Anal Cloacogenic and Squamous Carcinomas Comparative Histologic Analysis Using In Situ Hybridization for Human Papillomavirus DNA

Wolber, Robert; Dupuis, Beverley; Thiyagaratnam, Pat; Owen, David

doi:10.1097/00000478-199002000-00009

Cited by 24 publications

(7 citation statements)

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“…The sections of colon adjacent to the tumour were free of squamous metaplasia; however, this large neoplasm may have obliterated any evidence of squamous metaplasia. In the anal canal, HPV DNA has been found in some cloacogenic carcinomas suggesting that HPV infection may play a role in tumourigenesis, 10–12 but we were unable to detect HPV DNA in this tumour. We therefore suggest that this tumour likely arose from a totipotential basal cell in the colonic mucosa.…”

Section: Discussioncontrasting

confidence: 54%

Basaloid carcinoma of the colon arising at the splenic flexure

Newell

Penswick²,

Driman

2001

Histopathology

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Section: Discussioncontrasting

confidence: 54%

Basaloid carcinoma of the colon arising at the splenic flexure

Newell

Penswick²,

Driman

2001

Histopathology

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“…" In silu hybridization analysis of anal tumors according to histopathologic type showed seven of 12 SCC to be positive for type 161 1 8 but none of 14 basaloid tumors. 35 In another study, HPV DNA was found in 24 of 126 tumors (1 3 of 23 carcinoma in situ lesions, nine of 47 invasive SCC, and only one of 42 basaloid tumors).36 Thus SCC and basaloid tumors may arise by different mechan i s m~.~~ We detected HPV RNA transcription in 73.2% of 41 epidermoid anal cancers. There was no difference between the rates of HPV RNA detection between tumors classified as basaloid or as SCC, but HPV RNA positivity was significantly lower in the older age group.…”

Section: Discussionmentioning

confidence: 60%

Differing characteristics of human papillomavirus rna-positive and rna-negative anal carcinomas

Higgins

Uzelin

Burrell

et al. 1991

Cancer

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ONGLANDULAR ANAL CARCINOMAS may present as N one of two common histologic patterns, either squamous cell carcinoma (SCC) or basaloid (cloacogenic) carcinoma. The latter is postulated to be derived from the anorectal transition zone, a region analogous to the cervical transformation zone. Both types of tumor are more commonly found in women than men,'.2 and both have similar biologic behaviors. Epidemiologic studies indicate that risk factors for anal carcinoma for women include the lifetime number of sexual partners, smoking, and the presence of antibody to herpes simplex type 2 virus. Anal-receptive intercourse was identified as a risk factor in women in one study' but not in another.2 In men, an increased incidence of anal carcinoma was as- In anal carcinomas, the prevalence of HPV infections in the lesions is less well defined. Papillomavirus antigen was detected in anal carcinomas by one author'' but not by two others.2.'2 In addition HPV DNA was detected in none of 13 cases by in situ hybridi~ation,'~ 26 of 46 (56.5%) cases by southern blot anaIysis,l4 and all four cases (100%) by polymerase chain reaction (PCR). Because there have been no reports of the detection of HPV RNA in anal tumors, we used a sensitive radioisotopically labeled RNA-RNA in situ hybridization procedure to determine the prevalence of HPV RNA in anal carcinomas. 56 1

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“…In this study, using non-isotopic DNA in situ hybridization with optimized section digestion and hybridization conditions, we detected HPV 16 infection in 72 per cent of the carcinomas studied. Previous studies using in situ techniques have shown variable results in the correlation of HPV expression with squamous or basaloid tumour r n o r p h~l o g y .~~~ 1, 16,17 There is one report of failure to demonstrate HPV in basaloid variants of anal carcinoma, using DNA probes,I6 while in squamous carcinomas signal has been shown to be most prominent in superficial areas, corresponding to histological evidence of HPV infection, and rarely within deep and iiivasive elements." However, there are other reports of irregular but widespread expression, particularly in areas of squamous differentiati~n.~..'~ Morphological review of anal carcinomas suggests that the vast majority (98 per cent) show evidence of keratinizing squamous differentiation, with coexpression of basaloid features in two out of three cases and evidence of mucin production in 20 per cent.…”

Section: Discussionmentioning

confidence: 99%

Properties of HPV-Positive and HPV-Negative Anal Carcinomas

Love³

et al. 1996

J. Pathol.

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Evidence of human papillomavirus (HPV) can be found in up to 85 per cent of anal carcinomas. In the vulva, a discrete subset of HPV‐positive carcinomas which show koilocytic morphology and distinct clinical features has recently been identified (warty carcinoma). The morphological and prognostic features of HPV‐positive and HPV‐negative anal carcinomas were compared in this study of the tumour distribution of HPV DNA. Vulval and anal neoplasia are similar in many ways and we have also looked to see if their similarity extends to ‘warty’ morphology in relation to HPV status. Thirty‐five resection specimens of anal carcinoma were examined with biotin‐labelled probes for HPV 6, 11, 16, and 18 DNA, using a non‐isotopic in situ hybridization (ISH) technique. No tumour was found to contain HPV 6, 11, or 18. Twenty‐four (72 per cent) showed positivity for HPV 16 DNA. Staining was homogenous and independent of local squamous, basaloid, or ductal differentiation. The majority of tumours showed staining suggestive of episomal, non‐productive HPV infection. HPV‐positive tumours were more likely to occur in the anal canal than perianally and to show a mixed squamous and basaloid appearance. No difference between the two groups was found in patient age, presence of adjacent dysplasia, ductal differentiation, or prognosis. There was no correlation between condylomatous tumour morphology and HPV 16 DNA positivity; thus, a subset equivalent to vulval warty carcinoma could not be identified.

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Anal Cloacogenic and Squamous Carcinomas Comparative Histologic Analysis Using In Situ Hybridization for Human Papillomavirus DNA

Cited by 24 publications

References 0 publications

Basaloid carcinoma of the colon arising at the splenic flexure

Basaloid carcinoma of the colon arising at the splenic flexure

Differing characteristics of human papillomavirus rna-positive and rna-negative anal carcinomas

Properties of HPV-Positive and HPV-Negative Anal Carcinomas

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