“…Owing to efficient resource recovery and lessened environmental impact, anaerobic digestion compares favorably with alternative treatments, such as incineration and landfill. 2 Previous work in our laboratory demonstrated that the solubility of lignocellulose substrates could be increased by a combined pretreatment method, thus improving the efficiency of the acidification fermentation process. 3 However, the anaerobic conversion of lignocellulose to methane is a complex process involving many species of bacteria, which predominantly include acidogens and methanogens (including acetogens).…”