Anal cytology in patients with AIDS

Calore, Edenilson Eduardo; Nadal, Sidney Roberto; Manzione, Carmen Ruth; Horta, S. C.; Santos, Ricardo Ribeiro dos; Nadal, Louis

doi:10.1002/dc.21201

Cited by 8 publications

(7 citation statements)

References 15 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…31 Cytologic abnormalities would be demonstrated even in patients without macroscopic condyloma in patients with AIDS. 32 In a study of the prevalence of abnormal anal cytology in women with lower genital tract intraepithelial neoplasia and cancer it was observed positive anal cytology in 9% of the patients but there was a high rate of anal HPV by analysis of HPV DNA (51%). 33 The difference of these results with ours would be due to technical procedures for collecting samples, as previously explained.…”

Section: Discussionmentioning

confidence: 96%

Prevalence of anal cytological abnormalities in women with positive cervical cytology

Calore

Giaccio

Nadal

2011

Diagnostic Cytopathology

View full text Add to dashboard Cite

The objective of this study was to estimate the prevalence of cytological abnormalities of the anal mucosa in women with positive cervical cytology, but without macroscopic anal lesion. Ultimately we postulated if the anal mucosa may be a reservoir of HPV, which would allow the reinfection of cervix. Forty-nine patients with abnormal cervical cytology were selected for this work. In a period not exceeding one week of collecting cervix cytology, two swab specimens of the anal canal were also collected. Women diagnosed with cervical HSIL by Pap smear were referred for colposcopy with biopsy of the lesions, to confirm the cytologic diagnosis and ablation of the lesion. We demonstrated a high prevalence of anal squamous intraepithelial lesions in patients with cervical squamous intraepithelial lesions (29 of the total of 49 patients = 59.2%). Of the 20 cases of cervical LSIL, 11 (55%) had abnormal anal cytology. Of the 26 cases with cervical HSIL, 16 (61.5%) had abnormal anal cytology. So, there was a discrete higher prevalence of abnormal anal cytology in cases of high-grade cervical squamous lesions (cervical HSIL). These results help to support the hypothesis that the anal mucosa is a reservoir of HPV, which can be a source of re-infection for the cervix. However, there was no significant association between the practice of anal sex and the prevalence of anal cytological abnormalities. These facts are epidemiologically important for future programs for population eradication of cervical lesions related to HPV. Diagn. Cytopathol. 2011;39:323-327. © 2010 Wiley-Liss, Inc.

show abstract

Section: Discussionmentioning

confidence: 96%

Prevalence of anal cytological abnormalities in women with positive cervical cytology

Calore

Giaccio

Nadal

2011

Diagnostic Cytopathology

View full text Add to dashboard Cite

show abstract

“…Cytobrushes improved cervical sampling over cotton swabs by 58-76% for detecting endocervical cells in a sample of >800 adult females >29 years of age (14) and are shown superior to Ayers spatulas (10). One report suggests the cytobrush may be effective in anal cytology,(15) but may be uncomfortable in blind sampling (16). The NF-swab is evaluated extensively for respiratory-pathogen and -cytology sampling, yielding more cells and pathogens than other collection protocols (17-20).…”

Section: Introductionmentioning

confidence: 99%

Comparison of 2 Anal Cytology Protocols to Predict High-Grade Anal Intraepithelial Neoplasia

Wiley

Hsu

Bolan

et al. 2013

Journal of Lower Genital Tract Disease

View full text Add to dashboard Cite

“…However, for populations in which the prevalence of AIN is lower, little is known about the performance of cytologic screening and its correlation with histology and HPV DNA detection. Published results have varied concerning the sensitivity and specificity of anal cytology,5 and, when available, histologic samples have issued from blinded or forceps biopsy with or without HRA guidance 6, 7. Because AIN often is multifocal, small biopsy specimens, particularly forceps biopsies, may not be sufficient for diagnosis and staging.…”

Section: Introductionmentioning

confidence: 99%

Value of cytologic Papanicolaou smears and polymerase chain reaction screening for human papillomavirus DNA in detecting anal intraepithelial neoplasia

Etienney

Vuong

Si-Mohamed

et al. 2012

Cancer

View full text Add to dashboard Cite

; and the Cytological Diaconesses Group BACKGROUND: The performance of cytologic screening and its correlation with histology and polymerase chain reaction (PCR) detection of human papillomavirus (HPV) DNA have not been evaluated in populations with a low prevalence of anal intraepithelial neoplasia (AIN). The objective of the current study was to analyze the significance of abnormal smears relative to the histology and PCR detection of HPV DNA. METHODS: A cytologic smear and a viral sample were taken in 300 consecutive patients undergoing surgery (Milligan-Morgan hemorrhoidectomy and/or fissurectomy) who gave their informed consent. RESULTS: The cytologic smear was normal in 216 of 290 patients (74.5%). Four high-grade and 19 low-grade intraepithelial neoplastic lesions were identified. In 5 patients, high-grade lesions could not be excluded, 30 lesions were of undetermined significance, and there were 16 cellular modifications with a non-neoplastic appearance. The PCR test for HPV was positive in 18.7% of patients, and a high-risk genotype was identified in 63.6% of positive samples. Histologic examination of the surgical samples was normal in 92.3% of patients. The 23 AIN samples were distributed as follows: 13 grade 1 AIN (AIN1), 6 AIN2, and 4 AIN3. The sensitivity of cytologic smears and PCR for detecting AIN was 56% and 60.8%, respectively, and specificity was 77% and 84.5%, respectively. Combining the 2 tests increased sensitivity to 78% but decreased specificity to 68%. CONCLUSIONS: Compared with a large surgical sample, anal cytologic Papanicolaou smears and HPV PCR exhibited sensitivity and specificity that varied, depending on the risk of HPV infection and AIN. Positive HPV DNA screening increased with AIN grade, and high-risk HPV testing was particularly helpful. INTRODUCTIONThe cervical Papanicolaou (Pap) smear is known as a good cancer screening test. Its widespread use has enabled a significant reduction in cervical cancer morbidity and mortality. 1 Anal and cervical cancers share similarities, including intraepithelial neoplastic lesions known to progress to cancer and a causative association with human papillomavirus (HPV). 2 The usefulness of anal cytology for detecting anal intraepithelial neoplasia (AIN) has been demonstrated in populations at high risk of cancer of the anal canal, ie, men who have sex with men (MSM) and individuals infected with the human immunodeficiency virus (HIV). 3 Anal Pap smears and high-resolution anoscopy (HRA), thus, have been recommended as screening tests for high-risk populations. 4 However, for populations in which the prevalence of AIN is lower, little is known about the performance of cytologic screening and its correlation with histology and HPV DNA detection. Published results have varied concerning the sensitivity and specificity of anal cytology, 5 and, when available, histologic samples have issued from blinded or forceps biopsy with or without HRA guidance. 6,7 Because AIN often is multifocal, small biopsy specimens, particularly forceps biopsies, may ...

show abstract

Anal cytology in patients with AIDS

Cited by 8 publications

References 15 publications

Prevalence of anal cytological abnormalities in women with positive cervical cytology

Prevalence of anal cytological abnormalities in women with positive cervical cytology

Comparison of 2 Anal Cytology Protocols to Predict High-Grade Anal Intraepithelial Neoplasia

Value of cytologic Papanicolaou smears and polymerase chain reaction screening for human papillomavirus DNA in detecting anal intraepithelial neoplasia

Contact Info

Product

Resources

About