An Overview of the Loading Efficacy of Cationic Lipids with Milk Proteins: A Potential Application for Lipid Delivery

Abstract

<jats:sec> <jats:title /> <jats:p>In this review, the loading efficacies of helper and Cationic Lipids Cholesterol (CHOL), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), Dioctadecyl Dimethyl- Ammonium Bromide (DDAB) and Dioleoyl Phosphatidylethanolamine (DOPE) with milk β- lactoglobulin, α-casein and β-casein were compared in aqueous solution at physiological conditions. Structural analysis showed that lipids bind milk proteins via hydrophilic, hydrophobic and H-bonding contacts with DOTAP and DDAB forming more stable protein conjugates. Loading efficacy was 30-50% and enhanced with cationic lipids. Lipid conjugation altered protein conformation, causing a partial protein structural destabilization. Milk proteins are capable of transporting lipids in vitro.</jats:p> </jats:sec>

Smart Lipid‐Based Nanoparticles in Lung Cancer Treatment: Current Status and Future Directions

“…Liposomes are spherical lipid bilayer membrane vesicles capable of encapsulating hydrophilic or hydrophobic medicines or other compounds. [105,106] Electrostatic interactions can occur between positively charged cationic liposomes, such as those generated using DOTAP, and negatively charged molecules, such as DNA or RNA. A 1,2dioleyl-3-trimetylammonium propane (DOTAP) liposomal formulation is a type of liposomal formulation consisting of DOTAP and additional lipids.…”