Analgesic and anti-inflammatory effects and molecular mechanisms of Kadsura heteroclita stems, an anti-arthritic Chinese Tujia ethnomedicinal herb

Yu, Haichao; Lin, Yuanyuan; Zeng, Rong; Li, Xin; Zhang, Ting; Tasneem, Shumaila; Chen, Cong; Qiu, Yixing; Li, Bin; Liao, Jinfeng; Wang, Yuhong; Cai, Xiong; Wang, Wei

doi:10.1016/j.jep.2019.111902

Cited by 25 publications

(17 citation statements)

References 30 publications

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“…In this work, we evaluated the anti-inflammatory effects of GLY in two acute inflammation models, and evaluated the therapeutic effect of GLY in an ammoniainduced model of CP in rats. Xylene-induced mice ear edema and carrageenaninduced rat paw edema are currently the two most widely used models of inflammation and are an effective tool for the study of novel anti-inflammatory drugs [24,38,39]. Several of the herbs included in GLY, such as HQ, SH and MD can significantly inhibit ear and foot swelling in mice [40][41][42].…”

Section: Discussionmentioning

confidence: 99%

“…Xylene-induced mice ear edema and carrageenan-induced rat paw edema are currently the two most widely used models of inflammation and are an effective tool for the study of novel anti-inflammatory drugs [ 24 , 38 , 39 ]. Several of the herbs included in GLY, such as HQ, SH and MD can significantly inhibit ear and foot swelling in mice [ 40 – 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…A vernier caliper was used to measure the thickness of both hind paws of each animal at 1 h before induction and 1~6 h after induction. Calculating the increase rate that in paw thickness of the right hind paw (foot swelling) was calculated as: the increase rate = (B-A) / A × 100%, where A represents the paw thicknesses before induction and B represents the paw thicknesses at different time points after the induction [24].…”

Section: Feet Swelling Assaymentioning

confidence: 99%

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Anti-inflammatory effect of Ganluyin, a Chinese classic prescription, in chronic pharyngitis rat model

Chen

Luo

Lei

et al. 2020

BMC Complement Med Ther

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Background: Ganluyin (GLY) is a famous classical prescription with a long history of use as a treatment for inflammatory conditions such as chronic pharyngitis (CP) in many parts of China. However, it has not been developed as a modern pharmaceutic and its anti-inflammatory mechanisms remain unclear. The aim of this study was to assess the anti-inflammatory efficacy of GLY and potential mechanisms in a rat model of CP. Methods: The chemical profile of GLY was analyzed by HPLC-UV. We used a mouse model of ear edema and a rat model of paw edema. Specifically, xylene was used to induce edema on the surface of one ear in mice, and carrageenan was injected subcutaneously into the right hind paws of rats to induce paw edema. The paw thickness, ear weight, and ear perfusion were measured and recorded. The CP model in rats was induced by irritating the throat with 5% ammonia and was used to evaluate the therapeutic efficacy of GLY. Levels of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor (TNF-α), and prostaglandin E2 (PGE2) were measured by ELISA in serum, and protein expression of cyclooxygenase-2 (COX-2) and nuclear factor kappa-B p65 (NF-κB p65) in the throat were detected by immunohistochemistry and Western blot to evaluate the anti-inflammatory mechanism of GLY. Hematological assays were also conducted. Results: There were four flavonoids identified in GLY: naringin, neohesperidin, baicalin, and wogonoside. The oral administration of GLY showed a significant inhibitory effect on xylene-induced ear swelling and ear blood flow in mice and significantly ameliorated rat right hind paw edema at doses of 6.2 and 12.4 g/kg. Mechanistic studies found that the anti-inflammatory activity of GLY was related to the inhibition of pro-inflammatory cytokines such as IL-1β, IL-6, TNF-α, and PGE2 and that GLY reduced the expression of COX-2 and NF-κB p65 proteins in the throat, attenuated throat injury, and reduced inflammatory exudates. Hematological analysis showed that treatment with GLY prevented increases in white blood cell (WBC), neutrophil (NEUT), lymphocyte (LYMPH) and monocyte (MONO) levels.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Feet Swelling Assaymentioning

confidence: 99%

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Anti-inflammatory effect of Ganluyin, a Chinese classic prescription, in chronic pharyngitis rat model

Chen

Luo

Lei

et al. 2020

BMC Complement Med Ther

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“…COX has two isoforms, COX-1 and COX-2. COX-1 is constitutively expressed in virtually all tissues that are involved in physiological gastrointestinal, renal and cardiovascular activities [68]. Unlike COX-1, COX-2 is usually expressed at very low levels in most tissues under physiological conditions, but it is upregulated in inflammatory conditions [69].…”

Section: Discussionmentioning

confidence: 99%

Analgesic and antiinflammatory activities of the capilliposide derived from Lysimachia capillipes Hemsl., a traditional Chinese medicinal herb

Han

et al. 2020

ARCH BIOL SCI BELGRA

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Pain and inflammation are associated with the pathophysiology of different clinical conditions. The Lysimachia capillipes Hemsl. capilliposide (LCc) is the main bioactive component of this Chinese medicinal herb, which is widely used as a remedy for the treatment of colds and arthritis. This study investigated the analgesic and antiinflammatory activities of LCc in an animal model. LCc had no significant influence on the spleen, lung, liver and stomach coefficients in mice. Pharmacological studies showed that LCc at all doses (40, 60 and 90 mg/kg) increased the latency period of paw licking induced by thermal stimulation, and at the dose of 40 mg/kg it significantly suppressed abdominal writhing episodes of mice induced by intraperitoneal (i.p.) injection of acetic acid. LCc also had antiinflammatory effect on inflammation models. Doses of 60 and 90 mg/kg suppressed paw edema induced by subcutaneous (s.c.) injection of carrageenan. Mechanistic studies revealed that the antiinflammatory effect of LCc was associated with inhibition of the production of malondialdehyde (MDA), prostaglandin E2 (PGE2), tumor necrosis factor (TNF-α), cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) in paw tissue of carrageenan-injected mice. These results show that LCc has analgesic and antiinflammatory effects in mice.

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“…This was centrifuged at 4:C and 9,391 × g for 15 min, and the supernatant was collected. The levels of IL-1β, IL-6, and PGE2 were determined using ELISA kits according to the instructions provided by the manufacturer [17].…”

Section: Measurement Of Nomentioning

confidence: 99%

Analgesic and Anti-Inflammatory Activities of Sophocarpine from Sophora viciifolia Hance

Wang

et al. 2021

BioMed Research International

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Sophora viciifolia Hance is an edible plant used in traditional Chinese medicine. Sophocarpine, a tetracyclic quinolizidine alkaloid, is one of the most abundant active ingredients in Sophora viciifolia Hance. Here, we study the analgesic and anti-inflammatory effects, as well as the acute toxicity of sophocarpine from Sophora viciifolia Hance in mice. Sophocarpine (20, 40, and 80 mg/kgbw) significantly prolonged the delay period before a hot plate reaction occurred (all P < 0.05 ), and the delay before a tail-flick response was induced by a warm bath ( P < 0.05 ; P < 0.01 ). Sophocarpine (40, 80 mg/kg) resulted in dose-dependent inhibition of the writhing reaction induced by acetic acid in mice ( P < 0.05 ; P < 0.001 , respectively). Sophocarpine (80 mg/kg) reduced the total duration of a formalin-induced pain response ( P < 0.05 ). Sophocarpine prolonged the foot-licking latency of mice after the hot plate reaction, and this effect was antagonized by calcium chloride and enhanced by verapamil. Sophocarpine (20, 40, and 80 mg/kg) significantly inhibited xylene-induced ear edema ( P < 0.01 ; P < 0.001 ; P < 0.001 , respectively) and the penetration of acetic acid-induced dye into the peritoneal cavity ( P < 0.01 ; P < 0.01 ; P < 0.001 , respectively). It also reduced the levels of proinflammatory cytokine interleukin (IL)-1β, IL-6, and prostaglandin E2 ( P < 0.05 , P < 0.01 , P < 0.001 ) and those of serum nitric oxide ( P < 0.05 ). The results of this study suggest that sophocarpine possesses certain analgesic and anti-inflammatory activities, which may be related to calcium and inhibition of the secretion of inflammatory factors.

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Analgesic and anti-inflammatory effects and molecular mechanisms of Kadsura heteroclita stems, an anti-arthritic Chinese Tujia ethnomedicinal herb

Cited by 25 publications

References 30 publications

Anti-inflammatory effect of Ganluyin, a Chinese classic prescription, in chronic pharyngitis rat model

Anti-inflammatory effect of Ganluyin, a Chinese classic prescription, in chronic pharyngitis rat model

Analgesic and antiinflammatory activities of the capilliposide derived from Lysimachia capillipes Hemsl., a traditional Chinese medicinal herb

Analgesic and Anti-Inflammatory Activities of Sophocarpine from Sophora viciifolia Hance

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