An ultramicro technique for the determination of deoxypentose nucleic acid

“…The activity of B-glucuronidase contained in the neutrophils was expressed per 1000 pg of the DNA content of each sample. DNA was measured by the indole method (17) Neutrophil chemotaxis. Chemotaxis was assayed using the agarose method (18).…”

Section: Methodsmentioning

confidence: 99%

Neutrophil Function in an Experimental Model of Hemolytic Uremic Syndrome

1987

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“…Mercaptoethanol and iodoacetic acid were likewise added to the protein standards. Deoxyribonucleic acid (DNA) was determined both with diphenylamine (9) and with Keck's modification of the Ceriotti procedure (12). These values, which were in close agreement, were averaged.…”

Section: Methodsmentioning

confidence: 99%

Comparative Physiological Effects of Incorporated Amino Acid Analogs In Escherichia coli

Pine

1978

Antimicrob Agents Chemother

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The relative toxicities of several incorporated analogs of phenylalanine, methionine, arginine, and proline were assessed by a variety of criteria in a derivative of Escherichia coli 15 requiring the antagonized amino acids. Toxicity of the analog-substituted cell protein was most consistently indicated by its insolubility at graded temperatures, its increased breakdown, the relative suppression of further cell growth, and lethality. The relative toxicity of poorly utilized analogs could be judged clearly only by the first two criteria. Toxicity generally increased as follows: selenomethionine < 2,5-dihydrophenylalanine and m-fluorophenylalanine < o-fluorophenylalanine and norleucine < ethionine < p-fluorophenylalanine < azetidine-2-carboxylate < canavanine. The overall perturbation of cell protein structure indicated by the toxicity of the methionine and phenylalanine analogs correlated with their alteration of charge and bulk and was greatly modified by minor positional modifications of fluorine. Among the more specific functional impairments, the activity and heat stability of fl-galactosidase were lowered in parallel by substitutions of phenylalanine and methionine analogs, but not in the usual order of toxicity. Flagella were transiently motile with p-fluorophenylalanine, moderately motile with m-fluorophenylalanine, and fully motile with all methionine analogs. Usually the analog incorporations were no more than bacteriostatic in E. coli strains, canavanine killing only the E. coli 15 substrain extensively in minimal media. Selenomethionine supported indefinite growth of procaryotes such as Bacillus subtilis and certain E. coli strains, but only upon supplementation, at least initially, with many nonessential metabolites.

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An ultramicro technique for the determination of deoxypentose nucleic acid

Cited by 265 publications

References 5 publications

Properties of the Nucleic Acid of the Ryan Strain of Filamentous Influenza Virus

Properties of the Nucleic Acid of the Ryan Strain of Filamentous Influenza Virus

Neutrophil Function in an Experimental Model of Hemolytic Uremic Syndrome

Comparative Physiological Effects of Incorporated Amino Acid Analogs In Escherichia coli

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