainly attributed to these bioactive compounds . Thus, it is necessary to establish a valid strategy to explain the herb ingredients complexity.…”

Section: Introductionmentioning

confidence: 99%

Separation and identification of phenolic compounds in Bidens pilosa L. by ultra high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

Liang

2016

J of Separation Science

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A validated method based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was established to separate and identify phenolic compounds in Bidens pilosa L. Mass spectrometry experiments were performed both in positive and negative ion modes. A total of 35 compounds were detected, and 26 phenolic compounds were unequivocally identified or tentatively assigned based on retention time, maximum UV absorption, molecular formula, and fragments. The ultra high performance liquid chromatography method was validated and showed good linearity (R(2) ≧ 0.9996) over the test range. The limits of detection and quantification were above 0.072 and 0.162 μg/mL, respectively. The relative standard deviations of intraday and interday precision were below 0.3 and 1.6%, respectively.

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Analgesic and antiinflammatory activities of the ethyl acetate fraction of Bidens pilosa (Asteraceae)

Cited by 34 publications

References 27 publications

Bidens pilosa Ethylene acetate extract can protect against L-NAME-induced hypertension on rats

Bidens pilosa Ethylene acetate extract can protect against L-NAME-induced hypertension on rats

Isoquercitrin inhibits bladder cancer progression in vivo and in vitro by regulating the PI3K/Akt and PKC signaling pathways

Separation and identification of phenolic compounds in Bidens pilosa L. by ultra high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

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