An Overview of the Biochemical and Molecular Aspects of Microbial Oxidation of Inorganic Sulfur Compounds

Mohapatra, Bidyut R.; Gould, W. Douglas; Dinardo, O.; Koren, David W.

doi:10.1002/clen.200700213

Cited by 16 publications

(11 citation statements)

References 72 publications

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“…If so, at a minimum, sulfur oxidizing bacteria would be needed to make the sulfate available, followed by deposition of K 2 SO 4 , either by the same organisms or others. Possible aerobic sulfur oxidizing bacteria include Paracoccus, which was isolated here, but the Bacillus, Streptomyces and even Pseudomonas (Mohapatra et al, 2008) members detected here are also possible. Whether any of these, or any of the other identified organisms deposit K 2 SO 4 remains unknown, however.…”

Section: Discussionmentioning

confidence: 70%

The microbiomes of a XVIII century mummy from the castle of Krásna Hôrka (Slovakia) and its surrounding environment

Kraková

Šoltýs

Puškárová

et al. 2018

Environmental Microbiology

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This microbiological survey was performed to determine the conservation state of a mummy in the Slovak castle of Krásna Hôrka and its surrounding environment. Culture-dependent identification was coupled with biodegradation assays on keratin, gelatin and cellulose. Next Generation Sequencing (NGS) using Illumina platform was used for a deeper microbial investigation. Three environmental samples were collected: from the glass of the sarcophagus, from the air inside it, and from the air of the chapel where the mummy is located. Seven different samples were taken from mummy's surface: from the left ear, left-hand palm, left-hand nail, left instep, right hand, abdomen and mineral crystals embedded within the skin. Three internal organ samples, from the lung, pleura and stomach, were also included in this study. Together, the culture-dependent and culture-independent analyses revealed that the bacterial communities present had fewer taxa than the fungal ones. The mycobiome showed the largest variability and included Epicoccum nigrum, Penicillium spp., Alternaria spp., Aspergillus spp., Cladosporium spp. and Aureobasidium pullulans; many other Ascomycota and Basidiomycota genera were detected by NGS. The most interesting results came from the skin mineral crystals and the internal organs. The hydrolytic assays revealed those microorganisms which might be considered dangerous 'mummy pathogens'. © 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.

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Section: Discussionmentioning

confidence: 70%

The microbiomes of a XVIII century mummy from the castle of Krásna Hôrka (Slovakia) and its surrounding environment

Kraková

Šoltýs

Puškárová

et al. 2018

Environmental Microbiology

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“…The replacement of the SAR as the central reaction to oxidize RISCs to sulfate as usually proposed in the literature dealing with acidophilic bacteria (Mohapatra et al, 2008; Rohwerder and Sand, 2007) with thiosulfate oxidation by the Sox system constitutes an important contribution and a paradigm shift to the understanding of RISCs oxidation in At. thiooxidans .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it is widely recognized that the oxidation of RISCs to sulfuric acid is of great importance for biohydrometallurgical technologies. For example, the inhibition and promotion of biochemical steps in elemental sulfur oxidation pathways is highly relevant for bioleaching operations (Mohapatra et al, 2008; Rohwerder and Sand, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…There is some agreement on two fundamental oxidation processes for sulfide, sulfur, and thiosulfate biooxidation: one mechanism not involving polythionates which is found mainly in neutrophilic microorganisms, and a second one present in neutrophilic as well as acidophilic chemolithotrophic sulfur bacteria and archaea, able to obtain energy to support growth from the oxidation of RISCs involving polythionates as pathway intermediates (reviewed in Friedrich et al, 2005; Ghosh and Dam, 2009; Mohapatra et al, 2008; Rohwerder and Sand, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Stoichiometric modeling of oxidation of reduced inorganic sulfur compounds (Riscs) in Acidithiobacillus thiooxidans

Fazzini

Cortés

Padilla

et al. 2013

Biotech & Bioengineering

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The prokaryotic oxidation of reduced inorganic sulfur compounds (RISCs) is a topic of utmost importance from a biogeochemical and industrial perspective. Despite sulfur oxidizing bacterial activity is largely known, no quantitative approaches to biological RISCs oxidation have been made, gathering all the complex abiotic and enzymatic stoichiometry involved. Even though in the case of neutrophilic bacteria such as Paracoccus and Beggiatoa species the RISCs oxidation systems are well described, there is a lack of knowledge for acidophilic microorganisms. Here, we present the first experimentally validated stoichiometric model able to assess RISCs oxidation quantitatively in Acidithiobacillus thiooxidans (strain DSM 17318), the archetype of the sulfur oxidizing acidophilic chemolithoautotrophs. This model was built based on literature and genomic analysis, considering a widespread mix of formerly proposed RISCs oxidation models combined and evaluated experimentally. Thiosulfate partial oxidation by the Sox system (SoxABXYZ) was placed as central step of sulfur oxidation model, along with abiotic reactions. This model was coupled with a detailed stoichiometry of biomass production, providing accurate bacterial growth predictions. In silico deletion/inactivation highlights the role of sulfur dioxygenase as the main catalyzer and a moderate function of tetrathionate hydrolase in elemental sulfur catabolism, demonstrating that this model constitutes an advanced instrument for the optimization of At. thiooxidans biomass production with potential use in biohydrometallurgical and environmental applications.

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“…It is an autotrophic Gram-negative bacterium that obtains energy from the oxidation of reduced inorganic sulfur compounds (RISC). Acidithiobacillus thiooxidans capacity to produce sulfuric acid, especially during the control of biochemical steps related to elemental sulfur oxidation pathways and the acidification of the media (Mohapatra et al, 2008) have positioned this bacterium as one of the most studied organism in the field of bioleaching processes (Chen et al, 2015;Yan et al, 2015;Quatrini et al, 2017;Zhou et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Integration of Biological Networks for Acidithiobacillus thiooxidans Describes a Modular Gene Regulatory Organization of Bioleaching Pathways

Cortés

Acuña

Travisany

et al. 2020

Front. Mol. Biosci.

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Acidithiobacillus thiooxidans is one of the most studied biomining species, highlighting its ability to oxidize reduced inorganic sulfur compounds, coupled with its elevated capacity to live under an elevated concentration of heavy metals. In this work, using an in silico semi-automatic genome scale approach, two biological networks for A. thiooxidans Licanantay were generated: (i) An affinity transcriptional regulatory network composed of 42 regulatory family genes and 1,501 operons (57% genome coverage) linked through 2,646 putative DNA binding sites (arcs), (ii) A metabolic network reconstruction made of 523 genes and 1,203 reactions (22 pathways related to biomining processes). Through the identification of confident connections between both networks (V-shapes), it was possible to identify a sub-network of transcriptional factor (34 regulators) regulating genes (61 operons) encoding for proteins involved in biomining-related pathways. Network analysis suggested that transcriptional regulation of biomining genes is organized into different modules. The topological parameters showed a high hierarchical organization by levels inside this network (14 layers), highlighting transcription factors CysB, LysR, and IHF as complex modules with high degree and number of controlled pathways. In addition, it was possible to identify transcription factor modules named primary regulators (not controlled by other regulators in the sub-network). Inside this group, CysB was the main module involved in gene regulation of several bioleaching processes. In particular, metabolic processes related to energy metabolism (such as sulfur metabolism) showed a complex integrated regulation, where different primary regulators controlled several genes. In contrast, pathways involved in iron homeostasis and oxidative stress damage are mainly regulated by unique primary regulators, conferring Licanantay an efficient, and specific metal resistance response. This work shows new evidence in terms of transcriptional regulation at a systems level and broadens the study of bioleaching in A. thiooxidans species.

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An Overview of the Biochemical and Molecular Aspects of Microbial Oxidation of Inorganic Sulfur Compounds

Cited by 16 publications

References 72 publications

The microbiomes of a XVIII century mummy from the castle of Krásna Hôrka (Slovakia) and its surrounding environment

The microbiomes of a XVIII century mummy from the castle of Krásna Hôrka (Slovakia) and its surrounding environment

Stoichiometric modeling of oxidation of reduced inorganic sulfur compounds (Riscs) in Acidithiobacillus thiooxidans

Integration of Biological Networks for Acidithiobacillus thiooxidans Describes a Modular Gene Regulatory Organization of Bioleaching Pathways

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