Anaerobic Digestion of Industrial Tempeh Wastewater with Sludge from Cow Manure Biogas Digester as Inoculum: Effect of F/M Ratio on the Methane Production

Fransiscus, Yunus; Simangunsong, Tuani Lidiawati

doi:10.18517/ijaseit.11.3.11846

Cited by 3 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The anaerobic digestion process used in this study was a complete mix batch digester maintained at 15 °C. The anaerobic digestion feedstock solution was prepared according to a fixed food to microorganism ratio (F/M), i.e., the VS/VS ratio of pig manure to inoculum was set at 0.75 as, according to Fransiscus and Simangunsong (2021), the appropriate F/M for anaerobic digestion systems should be ≤ 1. The total volume of the feedstock solution was 10 L, comprising 200 g of inocula (in VS), 150 g of pig manure (in VS), and water to make up the rest of the volume.…”

Section: Experimental Methodsmentioning

confidence: 99%

Determining the Microbial Source of Methane Production in Anaerobic Digestion Systems Through High-Throughput Sequencing Technology

et al. 2022

View full text Add to dashboard Cite

Anaerobic digestion is widely used to simultaneously generate biogas while treating different organic wastes. It is difficult to determine the source of CH4 from the complex microbial community structure using traditional microbiological pure culture techniques. Therefore, this study aimed to elucidate the microbial source of CH4 in low-temperature anaerobic digestion systems using the recently developed high-throughput sequencing technology. Herein, anaerobic digestion microbes were domesticated at 15°C and then inoculated into pig manure-containing raw materials in a batch anaerobic digester to form a low-temperature anaerobic digestion system with fermentation controlled at 15°C. Several analytical approaches including abiotic factor analysis and biotic factor analysis (high-throughput sequencing) were applied to investigate the abiotic factors, bacterial communities, and archaeal communities in the low-temperature anaerobic digestion system. The results showed that: 1) The anaerobic digestion lasted for 120 days, with 68.65 L total gas production and 31.19 L CH4 production. 2) The relative abundances of the primary and secondary dominant bacterial operational taxonomic units ranged from 8.02 to 22.84% and 5.62–17.09%, respectively, with 99% similarities to Clostridium cellulovorans (a typical cellulose- and hemicellulose-degrading bacterium) and Terrisporobacter petrolearius (a representative fermentation bacterium), respectively. Moreover, the relative abundance of the primary dominant methanogenic archaeal operational taxonomic unit ranged from 1.03 to 16.85%, with 98% similarity to Methanobacterium beijingense, which is a typical hydrogenotropic methanogen. Based on the low-temperature CH4-producing metabolic pathways of bacterial and methanogenic operational taxonomic units, Methanobacterium beijingense was found to be the primary functional microbe for CH4 production in the 15°C anaerobic digestion system.

show abstract

Section: Experimental Methodsmentioning

confidence: 99%

Determining the Microbial Source of Methane Production in Anaerobic Digestion Systems Through High-Throughput Sequencing Technology

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The biogas fermentation process used in this study was a fully mixed batch process with an anaerobic digestion temperature of 9 °C. In this study, the biogas fermentation feed solution was prepared according to the ratio of raw materials to microorganisms (F/M) [18], that was, the ratio of volatile solids of pig manure to inoculum (VS/VS) of 0.75 [19]. The total volume of the feed liquid was 10 l, in which the amount of inoculum was 200 g (calculated by VS), the amount of pig manure was 150 g (calculated by VS), and water was added to 10 l.…”

Section: Experimental Methodsmentioning

confidence: 99%

Effects of environmental factors on low temperature anaerobic digestion of pig manure

Yang

Wang

Zhao

et al. 2022

Environ. Res. Commun.

View full text Add to dashboard Cite

In this study, we investigated the inhibitory effect of low temperature on biogas fermentation system. Biogas fermentation inoculum was domesticated at 4 C, and subsequently inoculated into pig manure feedstock in a batch biogas fermentation process. A low-temperature biogas fermentation system was maintained at 9 C, and its abiotic factors, bacterial community, and archaeal community were determined. The results showed that (1) the biogas fermentation lasted for 160 days, with a total gas production of 19,150 mL, including N2production of 11,370 mL, CO2production of 3,534 mL, and CH4production of 3,031 mL. (2) The average relative abundance of the primary dominant bacterium operational taxonomic unit (OTU) was 30%, with 100% similarity to Pseudomonas caeni, atypical denitrifying bacterium. The average relative abundance of the secondary dominant bacterium OTU was 20.36%, with 99% similarity to Clostridium cellulovorans, a typical cellulose- and hemicellulose-degrading bacterium. The average relative abundance of the primary dominant methanogenicarchaeon OTU was 7.22%, with 99% similarity to Methanosphaera cuniculi, a typical methylotrophic methanogen, and the secondary dominant methanogenic archaeon OTU had an average relative abundance of 4.15%, with 98% similarity to Methanocorpusculum sinense, a typical hydrogenotrophic methanogen. (3) The primary dominant bacterial genus Pseudomonas exhibited highly significant negative correlations with the dominant biogas-producing microorganisms such as Clostridium, Terrisporobacter, Turicibacter, Methanosphaera, and Methanocorpusculum. We concluded that (1) high concentrations of abiotic factors such as ammonia nitrogen, acetic acid, and propionic acid were important indicators of relatively poor operation of low-temperature biogas fermentation systems, while (2) high numbers of MCGarchaea and Pseudomonas were important biotic factors showing comparatively poor operation of the system.

show abstract

“…Soybean wastewater treatment by anaerobic digester produce methane about 55% and COD removal about 80% (Yu, 2015). Anaerobic digestion processes from tempeh wastewater treatment produced methane 8720 mL and COD removal 67.7% (Fransiscus and Simangunsong, 2021). The other soybean wastewater treatment to reduce COD use biological process using Aspergillus niger pelletization technology found 98.11% COD removal (Zhang, Zhang and Diao, 2017).…”

Section: Wastewater Quality Of Tss Bod Cod and Phmentioning

confidence: 99%

Wastewater Quality and Pollution Load of Each Stage in Tempeh Production

Pramaningsih

Hansen

Praveena

et al. 2022

indones. j. urban. environ. technol.

View full text Add to dashboard Cite

Aim: This study is purpose to understand the chemical and physical characteristics and pollution load in each step of tempeh wastewater: washing, boiling, soaking and mixing. Tempeh is traditional food from soybean fermentation. Tempeh has a lot of healthy contents as protein and vitamin. However, wastewater from tempeh production is discharge to river directly which considered as environmentally damaging. However, the characteristics of wastewater from tempeh production and its level of environmental impacts to environment are limitedly studied. Methodology and Results: Tempeh wastewater was analyzed in chemical and physical parameters. Chemical characteristics analyzed are pH, Biological Oxygen Demand (BOD) and Chemical Oxygen Demand (COD). pH was measured using pH meter, BOD using SNI 6989.72:2009 method and COD using SNI 6989.2:2019 method. The physical parameter analyzed is Total Suspended Solid (TSS) and analyzed using SNI 6989.3: 2019 method. Pollution load calculated by deviation maximum pollution load and pollution load existing. Result of this study findings indicated that the highest BOD 299.40 mg/L and COD 540.66 mg/L was found in soaking step compared to other steps in tempeh production. Highest TSS 655.20 mg/L was found in boiling step. The pH values in all steps were acidic 4 and 5. Allocation Pollution load of: TSS overall exceeded the capacity, BOD and COD overall exceeded the capacity except in washing step (BOD 304.39 kg/day and COD 857.78 kg/day). Conclusion, significance and impact of study: The Highest TSS of tempeh wastewater processes was in boiling step while the highest organic matter was detected in soaking. Further for whole pH were acidic and exceeded the standard. Allocation of pollution load for TSS, BOD and COD exceed the standard except for BOD and COD in washing waste. Overall tempeh wastewater needs treatment to prevent river pollution.

show abstract

Anaerobic Digestion of Industrial Tempeh Wastewater with Sludge from Cow Manure Biogas Digester as Inoculum: Effect of F/M Ratio on the Methane Production

Cited by 3 publications

References 20 publications

Determining the Microbial Source of Methane Production in Anaerobic Digestion Systems Through High-Throughput Sequencing Technology

Determining the Microbial Source of Methane Production in Anaerobic Digestion Systems Through High-Throughput Sequencing Technology

Effects of environmental factors on low temperature anaerobic digestion of pig manure

Wastewater Quality and Pollution Load of Each Stage in Tempeh Production

Contact Info

Product

Resources

About