An ultrasensitive squamous cell carcinoma antigen biosensing platform utilizing double-antibody single-channel amplification strategy

Ren, Xiang; Wu, Dan; Wang, Yuhuan; Zhang, Yunhui; Fan, Dawei; Pang, Xuehui; Li, Yueyun; Du, Bin; Wei, Qin

doi:10.1016/j.bios.2015.05.012

Cited by 27 publications

(13 citation statements)

References 18 publications

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“…And the detection limit was estimated to be 0.25 pg·mL −1 (S/N = 3). The detection limit was lower than those in some previous reports 2 26 27 28 29 , such as 2.8 pg·mL −1 , 0.02 ng·mL −1 , 1.0 pg·mL −1 , 8.53 pg·mL −1 and 0.17 ng·mL −1 . The results indicated enough sensitivity for monitoring SCCA.…”

Section: Resultscontrasting

confidence: 66%

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A Novel Controlled Release Immunosensor based on Benzimidazole Functionalized SiO2 and Cyclodextrin Functionalized Gold

Wang

et al. 2016

Sci Rep

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A novel controlled release system-based sandwich-type immunosensor is fabricated to detect squamous cell carcinoma antigen (SCCA). The 1-methyl-1H-benzimidazole functionalized mesoporous SiO2 (MBI-MS) is used to load methylene blue (MB). β-cyclodextrin functionalized gold (CD-Au) is introduced as the gatekeeper for encapsulating MB and capturing the adamantly functional detection antibody (ADA-Ab2). And pH stimulus serves as the trigger system to control the MB release. After the load of MB, the CD-Au blocks the pores of the MBI-MS by the host-guest interaction in the neutral condition. However, when the pH is below 7.0, CD-Au is separated from the surface of MBI-MS owing to the protonation of the aromatic amines. The encapsulated MB is released from the pores of MBI-MS and detected by square wave voltammetry. The controlled release immunosensor shows a relatively wide linear range from 0.001 to 20 ng·mL−1 with a low detection limit of 0.25 pg·mL−1. The immunosensor also shows good reproducibility and selectivity, which endows it broad application prospect in clinical research.

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Section: Resultscontrasting

confidence: 66%

“…Squamous cell carcinoma antigen (SCCA), as a member of the serine protease inhibitors family, is often used as a tumour marker with squamous cell carcinoma 1 2 . The SCCA normally exists in basal and parabasal layers of normal squamous epithelium with a low level, but it is found to be overexpressed in epithelia of cancerous tissue 3 .…”

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confidence: 99%

A Novel Controlled Release Immunosensor based on Benzimidazole Functionalized SiO2 and Cyclodextrin Functionalized Gold

Wang

et al. 2016

Sci Rep

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“…Another method is based on the functionalization of the MeNPs with bifunctional linkers (carboxyl-thiols, amine-thiols) or adapter molecules (streptavidin, biotin) and making them react with the Ab via EDC/NHS chemistry (Figure 2c) [16,19]. Several works employing Au, Pt, Pd, Cu, Ag nanoparticles as electrode materials are found in the literature [20][21][22][23][24][25][26][27][28][29]. However, despite their numerous features, MeNPs are not suitable for sufficient signal amplification by themselves [30].…”

Section: Metallic Nanoparticlesmentioning

confidence: 99%

Nanostructure-Based Electrochemical Immunosensors as Diagnostic Tools

et al. 2021

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Electrochemical immunosensors are affinity-based biosensors characterized by several useful features such as specificity, miniaturizability, low cost and simplicity, making them very interesting for many applications in several scientific fields. One of the significant issues in the design of electrochemical immunosensors is to increase the system’s sensitivity. Different strategies have been developed, one of the most common is the use of nanostructured materials as electrode materials, nanocarriers, electroactive or electrocatalytic nanotracers because of their abilities in signal amplification and biocompatibility. In this review, we will consider some of the most used nanostructures employed in the development of electrochemical immunosensors (e.g., metallic nanoparticles, graphene, carbon nanotubes) and many other still uncommon nanomaterials. Furthermore, their diagnostic applications in the last decade will be discussed, referring to two relevant issues of present-day: the detection of tumor markers and viruses.

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“…The effect of these foreign species under optimized CL conditions was determined by analyzing the standard solution of zolpidem (61.4 ng mL -1 ) in presence of these compounds. The tolerance of each substance was taken as the largest concentration of substance yielding an error of less than 3σ in the analytical signal of 61.4 ng mL -1 of zolpidem (σ is the standard deviation in the response obtained from 11 times repeating determination of 61.4 ng mL -1 zolpidem) [32]. The results showed that no interference could be detected when the sample include up to a 100 fold Al 3+ , Fe 2+ , K + , Ca 2+ , Mg 2+ Na + , NO3 -, Starch, 10 folds of sucrose, and uric acid.…”

Section: Interference Studymentioning

confidence: 99%

“…CL methods have found extensive applications in many interesting areas, but their main disadvantages are usually related, in general, with their poor selectivity [25]. Some ultrasensitive methods also have potential for determining the target molecules [28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence System of Permanganate-Sulfite for Simple Determination of Zolpidem

Mokhtari¹,

Aaghamohammadhasan²

2016

Eurasian J Anal Chem

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Zolpidem is an imidazopyridine agent for short term treatment of insomnia with a favorable adverse effect profile. A novel chemiluminescence (CL) method has been proposed for simple determination of zolpidem. The method is based on the fact that zolpidem can produce CL emission in the system of acidic KMnO4 (permanganate) and Na2SO3 (sulfite). On the optimized conditions of chemical variables, CL intensity was proportional to concentration of zolpidem in the range 2.5-295.1 ng mL-1. Limit of detection was 1.6 ng mL-1 (S/N=3). The percent of relative standard deviation was 4.5% for 98.4 ng mL-1 zolpidem. The proposed method was satisfactorily applied for the determination of zolpidem in pharmaceutical formulations and human plasma samples. Sampling rate of the method was calculated about 30 samples h-1. CL mechanism has been proposed using UV-Vis and CL spectra.

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An ultrasensitive squamous cell carcinoma antigen biosensing platform utilizing double-antibody single-channel amplification strategy

Cited by 27 publications

References 18 publications

A Novel Controlled Release Immunosensor based on Benzimidazole Functionalized SiO2 and Cyclodextrin Functionalized Gold

A Novel Controlled Release Immunosensor based on Benzimidazole Functionalized SiO2 and Cyclodextrin Functionalized Gold

Nanostructure-Based Electrochemical Immunosensors as Diagnostic Tools

Chemiluminescence System of Permanganate-Sulfite for Simple Determination of Zolpidem

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