2016
DOI: 10.4269/ajtmh.13-0768
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An rK28-Based Immunoenzymatic Assay for the Diagnosis of Canine Visceral Leishmaniasis in Latin America

Abstract: Abstract. Direct observation of Leishmania parasites in tissue aspirates has shown low sensitivity for the detection of canine visceral leishmaniasis (VL). Therefore in the last quarter century immunoenzymatic tests have been developed to improve diagnosis. The purpose of this study was to develop a fast recombinant K28 antigen, naked-eye qualitative enzyme-linked immunosorbent assay (VL Ql-ELISA) and a quantitative version (VL Qt-ELISA), and to display it in a kit format, whose cutoff value (0.156) was select… Show more

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Cited by 14 publications
(7 citation statements)
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References 30 publications
(37 reference statements)
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“…In this study, the indirect rK28 ELISA showed 89.5% sensitivity and 92.0% specificity. Similarly, Lauricella et al reported a sensitivity of 91% and specificity of 100%, which are close to the results obtained in the present study. The TR‐DPP® presented a lower sensitivity (79.0%) and a lower specificity (91.5%) than demonstrated by Ribeiro et al This difference could be related to the gold‐standard method adopted in our study, which showed a high level of sensitivity …”
Section: Discussionsupporting
confidence: 92%
“…In this study, the indirect rK28 ELISA showed 89.5% sensitivity and 92.0% specificity. Similarly, Lauricella et al reported a sensitivity of 91% and specificity of 100%, which are close to the results obtained in the present study. The TR‐DPP® presented a lower sensitivity (79.0%) and a lower specificity (91.5%) than demonstrated by Ribeiro et al This difference could be related to the gold‐standard method adopted in our study, which showed a high level of sensitivity …”
Section: Discussionsupporting
confidence: 92%
“…Soluble antigens from axenic amastigotes were equivalent to promastigotes when used as crude lysates [ 52 ]. Recombinant proteins are considered good candidate antigens for field diagnosis because they are easily adsorbed on several scaffold surfaces and yield reproducible results [ 49 , 54 56 ]. Among them, the most successful antigen moving from academic to clinical settings was rK39.…”
Section: The Utility Of Standard and Newer Rapid Diagnostic Tests In mentioning
confidence: 99%
“…This situation leads to the need for better diagnostic tests, mainly for subclinically infected dogs (6). Most studies on the search for better antigens focus on proteins/peptides identified by bioinformatics analyses (7)(8)(9)(10). However, purifying those antigens can be expensive and involve complex methods.…”
Section: Introductionmentioning
confidence: 99%