Anaerobic production of volatile fatty acids in the sea musselMytilus edulis L.

Kluytmans, J.H.; Veenhof, P. R.; Zwaan, A. De

doi:10.1007/bf02482838

Cited by 102 publications

(20 citation statements)

References 19 publications

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“…Acetate is an end-product of anaerobic metabolism that can be used as a biomarker of exposure to anoxic conditions with the elevated levels in the biological tissues (Kluytmans et al, 1975;Zwaan and Marrewijk et al, 1973). In this work, the level of Table 1 Significantly up-or down-regulated metabolites involved in various physiological functions (P < 0.05 a ) in gill tissues of Cu-exposed clams after exposures for 24, 48 and 96 h. acetate was significantly reduced in clam gill tissues after exposed to Cu for 24 h, which might imply the reduced anaerobic metabolism in gills.…”

Section: Discussionmentioning

confidence: 99%

Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

Zhang

Liu

You

et al. 2011

Marine Environmental Research

100

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Section: Discussionmentioning

confidence: 99%

Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

Zhang

Liu

You

et al. 2011

Marine Environmental Research

100

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“…Succinate was determined using a modified method based on the determination according to Kmetec (1966). Volatile fatty acids were assayed gaschromatographically according to Kluytmans et al (1975). Free amino acids were analyzed as described by Zurburg and Kluytmans (1980).…”

Section: Determination Of Metabolitesmentioning

confidence: 99%

Aspects of anaerobic metabolism in Anodonta cygnea L.

Holwerda

Veenhof

1984

Comparative Biochemistry and Physiology Part B: Comparative Bio

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Abstract--1. After 6 days of anoxia A. cygnea had produced 1.0 pmole succinate, 2.7 pmole propionate and 1.7/~mole acetate/g of total soft tissue (wet). In addition, 0.35 #mole glutamate had disappeared. No other changes were detectable.2. Concentrations of anaerobic end products and of amino acids differed from organ to organ. Large portions of all end products produced were present in the fluids within the animal.3. Acetate and propionate were also excreted into the surrounding water. 4. In all tissues examined the activity ratio of pyruvate kinase and phosphoenolpyruvate carboxykinase was high 5. The [PEP]0. 5 of pyruvate kinase was relatively low (0.04-0.05 mM at pH 7.5), and the enzyme is not strongly inhibited at decreased pH or in the presence of alanine.6. Anaerobiosis influences this enzyme by decreasing the activity at low substrate concentration.

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“…In the organs succinate was determined spectrophotometrically, as described by Kluytmans et al (1975). Succinate in the hem©lymph was concentrated before assay by extracting 1.0ml of acidified hem©lymph with 3 x 5 ml ethylacetate.…”

Section: Determination Of Succinatementioning

confidence: 99%

“…was dissolved in 1 ml 25% HsPO, before gaschromatographic analysis of the volatile fatty acids according to the method of Kluytmans et al (1975). The excretion of volatile fatty acids in the incubation water was determined by taking aliquots of 250m1, that were lyophilized after addition of l.Oml of 1 M HaOH.…”

Section: Analysis Of Volatile Fatty Acidsmentioning

confidence: 99%

“…The number of end products formed by carbohydrate degradation is still growing. Besides the classical end products like lactate, octopine, succinate and alanine, recently volatile fatty acids and N-carboxyethyl-alanine have been detected in bivalved molluscs (Kluytmans et al, 1975;Collicut & Hochachka, 1977). The nature of the fermentation products can vary per species, but additionally quantitative fluctuations are observed due to factors like time of anoxia, temperature and seasonal cycle.…”

Section: Introductionmentioning

confidence: 99%

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Organ specific changes in energy metabolism due to anaerobiosis in the sea mussel Mytilus edulis (L.)

Zurburg

Kluytmans

1980

Comparative Biochemistry and Physiology Part B: Comparative Bio

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1. Anaerobic energy metabolism was investigated in different organs of Mytilus edulis and the whole animal.2. Succinate accumulates to high levels in most organs but remains low in the hemolymph. 3. After 16 hours propionate accumulation is observed in all organs. Experimental evidence is not sufficient yet to point out organs that produce more propionate than others.4. Acetate is a minor end product. 5. Acetate and propionate are found in the hemolymph in amounts equal to those in the organs. 6. Animals incubated in oxygen-free seawater accumulate more end products than animals exposed to air, in the form of volatile fatty acids that are excreted into the incubation water.7. Alanine and glutamate increase in the posterior adductor muscle. Aspartate decreases in the total animal, posterior adductor muscle and gills, while in the hemolymph decreases in alanine, asparagine, serine, threonine and proline are observed.

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Anaerobic production of volatile fatty acids in the sea musselMytilus edulis L.

Cited by 102 publications

References 19 publications

Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

Aspects of anaerobic metabolism in Anodonta cygnea L.

Organ specific changes in energy metabolism due to anaerobiosis in the sea mussel Mytilus edulis (L.)

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