An ultra-sensitive colorimetric detection of tetracyclines using the shortest aptamer with highly enhanced affinity

Abstract

A shortened 8-mer ssDNA aptamer was successfully truncated for four different tetracyclines with high affinity. The ultrasensitive colorimetric detection of oxytetracycline using this shortened aptamer was possible, which was about 500-fold enhanced compared to that obtained using the original 76-mer aptamer.

Rapid microwave-assisted synthesis of molecularly imprinted polymers on carbon quantum dots for fluorescent sensing of tetracycline in milk

“…S4, ESI †), the excitation energy could transfer from CDs (donor) 12 to Tc (acceptor), which led to deficient excitation and a highly efficient quenching performance [31,37]. Based on the fluorescence resonant energy transfer system, a rapid and sensitive sensing route for Tc was thus proposed.…”

“…Up to now, various analytical methods for the detection of Tc have been developed, 3 such as high performance liquid chromatography (HPLC) [8,9], LC with tandem mass spectrometry (LC-MS) [3,4], enzyme-linked aptamer assay [10,11] and colorimetric analysis [12,13], etc. Generally, HPLC/LC-MS technique shows high sensitivity and identification ability for target analytes, but the requirement of complicated operation procedures and expert technical skill limits their application in routine analysis.…”

The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products

“…The optimization of aptamer primary sequences is one method that could be used to improve the binding affinity of aptamer towards its target. For instance, a 76-mer ssDNA aptamer has been truncated to produce a shortened aptamer (8-mer ssDNA) that has a much higher affinity for four tetracyclines [38]. Therefore, the sensitivity of the colorimetric is 500-fold enhanced compared to that obtained using the 76-mer aptamer.…”

Upconversion luminescence resonance energy transfer-based aptasensor for the sensitive detection of oxytetracycline

“…Biosensors based on aptamers for OTC detection have been developed, such as colorimetric aptasensors [11,12], electrochemical aptasensors [13], and fluorescence aptasensors [14].…”