An ultrasensitive fluorescence assay for protein detection by hybridization chain reaction-based DNA nanotags

Signal Amplification for Highly Sensitive Immunosensing

“…18 Schematic illustration of the nanoRCA strategy method showed satisfactory results for linear detection range over 5 orders of magnitude for CEA with a detection limit of 1.2 fg mL -1 . HCR has been coupled with the optical-based immunosensors, including fluorescent [76,77], electrochemiluminescence [78], and bioluminescence [79] for signal amplification by loading large amounts of reporting molecules. For example, Song et al [76] introduced streptavidin QDs (SA-QD) as reporting molecules for the detection of PDGF-BB via HCR based on an aptameric system.…”

Ultrasensitive enzyme-free fluorescent detection of VEGF165 based on target-triggered hybridization chain reaction amplification

“…38 Therefore, HCR has been regarded as an ideal choice in DNA-based signal amplication for protein detection. 39 Graphene oxide (GO) has gained more and more attention due to its large surface area, good water dispersibility and biocompatibility. 40,41 Moreover, GO can strongly adsorb single-stranded nucleic acids, but hardly interacts with rigid double-stranded nucleic acids.…”

Hybridization chain reaction and gold nanoparticles dual signal amplification for sensitive glucose detection

“…In addition, numerous amplification protocols such as nuclease-based target-recycling, rolling circle amplification (RCA), polymerase chain reaction (PCR) and hybridization chain reaction (HCR) have also been utilized for the development of biosensors. Among which, HCR gained considerable attention as a novel amplification strategy and had been applied for the detection of DNA [23][24][25][26][27][28][29][30], protein [31][32][33], metal ions [34,35,17] and cancer cell [36]due to the merits of its including simplicity and high sensitivity,which was driven by the self-assembly of two stable species of DNA hairpins [37] with the high molecular-weight with a lengthcorresponding to over 1000 base pairs (bp), structural flexibilitywith high intrinsic viscosity and rich negative charges.However, to the best of our knowledge, no study has been reported about the glucose detection by using such HCR amplification strategy.…”