An overview of Trichoderma reesei co-cultures for the production of lignocellulolytic enzymes

Sperandio, Guilherme Bento

doi:10.1007/s00253-021-11261-7

Cited by 28 publications

(8 citation statements)

References 47 publications

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“…Even efficiency during hydrolysis of bamboo and reed pulp exceeded the model strain TR, showing the mutant MEA-12 has high great potential for industrial application. At present, the commercial cellulase solution is mainly composed of cellulase derived from Trichoderma and Aspergillus [ 40 ]. However, the composition of cellulase from different genera is different.…”

Section: Resultsmentioning

confidence: 99%

Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Peng

Lin

et al. 2021

Biotechnol Biofuels

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Background Cellulase plays a key role in converting cellulosic biomass into fermentable sugar to produce chemicals and fuels, which is generally produced by filamentous fungi. However, most of the filamentous fungi obtained by natural breeding have low secretory capacity in cellulase production, which are far from meeting the requirements of industrial production. Random mutagenesis combined with adaptive laboratory evolution (ALE) strategy is an effective method to increase the production of fungal enzymes. Results This study obtained a mutant of Trichoderma afroharzianum by exposures to N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), Ethyl Methanesulfonate (EMS), Atmospheric and Room Temperature Plasma (ARTP) and ALE with high sugar stress. The T. afroharzianum mutant MEA-12 produced 0.60, 5.47, 0.31 and 2.17 IU/mL FPase, CMCase, pNPCase and pNPGase, respectively. These levels were 4.33, 6.37, 4.92 and 4.15 times higher than those of the parental strain, respectively. Also, it was found that T. afroharzianum had the same carbon catabolite repression (CCR) effect as other Trichoderma in liquid submerged fermentation. In contrast, the mutant MEA-12 can tolerate the inhibition of glucose (up to 20 mM) without affecting enzyme production under inducing conditions. Interestingly, crude enzyme from MEA-12 showed high enzymatic hydrolysis efficiency against three different biomasses (cornstalk, bamboo and reed), when combined with cellulase from T. reesei Rut-C30. In addition, the factors that improved cellulase production by MEA-12 were clarified. Conclusions Overall, compound mutagenesis combined with ALE effectively increased the production of fungal cellulase. A super-producing mutant MEA-12 was obtained, and its cellulase could hydrolyze common biomasses efficiently, in combination with enzymes derived from model strain T. reesei, which provides a new choice for processing of bioresources in the future.

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Section: Resultsmentioning

confidence: 99%

Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Peng

Lin

et al. 2021

Biotechnol Biofuels

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“…Trichoderma reesei is one of the most extensively used fungal species to produce cellulolytic enzymes in industry, due to its extraordinary high secretion capacity for enzymes, especially efficient cellulases [16,24,47,61,62]. Still, it lacks sufficient β-glucosidase activity for efficient cellulose hydrolysis and there are numerous examples on supplementation of cellulases from T. reesei with β-glucosidase preparations, especially from Aspergillus niger or other Aspergillus species [4,37,[63][64][65][66]. However, industrial production of cellobiohydrolases and β-glucosidases in separate organisms is expensive because of the requirement for double equipment.…”

Section: Production Of Enzymes For Plant Biomass Utilizationmentioning

confidence: 99%

“…The production of enzymes can be carried out on-site using cheap complex polymeric substrates e.g., containing agricultural sidestreams consisting of plant cell wall material for growth and enzyme production [38,68]. Co-cultivation with various fungi for enzyme production, especially using solid state fermentation (SSF) with different solid agricultural sidestreams, where the fungi do not necessarily have direct contact but can thrive in micro-niches in the substrate, has been shown in several cases to enable production of efficient enzyme cocktails [37,69], reviewed in [66]. These cocktails are likely optimized for hydrolysis of the same substrate that was used to produce them, as their secretomes may be induced specifically by the composition of the polymers present in the substrates.…”

Section: Production Of Enzymes For Plant Biomass Utilizationmentioning

confidence: 99%

Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Lübeck

2022

Microorganisms

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Filamentous fungi are a large and diverse taxonomically group of microorganisms found in all habitats worldwide. They grow as a network of cells called hyphae. Since filamentous fungi live in very diverse habitats, they produce different enzymes to degrade material for their living, for example hydrolytic enzymes to degrade various kinds of biomasses. Moreover, they produce defense proteins (antimicrobial peptides) and proteins for attaching surfaces (hydrophobins). Many of them are easy to cultivate in different known setups (submerged fermentation and solid-state fermentation) and their secretion of proteins and enzymes are often much larger than what is seen from yeast and bacteria. Therefore, filamentous fungi are in many industries the preferred production hosts of different proteins and enzymes. Edible fungi have traditionally been used as food, such as mushrooms or in fermented foods. New trends are to use edible fungi to produce myco-protein enriched foods. This review gives an overview of the different kinds of proteins, enzymes, and peptides produced by the most well-known fungi used as cell factories for different purposes and applications. Moreover, we describe some of the challenges that are important to consider when filamentous fungi are optimized as efficient cell factories.

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“…Although co-cultivating fungi to produce better enzymatic cocktails is not a brand-new idea (Zoglowek et al, 2016 ), the studies within this domain fare poorly compared to their bacterial counterparts in terms of insight. Most experiments grow two to three strains under the same conditions used for the cultivation of one single strain (Sperandio and Filho, 2021 ). The effects of inoculum volume ratio (Rabello et al, 2014 ) and time (Kolasa et al, 2014 ) can be investigated, but this is not common place.…”

Section: The Metabolic Black-box Of Fungal Co-culturesmentioning

confidence: 99%

Co-cultivation, Co-culture, Mixed Culture, and Microbial Consortium of Fungi: An Understudied Strategy for Biomass Conversion

Lima

Lucas

2022

Front. Microbiol.

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An overview of Trichoderma reesei co-cultures for the production of lignocellulolytic enzymes

Cited by 28 publications

References 47 publications

Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Co-cultivation, Co-culture, Mixed Culture, and Microbial Consortium of Fungi: An Understudied Strategy for Biomass Conversion

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