“…The DAT is a simple sero-diagnostic tool which does not require sophisticated equipment; however, its use may yield, as for IFAT, to false negative results due to low antibody levels or false positive results caused by the cross-reactions between Leishmania species and other protozoan parasites [43,44,55,57,66,75,79,80]. The presence of anti-leishmanial antibodies in the serum of subclinical or clinical CL cases was also analysed by ELISA [41,43,46,47,54,63,74], a serological assay generally considered as highly sensitive which has a variable specificity that depends on the used leishmanial antigen [79]. Thus, the absence of concordance between DAT, ELISA and PCR tests analysing subclinical or clinical cases was observed for some studies [43,44,57].…”