An rK28-Based Immunoenzymatic Assay for the Diagnosis of Canine Visceral Leishmaniasis in Latin America

Lauricella, Marta A.; Maidana, Cristina Graciela; Frias, Victoria Fragueiro; Romagosa, Carlo M; Negri, Vanesa; Benedetti, Ruben; Sinagra, Angel; Luna, C; Tartaglino, Lilian; Laucella, Susana A.; Reed, Steven G.; Riarte, Adelina

doi:10.4269/ajtmh.13-0768

Cited by 14 publications

(7 citation statements)

References 30 publications

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“…In this study, the indirect rK28 ELISA showed 89.5% sensitivity and 92.0% specificity. Similarly, Lauricella et al reported a sensitivity of 91% and specificity of 100%, which are close to the results obtained in the present study. The TR‐DPP® presented a lower sensitivity (79.0%) and a lower specificity (91.5%) than demonstrated by Ribeiro et al This difference could be related to the gold‐standard method adopted in our study, which showed a high level of sensitivity …”

Section: Discussionsupporting

confidence: 92%

Plasmonic rK28 ELISA improves the diagnosis of canine Leishmania infection

Maciel

Soares

Costa

et al. 2019

Parasite Immunology

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In this study, we evaluated the performance of a new enzyme‐linked immunosorbent assay (ELISA) variant known as indirect “plasmonic ELISA” (pELISA) for the detection of Leishmania spp. infection. Serum samples from 170 dogs from an area where canine leishmaniosis (CanL) is endemic and from 26 healthy dogs from a nonendemic area were tested by indirect pELISA, and the results were compared to those of an indirect ELISA (both with recombinant antigen rK28) and those of an immunochromatographic test (dual‐path platform, TR‐DPP®) using real‐time PCR on blood samples or conjunctival swabs as the gold standard. The pELISA, indirect rK28 ELISA and the TR‐DPP® immunochromatographic test presented sensitivities of 94.7%, 89.5% and 79.0% and specificities of 100%, 92.7% and 91.5%, respectively. The analysis of the results revealed that the specificity of the indirect pELISA was greater than that of the method recommended by the Ministry of Health in Brazil and may increase the feasibility of diagnosis in resource‐constrained countries because it does not require sophisticated instruments to read. Thus, this method can be used as an additional tool for the detection of Leishmania spp. infection in these areas.

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Section: Discussionsupporting

confidence: 92%

Plasmonic rK28 ELISA improves the diagnosis of canine Leishmania infection

Maciel

Soares

Costa

et al. 2019

Parasite Immunology

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“…Soluble antigens from axenic amastigotes were equivalent to promastigotes when used as crude lysates [ 52 ]. Recombinant proteins are considered good candidate antigens for field diagnosis because they are easily adsorbed on several scaffold surfaces and yield reproducible results [ 49 , 54 – 56 ]. Among them, the most successful antigen moving from academic to clinical settings was rK39.…”

Section: The Utility Of Standard and Newer Rapid Diagnostic Tests In mentioning

confidence: 99%

Canine visceral leishmaniasis: Diagnosis and management of the reservoir living among us

et al. 2018

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This article reviews essential topics of canine visceral leishmaniasis (CVL) due to Leishmania infantum infection. It focuses on the current serological and molecular diagnostic methods used in epidemiological research and veterinary clinics to diagnose CVL and includes new point-of-care (POC) tests under development. The efficacy of different treatment regimens on the clinical improvement and infectiousness of dogs is also addressed. In the last section, the review provides a critical appraisal of the effectiveness of different control measures that have been implemented to curb disease transmission.

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“…This situation leads to the need for better diagnostic tests, mainly for subclinically infected dogs (6). Most studies on the search for better antigens focus on proteins/peptides identified by bioinformatics analyses (7)(8)(9)(10). However, purifying those antigens can be expensive and involve complex methods.…”

Section: Introductionmentioning

confidence: 99%

Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs

et al. 2021

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Lipophosphoglycan (LPG), when used as an ELISA target, confers high specificity and sensitivity to the detection of Leishmania infantum antibodies in dogs. Glycoconjugates are economically viable because the yield is very high after extraction/purification. In addition, they are very stable, which allows their use in point-of-care testing without special storage conditions. During the glycoconjugate extraction, a glycoinositolphospholipid (GIPL)-enriched fraction is obtained in similar quantities as LPG. Since GIPLs can be extracted from the same parasite pellet as LPGs, this work aimed to evaluate the immune recognition of GIPLs by Leishmania infantum-infected dogs and its use for canine leishmaniasis (CanL) immunodiagnosis. Like LPG, GIPLs were recognized by sera from L. infantum-infected dogs, but with less sensitivity (83.8%). However, 80% (16/20) of subclinically infected dogs were detected as positive in the assay. Different from LPG, the GIPL-based assay achieved a lower specificity (73.7%) and cross-reactions occurred with T. cruzi and L. braziliensis-infected dogs. Although GIPLs exhibited a similar performance to LPG for subclinically L. infantum-infected dogs, the occurrence of cross-reactivities with other protozoa and a lower sensitivity hinders its use for an immunodiagnostic test. In places where those diseases do not co-exist such as in the Mediterranean region, its use for subclinically dogs could be an alternative.

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An rK28-Based Immunoenzymatic Assay for the Diagnosis of Canine Visceral Leishmaniasis in Latin America

Cited by 14 publications

References 30 publications

Plasmonic rK28 ELISA improves the diagnosis of canine Leishmania infection

Plasmonic rK28 ELISA improves the diagnosis of canine Leishmania infection

Canine visceral leishmaniasis: Diagnosis and management of the reservoir living among us

Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs

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