An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2

Sarfatti, M.; Katan, J.; Fluhr, Robert; Zamir, Daniel

doi:10.1007/bf00262574

Cited by 100 publications

(24 citation statements)

References 12 publications

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“…Interestingly, the intact 12 locus displays behavior closer to partial dominance rather than to dominance. Thus, a threefold increase in disease rate in heterozygote (12i2) versus homozygote (1212) plants was observed (Sarfatti et al, 1989). This observation is consistent with a dose-dependent component in the nature of 12 expression.…”

Section: Structural Properties Of 12c and Their Relationship Tosupporting

confidence: 72%

The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes.

et al. 1997

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Section: Structural Properties Of 12c and Their Relationship Tosupporting

confidence: 72%

The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes.

et al. 1997

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“…The results are presented as percentages of diseased or resistant plants, established by visual classi®cation into two categories: healthy, if no external or internal symptoms were found, and diseased, if stunting, wilting or vascular browning was observed. Individual plants in populations that are homozygous for Fusarium resistance can display disease symptoms at a rate of up to 20% due to the variable penetrance of the expression of Fusarium resistance genes in defense, as was shown by Sarfatti et al (1989Sarfatti et al ( , 1991. Similarly, when homozygous susceptible genotypes are inoculated, up to 20% of the plants can escape infection.…”

Section: Analysis Of Resistancementioning

confidence: 93%

Genome-wide dissection of Fusarium resistance in tomato reveals multiple complex loci

et al. 2001

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Resistance to different pathogenic races of Fusarium oxysporum f. sp. lycopersici (F. o. lycopersici) was explored at two genomic levels in tomato. Six independent Fusarium resistance loci were identified by comparing the responses of a complete set of 53 lines carrying different introgressed regions of the Lycopersicon pennellii genome in a L. esculentum background. The loci confer varying degrees of resistance to different races of the pathogen. Corresponding map positions from different tomato species were aligned and in some cases revealed parallel resistance to F. o. lycopersici with qualitative changes in race specificities. One of the loci identified corresponds to the previously characterized complex resistance locus I2, which is involved in resistance to F. o. lycopersici race 2. A novel member of this locus, I2C-5, which belongs to the NBS-LRR family of resistance genes, was cloned and shown to confer partial resistance in transgenic plants. Thus, at a particular complex locus gene members can confer full or partial resistance to F. o. lycopersici race 2. The results of our whole-genome mapping analysis underline the robust independent origin of resistance to a particular disease and demonstrate the conservation of resistance features at syntenic loci, together with the rapid diversification of genes for innate resistance within loci.

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“…In fact, studies that have screened random genomic clones on pairs of NILs have been successful in identifying markers linked to two disease resistance genes in tomato (6,7).…”

mentioning

confidence: 99%

Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines.

Martin

Williams

Tanksley

1991

Proc. Natl. Acad. Sci. U.S.A.

412

131

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An approach to isolating DNA sequences that are linked to important plant genes is described. The strategy is based upon a recent modification of the polymerase chain reaction in which synthetic primers are used to amplify random sequences from genomic DNA. This technique, used in conjunction with near-isogenic lines (which differ only by the presence or absence of the target gene and a small region of surrounding DNA), leads to the rapid identification of sequences linked to the gene of interest. The feasibility of this method has been demonstrated by analyzing a pair of tomato near-isogenic lines that differ for a region on chromosome 5 that contains a gene (Pto) conferring resistance to Pseudomonas syringae pv. tomato. One hundred forty-four random primers were screened on these lines, and seven amplified products were identified that were present in one but not the other line. Of four products that were further investigated, three were confirmed by segregation analysis to be tightly linked to the Pto gene. Linked sequences identified by this method are useful for detecting the presence of the target gene in plant populations (e.g., in plant breeding) and, if very tightly linked, as starting points for a chromosome walk to isolate the gene. Since near-isogenic lines are a typical product of plant breeding and classical genetic studies, this method is applicable to a wide variety of species.

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An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2

Cited by 100 publications

References 12 publications

The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes.

The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes.

Genome-wide dissection of Fusarium resistance in tomato reveals multiple complex loci

Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines.

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