An ultrastructural analysis of tissue surrounding a microdialysis probe

Clapp-Lilly, Kimberly L.; Roberts, Rosalinda C.; Duffy, Lawrence K.; Irons, Katherine P.; Hu, Yong; Drew, Kelly L.

doi:10.1016/s0165-0270(99)00064-3

Cited by 157 publications

(152 citation statements)

References 25 publications

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“…The insertion SD supports prior conclusions that probe insertion causes a penetration injury. [28][29][30][31][32][33][34][35][36][37][38][39][40][41] Two additional examples of complete recordings of K + and glucose from single animals are provided in the Supplementary Information document. Figure S1 is an example with needle pricks that produced neither a K + spike nor a glucose dip.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies from the rat striatum have established that probe insertion triggers ischemia in the vicinity of the probe and that a glial barrier eventually surrounds and engulfs the probe, thereby hindering long-term sampling. [28][29][30][31][32][33][34][35][36] Adding DEX to the perfusion fluid reduces ischemia and suppresses gliosis. Consistent with these prior reports, the present study confirms that DEX retrodialysis enhances the performance of microdialysis monitoring also in the rat cortex.…”

Section: Discussionmentioning

confidence: 99%

“…[26][27] Nevertheless, inserting the probe into brain tissue causes a penetration injury. [28][29][30][31][32][33][34][35] In response, the host tissue engulfs the probe in a glial barrier that, once formed, impairs further microdialysis sampling. 32,[36][37][38][39][40][41] This is a limitation for clinical microdialysis, as TBI patients often remain under intensive care for 10 days or more.…”

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confidence: 99%

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Enhancing Continuous Online Microdialysis Using Dexamethasone: Measurement of Dynamic Neurometabolic Changes during Spreading Depolarization

Varner

Leong

Jaquins-Gerstl

et al. 2017

ACS Chem. Neurosci.

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Microdialysis is well established in chemical neuroscience as a mainstay technology for real time intracranial chemical monitoring in both animal models and human patients. The capabilities of microdialysis sampling have the potential to be further enhanced through mitigation of the penetration injury unavoidably caused by the insertion of microdialysis probes into brain tissue. Herein, we show that dexamethasone retrodialysis in the rat cortex enhances the microdialysis detection of K+ and glucose transients induced by spreading depolarization. Once inserted, the probes were perfused continuously (1.67 μL/min) either with or without dexamethasone. Without dexamethasone, glucose transients were too small to reliably quantify at 5 days after probe insertion. With dexamethasone, robust K+ and glucose transients were readily quantified at 2 hrs, 5 days, and 10 days after probe insertion. Although the amplitudes of the K+ transients declined day-to-day following probe insertion, amplitudes of the glucose transients were consistent. Immunohistochemistry and fluorescence microscopy confirm that dexamethasone is highly effective at preventing ischemia and gliosis in the vicinity of microdialysis probes implanted for 10 days.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Enhancing Continuous Online Microdialysis Using Dexamethasone: Measurement of Dynamic Neurometabolic Changes during Spreading Depolarization

Varner

Leong

Jaquins-Gerstl

et al. 2017

ACS Chem. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Because of the small size of the probe, minimal tissue damage occurs during insertion into the brain, and tracts are undetectable under a light microscope (25 ). This is in contrast to larger probes (Ͼ200 m in diameter), such as are used in microdialysis, which cause tissue disruption up to 1.4 mm from the probe site (29 ). Fig.…”

Section: Temporal and Spatial Resolutionmentioning

confidence: 99%

Detecting Subsecond Dopamine Release with Fast-Scan Cyclic Voltammetry in Vivo

et al. 2003

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“…However, this may be an underestimate because uptake competes with diffusion of dopamine to the probe, effectively lowering the sampled concentration (15). Also, because microdialysis probes are relatively large, calculations of basal levels must take into account the damaged tissue layer that forms around the probe (16).…”

Section: Dopaminergic Neuronsmentioning

confidence: 99%