Analgesic activity ofGleditsia triacanthosmethanolic fruit extract and its saponin-containing fraction

Abstract

Context: Gleditsia triacanthos L. (Leguminosae) pods are used in folk medicine for pain relief as anodyne and narcotic. Objective: The objective of this study is to evaluate analgesic activity of Gleditsia triacanthos methanolic fruit extract (MEGT) and its saponin-containing fraction (SFGT). Materials and methods: Peripheral analgesic activity was assessed using the acetic acid-induced writhing model in mice at doses of 140, 280, and 560 mg/kg and formalin test in rats at 100, 200, and 400 mg/kg doses. Central analgesic activity was evaluated using the hotplate method in rats (100, 200, and 400 mg/kg). Results: In the writhing test, six mice groups treated with MEGT and SFGT found ED 50 values 268.2 and 161.2 mg/kg, respectively, displayed a significant decrease in writhing count compared with the group treated with standard drug indomethacin (14 mg/kg). SFGT (280 and 560 mg/kg) showed 64.94 and 70.78% protection, respectively, which are more than double % protection caused by indomethacin (31.82%). In the formalin test, MEGT and SFGT (ED 50 values 287.6 and 283.4 mg/kg for phase I as well as 295.1 and 290.4 mg/kg for phase II, respectively) at 400 mg/kg showed significant % inhibition in both phase I (18.86 and 52.57%) and phase II (39.36 and 44.29%) with reference to 10 mg/kg indomethacin (56.0 and 32.29%). MEGT and SFGT caused significant delay in responses in hotplate model (ED 50 values 155.4 and 200.6 mg/kg, respectively) compared with that of 10 mg/kg indomethacin at 30, 60, and 120 min. Discussion and conclusion: Central and peripheral analgesic activities induced by Gleditsia triacanthos fruits might account for its uses in folk medicine.

Blue honeysuckle (Lonicera caerulea subsp. edulis (Turcz. ex Herder) Hultén.) berries and changes in their ingredients across different locations

“…Fruits with high saponin values also include black plum ( Syzygium cumini L.), papaya ( Carica papaya L.), yambo ( Syzygium yambos Alston), dragon fruit ( Hylocereus undatus Haworth.) and honey locust ( Gleditsia species) . Yassin et al .…”

Antinociceptive activity of petroleum ether fraction obtained from methanolic extract of Clinacanthus nutans leaves involves the activation of opioid receptors and NO-mediated/cGMP-independent pathway

“…Concurrently, the qualitative phytochemical analysis of PECN also showed the presence of flavonoids, saponins, steroids and triterpenes. Interestingly, various reports have shown that flavonoids [40, 41], triterpenes [42, 43], and saponins [44, 45] exert antinociceptive activity, which might explain the ability of PECN to induce antinociceptive activity.…”

Chemical Analysis, Antioxidant, Anti-Inflammatory and Antinociceptive Effects of Acetone Extract of Algerian Solenostemma Argel (Delile) Hayne Leaves

“…The presence of saponin derivatives in the acetone extract of S. argel leaves may also contribute to the observed analgesic effect. Indeed, several studies have demonstrated the analgesic effect of saponins on peripheral and central pain using the writhing test, the formalin test and the hot-plate test [62,[64][65][66]. Many saponins tested have displayed significant anti-nociceptive activity possibly due to their nonglycosidic moiety, the sapogenin [64].…”

“…Many saponins tested have displayed significant anti-nociceptive activity possibly due to their nonglycosidic moiety, the sapogenin [64]. The suggested mechanism involved in the analgesic activity of saponin is the blockage of the release of bradykinin, Prostaglandin, and cytokines [66].…”