An ultrasensitive chemiluminescent ELISA for determination of chloramphenicol in milk, milk powder, honey, eggs and chicken muscle

Tao, Xiaoqi; Jiang, Haiyang; Zhu, Jinghui; Wang, Xia; Wang, Zhanhui; Niu, Lanlan; Wu, Xiaoping; Shi, Weimin; Shen, Jianzhong

doi:10.1080/09540105.2012.753513

Cited by 38 publications

(25 citation statements)

References 16 publications

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“…However, long‐term intake of a trace of chloramphenicol can make anemia, leucopenia, agranulocytosis and aplastic anemia . Hence, the detection of chloramphenicol has been employed in various detection strategies including chromatography tandem mass spectrometry , fluorimetry , colorimetry and chemiluminescence . Chloramphenicol undergoes an irreversible electrochemical reduction at negative potential to get formation of hydroxylamine.…”

Section: Introductionmentioning

confidence: 79%

The Signal Amplification in Electrochemical Detection of Chloramphenicol Using Sulfonated Polyaniline‐chitosan Composite as Redox Capacitor

et al. 2018

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In this work, a novel redox capacitor was designed for signal amplification in electrochemical detection. It was fabricated by co‐electrodeposition of a conducting polymer, sulfonated polyaniline (SPAN) and chitosan on a glass carbon electrode, and its function was evaluated for being a localized source to transfer electron between FcCOOH (Fc) and Ru(NH3)6Cl3 in solution via redox cycling. Furthermore, the electrochemical detection of chloramphenicol, a broad‐spectrum antibiotic was performed using the redox capacitor in the presence of Fc. A significant amplification in cathodic current response of chloramphenicol was obtained through a continuous redox‐cycling reaction. The performance of the amplifying signal responded linearly to chloramphenicol in a concentration range of 0.05 to 50.0 μmol L−1 with a low detection limit of 0.01 μmol L−1. The proposed approach exhibited good reproducibility and stability, and could be used for detection of chloramphenicol in eye drops by standard addition method with the recoveries from 96.5 % to 103.0 %.

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Section: Introductionmentioning

confidence: 79%

The Signal Amplification in Electrochemical Detection of Chloramphenicol Using Sulfonated Polyaniline‐chitosan Composite as Redox Capacitor

et al. 2018

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“…A direct competitive CLEIA for CAP previously developed in our laboratory was used to evaluate the analytical performance of the new CL cocktail. Briefly, high‐binding white plates were coated overnight at 4 °C with 100 μL of the polyclonal anti‐CAP serum (PAb) dissolved in buffer a (1.5 µg/mL).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, there is an urgent need to develop a rapid and sensitive method for the determination of CAP at trace levels in animal‐derived food. In a previous study, we developed a competitive direct chemiluminescent enzyme‐liked immunosorbent assay (CL‐ELISA) for detecting CAP residues in milk, milk powder, honey, eggs and chicken muscle using the SuperSignal CL substrate .…”

Section: Introductionmentioning

confidence: 99%

Development of a highly sensitive chemiluminescence enzyme immunoassay using enhanced luminol as substrate

Tao

Wang

et al. 2013

Luminescence

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In this study, a high sensitivity chemiluminescence enzyme immunoassay (CLEIA) based on novel enhancers was developed. Under optimal conditions, we developed an enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP-C) in the presence of 3-(10'-phenothiazinyl) propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORP) as enhancers. The limit of detection of the newly prepared chemiluminescent cocktail for HRP was 0.33 pg/well, which is lower than that of commercial Super Signal substrate. The results showed that this novel chemiluminescent cocktail can significantly increase the light output of HRP-catalyzed ECR, which can be translated into a corresponding improvement in sensitivity. Similar improvements were observed in CLEIA for the determination of chloramphenicol in milk. In addition, the ECR of N-azoles as secondary enhancer was also presented.

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“…PSL-21-HS was conjugated to HRP using an active ester method with some modification (Shrivastav, 2003;Tao et al, 2014). Two hundred microlitres of each of Dioxan, DMF and water were added to 5 mg of PSL-21-HS, 10 mg NHS and 20 mg EDAC.…”

Section: Preparation Of Psl-21-hs-hrp-enzyme Conjugatementioning

confidence: 99%

Homologous ELISA for detection of prednisolone in human serum

Kumar

Singh

Shrivastav

2017

Food and Agricultural Immunology

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An enzyme-linked immunosorbent assay to measure prednisolone have been developed. An antibody was raised in rabbits using prednisolone-21-hemisuccinate (PSL-21-HS) conjugated to bovine serum albumin. Similarly, PSL-21-HS was conjugated to horseradish peroxidase to prepare enzyme conjugate. The developed assay has been validated for sensitivity and effective displacement at 50% of the assay were 0.078 and 2.64 ng/mL, respectively. This assay showed cross-reaction with only 4 steroids i.e. progesterone 1.76%, 17α-OH progesterone 5.89%, cortisol 7.69% and prednisone 1.13%, out of 55 analogous steroids. The percent recovery of prednisolone from the exogenously spiked human serum pools was in the range of 94.84-100.17%. The intraassay and inter-assay coefficients of variation ranged from 5.79% to 8.00% and from 3.23% to 8.63%, respectively. The serum prednisolone values obtained by this method correlated well with the commercially available kit and found to be 0.93.

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An ultrasensitive chemiluminescent ELISA for determination of chloramphenicol in milk, milk powder, honey, eggs and chicken muscle

Cited by 38 publications

References 16 publications

The Signal Amplification in Electrochemical Detection of Chloramphenicol Using Sulfonated Polyaniline‐chitosan Composite as Redox Capacitor

The Signal Amplification in Electrochemical Detection of Chloramphenicol Using Sulfonated Polyaniline‐chitosan Composite as Redox Capacitor

Development of a highly sensitive chemiluminescence enzyme immunoassay using enhanced luminol as substrate

Homologous ELISA for detection of prednisolone in human serum

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