Anaerobic degradation of aromatic amino acids by the hyperthermophilic archaeon Ferroglobus placidus

Aklujkar, Muktak; Risso, Carla; Smith, Jessica A.; Beaulieu, Derek; Dubay, Ryan; Giloteaux, Ludovic; DiBurro, Kristin; Holmes, Dawn E.

doi:10.1099/mic.0.083261-0

Cited by 33 publications

(25 citation statements)

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“…Further, the ‘ Ca. Kryptonium thompsoni' genomes encode a putative gene complement for the anaerobic degradation of aromatic amino acids or similar compounds, notably represented by a phenylacetyl-CoA oxidoreductase homologous to the hyperthermophilic archaeon Ferroglobus placidus 31 . This feature appears to be the first example of an extremely thermophilic or hyperthermophilic bacterium with the presumptive capacity to completely mineralize aromatic compounds, and holds biotechnological potential as well as implications for carbon cycling within geothermal springs 32 .…”

Section: Resultsmentioning

confidence: 99%

Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs

et al. 2016

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Analysis of the increasing wealth of metagenomic data collected from diverse environments can lead to the discovery of novel branches on the tree of life. Here we analyse 5.2 Tb of metagenomic data collected globally to discover a novel bacterial phylum (‘Candidatus Kryptonia') found exclusively in high-temperature pH-neutral geothermal springs. This lineage had remained hidden as a taxonomic ‘blind spot' because of mismatches in the primers commonly used for ribosomal gene surveys. Genome reconstruction from metagenomic data combined with single-cell genomics results in several high-quality genomes representing four genera from the new phylum. Metabolic reconstruction indicates a heterotrophic lifestyle with conspicuous nutritional deficiencies, suggesting the need for metabolic complementarity with other microbes. Co-occurrence patterns identifies a number of putative partners, including an uncultured Armatimonadetes lineage. The discovery of Kryptonia within previously studied geothermal springs underscores the importance of globally sampled metagenomic data in detection of microbial novelty, and highlights the extraordinary diversity of microbial life still awaiting discovery.

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Section: Resultsmentioning

confidence: 99%

Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs

et al. 2016

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“…Although some Archaeoglobus species are capable of chemolithotrophic iron re-duction, Ferroglobus and Geoglobus species are the only members of the family known to couple the complete oxidation of organic compounds with Fe(III) reduction (19)(20)(21). F. placidus also is unique among the Archaeoglobaceae in that it can transfer electrons from the oxidation of aromatic compounds to Fe(III) (22)(23)(24). Although genes involved in aromatics degradation were identified in the G. acetivorans genome, attempts to grow G. acetivorans on such compounds have been unsuccessful (15).…”

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confidence: 99%

Mechanisms Involved in Fe(III) Respiration by the Hyperthermophilic Archaeon Ferroglobus placidus

Smith

Aklujkar

Risso

et al. 2015

Appl Environ Microbiol

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The hyperthermophilic archaeon Ferroglobus placidus can utilize a wide variety of electron donors, including hydrocarbons and aromatic compounds, with Fe(III) serving as an electron acceptor. In Fe(III)-reducing bacteria that have been studied to date, this process is mediated by c-type cytochromes and type IV pili. However, there currently is little information available about how this process is accomplished in archaea. In silico analysis of the F. placidus genome revealed the presence of 30 genes coding for putative c-type cytochrome proteins (more than any other archaeon that has been sequenced to date), five of which contained 10 or more heme-binding motifs. When cell extracts were analyzed by SDS-PAGE followed by heme staining, multiple bands corresponding to c-type cytochromes were detected. Different protein expression patterns were observed in F. placidus cells grown on soluble and insoluble iron forms. In order to explore this result further, transcriptomic studies were performed. Eight genes corresponding to multiheme c-type cytochromes were upregulated when F. placidus was grown with insoluble Fe(III) oxide compared to soluble Fe(III) citrate as an electron acceptor. Numerous archaella (archaeal flagella) also were observed on Fe(III)-grown cells, and genes coding for two type IV pilin-like domain proteins were differentially expressed in Fe(III) oxidegrown cells. This study provides insight into the mechanisms for dissimilatory Fe(III) respiration by hyperthermophilic archaea.

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“…Amino acids are also degraded by gut bacteria, notably tryptophan [72], a precursor of 5-HT [73]. A growing body of evidence indicates an association between the dysregulated kynurenine arm of tryptophan metabolism and many CNS and GI disorders [74].…”

Section: The Role Of Microbiota-derived Metabolitesmentioning

confidence: 99%

Gut Microbiome-Brain Communications Regulate Host Physiology and Behavior

Lee¹,

Serre²

2015

JNHFS

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cell injury [6], metabolic regulation [7], GI tract development [8], innate and adaptive immune responses, and absorption of nutrients [9,10]. Alterations in microbiota composition and dysregulation of the intestinal mucosa homeostasis have been implicated in the development and progression of pathologies. This compositional change in the microbiota and/ or an abnormality in the interactions between the host and the commensal microbiota is referred to as dysbiosis. Gut microbiota dysbiosis has been linked to chronic low-grade intestinal inflammation and acute intestinal autoimmunity diseases such as Irritable Bowel Syndrome (IBS) and Inflammatory Bowel Disease (IBD) [11,12]. Abnormal microbiota composition is associated with a wide range of metabolic and behavioral disorders, such as anxiety/depression [13][14][15] [26]. GI microbiota dysbiosis might also be involved in the development and persistence of systemic disorders [27]. For example, obesity has been characterized by a decrease in overall diversity [28] and an increase in Proteobacteria abundance and in the Firmicutes to Bacteroidetes ratio [28,29] and microbiota composition is believed to influence energy balance and glucose homeostasis [30][31][32].Evidence that changes in microbiota composition are correlated with metabolic and behavioral disorders has drawn attention to a potential causal role for the microbiota in pathologies and has led to the emergence of the 'microbiota-gutbrain axis' concept [33][34][35]. The gut-brain axis is a bidirectional communication system between the GI tract and the brain [36] via hormonal, immunological, and neural signaling. Information from the GI tract and the intestinal microbiota can reach the peripheral and Central Nervous System (CNS), concurrently the brain is able to influence GI functions such as motility and secretion but also immune responses and cytokine production [36,37]. The Microbiota-Gut-Brain AxisThe gut microbiota can modulate gut-brain axis signaling via direct and indirect mechanisms. The microbiota acts via endocrine, metabolic (bacterial components and metabolites), AbstractThe human gut microbiota contains more than 100 trillion bacteria that, under normal physiological conditions, have beneficial symbiotic interactions with the host. However, a growing body of evidence has shown that alternations in the composition and diversity of the gut microbiota, or dysbiosis, can influence the development and progress of metabolic and neurological disorders. Communication between the microbiota and the brain is a bidirectional system involving endocrine, metabolic (bacterial components and metabolites), immune, and neural pathways. Gut microbiota composition influences the signals transmitted from the gut to the brain. Alternatively, the brain utilizes similar mechanisms, in particular endocrine and neural signaling, to modulate the composition of the gut bacteria. In this review, we describe the recent evidence of gut microbiota interaction with the central nervous system to influence physiological a...

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Anaerobic degradation of aromatic amino acids by the hyperthermophilic archaeon Ferroglobus placidus

Cited by 33 publications

References 86 publications

Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs

Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs

Mechanisms Involved in Fe(III) Respiration by the Hyperthermophilic Archaeon Ferroglobus placidus

Gut Microbiome-Brain Communications Regulate Host Physiology and Behavior

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